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Adiponectin and leptin induce VCAM-1 expression in human and murine chondrocytes.

Conde J, Scotece M, López V, Gómez R, Lago F, Pino J, Gómez-Reino JJ, Gualillo O - PLoS ONE (2012)

Bottom Line: Osteoarthritis (OA) and rheumatoid arthritis (RA), the most common rheumatic diseases, are characterized by irreversible degeneration of the joint tissues.In addition, both adipokines have additive effect with IL-1β.Finally, we demonstrate that several kinases, including JAK2, PI3K and AMPK are at a play in the intracellular signalling of VCAM-1 induction.

View Article: PubMed Central - PubMed

Affiliation: NEIRID Lab (NeuroEndocrine Interaction in Rheumatology and Inflammatory Diseases), SERGAS, Santiago University Clinical Hospital, Institute of Medical Research (IDIS), Santiago de Compostela, Spain.

ABSTRACT

Background: Osteoarthritis (OA) and rheumatoid arthritis (RA), the most common rheumatic diseases, are characterized by irreversible degeneration of the joint tissues. There are several factors involved in the pathogenesis of these diseases including pro-inflammatory cytokines, adipokines and adhesion molecules.

Objective: Up to now, the relationship between adipokines and adhesion molecules at cartilage level was not explored. Thus, the aim of this article was to study the effect of leptin and adiponectin on the expression of VCAM-1 in human and murine chondrocytes. For completeness, intracellular signal transduction pathway was also explored.

Methods: VCAM-1 expression was assessed by quantitative RT-PCR and western blot analysis upon treatment with leptin, adiponectin and other pertinent reagents in cultured human primary chondrocytes. Signal transduction pathways have been explored by using specific pharmacological inhibitors in the adipokine-stimulated human primary chondrocytes and ATDC5 murine chondrocyte cell line.

Results: Herein, we demonstrate, for the first time, that leptin and adiponectin increase VCAM-1 expression in human and murine chondrocytes. In addition, both adipokines have additive effect with IL-1β. Finally, we demonstrate that several kinases, including JAK2, PI3K and AMPK are at a play in the intracellular signalling of VCAM-1 induction.

Conclusions: Taken together, our results suggest that leptin and adiponectin could perpetuate cartilage-degrading processes by inducing also factors responsible of leukocyte and monocyte infiltration at inflamed joints.

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Related in: MedlinePlus

Determination of mVCAM-1 mRNA and protein expression by quantitative real-time PCR and western blot.A. mVCAM-1 expression after leptin (400 and 800 nM) treatment in ATDC-5 cell line, during 24 hours. B. mVCAM-1 mRNA expression after IL-1β (10 ng/ml), leptin (800 nM) and the combination of IL-1β (10 ng/ml) plus leptin (800 nM) challenge in ATDC-5 cell line, during 24 hours.
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pone-0052533-g004: Determination of mVCAM-1 mRNA and protein expression by quantitative real-time PCR and western blot.A. mVCAM-1 expression after leptin (400 and 800 nM) treatment in ATDC-5 cell line, during 24 hours. B. mVCAM-1 mRNA expression after IL-1β (10 ng/ml), leptin (800 nM) and the combination of IL-1β (10 ng/ml) plus leptin (800 nM) challenge in ATDC-5 cell line, during 24 hours.

Mentions: As shown in figure 4A, leptin was able to induce VCAM-1 expression in a dose- dependent manner.


Adiponectin and leptin induce VCAM-1 expression in human and murine chondrocytes.

Conde J, Scotece M, López V, Gómez R, Lago F, Pino J, Gómez-Reino JJ, Gualillo O - PLoS ONE (2012)

Determination of mVCAM-1 mRNA and protein expression by quantitative real-time PCR and western blot.A. mVCAM-1 expression after leptin (400 and 800 nM) treatment in ATDC-5 cell line, during 24 hours. B. mVCAM-1 mRNA expression after IL-1β (10 ng/ml), leptin (800 nM) and the combination of IL-1β (10 ng/ml) plus leptin (800 nM) challenge in ATDC-5 cell line, during 24 hours.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3526577&req=5

pone-0052533-g004: Determination of mVCAM-1 mRNA and protein expression by quantitative real-time PCR and western blot.A. mVCAM-1 expression after leptin (400 and 800 nM) treatment in ATDC-5 cell line, during 24 hours. B. mVCAM-1 mRNA expression after IL-1β (10 ng/ml), leptin (800 nM) and the combination of IL-1β (10 ng/ml) plus leptin (800 nM) challenge in ATDC-5 cell line, during 24 hours.
Mentions: As shown in figure 4A, leptin was able to induce VCAM-1 expression in a dose- dependent manner.

Bottom Line: Osteoarthritis (OA) and rheumatoid arthritis (RA), the most common rheumatic diseases, are characterized by irreversible degeneration of the joint tissues.In addition, both adipokines have additive effect with IL-1β.Finally, we demonstrate that several kinases, including JAK2, PI3K and AMPK are at a play in the intracellular signalling of VCAM-1 induction.

View Article: PubMed Central - PubMed

Affiliation: NEIRID Lab (NeuroEndocrine Interaction in Rheumatology and Inflammatory Diseases), SERGAS, Santiago University Clinical Hospital, Institute of Medical Research (IDIS), Santiago de Compostela, Spain.

ABSTRACT

Background: Osteoarthritis (OA) and rheumatoid arthritis (RA), the most common rheumatic diseases, are characterized by irreversible degeneration of the joint tissues. There are several factors involved in the pathogenesis of these diseases including pro-inflammatory cytokines, adipokines and adhesion molecules.

Objective: Up to now, the relationship between adipokines and adhesion molecules at cartilage level was not explored. Thus, the aim of this article was to study the effect of leptin and adiponectin on the expression of VCAM-1 in human and murine chondrocytes. For completeness, intracellular signal transduction pathway was also explored.

Methods: VCAM-1 expression was assessed by quantitative RT-PCR and western blot analysis upon treatment with leptin, adiponectin and other pertinent reagents in cultured human primary chondrocytes. Signal transduction pathways have been explored by using specific pharmacological inhibitors in the adipokine-stimulated human primary chondrocytes and ATDC5 murine chondrocyte cell line.

Results: Herein, we demonstrate, for the first time, that leptin and adiponectin increase VCAM-1 expression in human and murine chondrocytes. In addition, both adipokines have additive effect with IL-1β. Finally, we demonstrate that several kinases, including JAK2, PI3K and AMPK are at a play in the intracellular signalling of VCAM-1 induction.

Conclusions: Taken together, our results suggest that leptin and adiponectin could perpetuate cartilage-degrading processes by inducing also factors responsible of leukocyte and monocyte infiltration at inflamed joints.

Show MeSH
Related in: MedlinePlus