Limits...
Evaluation of anxiolytic activity of compound Valeriana jatamansi Jones in mice.

You JS, Peng M, Shi JL, Zheng HZ, Liu Y, Zhao BS, Guo JY - BMC Complement Altern Med (2012)

Bottom Line: The effects of the compound on spontaneous activity were evaluated by locomotor activity test.We further investigated the mechanism of action underlying the anxiolytic-like effect of compound by pre-treating animals with antagonists of benzodiazepine (flumazenil, 3mg/kg) prior to evaluation using EPM and LDB.However, the anxiolytic-like effects of compound were significantly reduced by pre-treatment with flumazenil (P>0.05).

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, PR China.

ABSTRACT

Background: Compound Valeriana jatamansi Jones is a formula for treating anxiety-related diseases in the clinic, which is composed of Valeriana jatamansi Rhizoma et Radix, Ziziphi Spinosae Semen, Albiziae Cortex and Junci Medulla. The purpose of this study was to explore the anxiolytic properties of this compound in mice.

Methods: Male ICR mice were treated with compound Valerianae Jatamansi Jones (1.2 g/kg, 2.4 g/kg, 4.8 g/kg), saline, diazepam (2 mg/kg) orally for 10 days and then exposed to elevated maze-plus (EPM) and light-dark box (LDB). The effects of the compound on spontaneous activity were evaluated by locomotor activity test. We further investigated the mechanism of action underlying the anxiolytic-like effect of compound by pre-treating animals with antagonists of benzodiazepine (flumazenil, 3mg/kg) prior to evaluation using EPM and LDB.

Results: Compound Valerianae Jatamansi Jones (2.4, 4.8 g/kg, p.o.) significantly increased entries (P<0.05) into and time spent (P<0.05) on the open arms of the EPM, and number of transitions (P<0.05) and time spent (P<0.05) in the light compartment of the LDB. However, the anxiolytic-like effects of compound were significantly reduced by pre-treatment with flumazenil (P>0.05). In addition, compound Valerianae Jatamansi Jones treatment didn't affect the spontaneous activity in mice (P> 0.05).

Conclusions: The present study supports the hypothesis that compound Valeriana jatamansi Jones exert anxiolytic action but no sedative effects in mice and that this effect might be mediated by benzodiazepine receptors.

Show MeSH

Related in: MedlinePlus

Behavioral outcome of compound Valerianae Jatamansi Jone during 5 min on the elevated plus-maze. (a) The percentage of time spent in open arms. (b) The percentage of open arms entries. (c) The total number of entries. Bars represent mean ± S.E.M. *P<0.05 or **P<0.01 vs. control group. One way ANOVA with Student-Newman-Keul’s post hoc test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3526556&req=5

Figure 2: Behavioral outcome of compound Valerianae Jatamansi Jone during 5 min on the elevated plus-maze. (a) The percentage of time spent in open arms. (b) The percentage of open arms entries. (c) The total number of entries. Bars represent mean ± S.E.M. *P<0.05 or **P<0.01 vs. control group. One way ANOVA with Student-Newman-Keul’s post hoc test.

Mentions: A one-way ANOVA indicated significant differences among the groups in the time spent in the open arms of the elevated plus maze (F4,72=7.45, P<0.01; Figure 2a) and the percentage of open-arm entries (F4,72=16.70, P < 0.01; Figure 2b). Compound Valeriana jatamansi Jones at concentrations of 2.4 and 4.8 g/kg significantly increased the percentage of time spent in the open arms and the number of entries into the open arm, compared to the saline-treated group (P < 0.05). No differences were observed in the total arm entries among all groups (F4,72=0.89, P>0.05; Figure 2c). After the mice treated with compound Valeriana jatamansi Jones were co-administered flumazenil, there were no differences in the percentage of time spent in the open arms (F4,72=0.49, P>0.05; Figure 3a) or the number of entries into the open arm (F4,72=0.84, P>0.05; Figure 3b) among all groups.


Evaluation of anxiolytic activity of compound Valeriana jatamansi Jones in mice.

You JS, Peng M, Shi JL, Zheng HZ, Liu Y, Zhao BS, Guo JY - BMC Complement Altern Med (2012)

Behavioral outcome of compound Valerianae Jatamansi Jone during 5 min on the elevated plus-maze. (a) The percentage of time spent in open arms. (b) The percentage of open arms entries. (c) The total number of entries. Bars represent mean ± S.E.M. *P<0.05 or **P<0.01 vs. control group. One way ANOVA with Student-Newman-Keul’s post hoc test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3526556&req=5

Figure 2: Behavioral outcome of compound Valerianae Jatamansi Jone during 5 min on the elevated plus-maze. (a) The percentage of time spent in open arms. (b) The percentage of open arms entries. (c) The total number of entries. Bars represent mean ± S.E.M. *P<0.05 or **P<0.01 vs. control group. One way ANOVA with Student-Newman-Keul’s post hoc test.
Mentions: A one-way ANOVA indicated significant differences among the groups in the time spent in the open arms of the elevated plus maze (F4,72=7.45, P<0.01; Figure 2a) and the percentage of open-arm entries (F4,72=16.70, P < 0.01; Figure 2b). Compound Valeriana jatamansi Jones at concentrations of 2.4 and 4.8 g/kg significantly increased the percentage of time spent in the open arms and the number of entries into the open arm, compared to the saline-treated group (P < 0.05). No differences were observed in the total arm entries among all groups (F4,72=0.89, P>0.05; Figure 2c). After the mice treated with compound Valeriana jatamansi Jones were co-administered flumazenil, there were no differences in the percentage of time spent in the open arms (F4,72=0.49, P>0.05; Figure 3a) or the number of entries into the open arm (F4,72=0.84, P>0.05; Figure 3b) among all groups.

Bottom Line: The effects of the compound on spontaneous activity were evaluated by locomotor activity test.We further investigated the mechanism of action underlying the anxiolytic-like effect of compound by pre-treating animals with antagonists of benzodiazepine (flumazenil, 3mg/kg) prior to evaluation using EPM and LDB.However, the anxiolytic-like effects of compound were significantly reduced by pre-treatment with flumazenil (P>0.05).

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, PR China.

ABSTRACT

Background: Compound Valeriana jatamansi Jones is a formula for treating anxiety-related diseases in the clinic, which is composed of Valeriana jatamansi Rhizoma et Radix, Ziziphi Spinosae Semen, Albiziae Cortex and Junci Medulla. The purpose of this study was to explore the anxiolytic properties of this compound in mice.

Methods: Male ICR mice were treated with compound Valerianae Jatamansi Jones (1.2 g/kg, 2.4 g/kg, 4.8 g/kg), saline, diazepam (2 mg/kg) orally for 10 days and then exposed to elevated maze-plus (EPM) and light-dark box (LDB). The effects of the compound on spontaneous activity were evaluated by locomotor activity test. We further investigated the mechanism of action underlying the anxiolytic-like effect of compound by pre-treating animals with antagonists of benzodiazepine (flumazenil, 3mg/kg) prior to evaluation using EPM and LDB.

Results: Compound Valerianae Jatamansi Jones (2.4, 4.8 g/kg, p.o.) significantly increased entries (P<0.05) into and time spent (P<0.05) on the open arms of the EPM, and number of transitions (P<0.05) and time spent (P<0.05) in the light compartment of the LDB. However, the anxiolytic-like effects of compound were significantly reduced by pre-treatment with flumazenil (P>0.05). In addition, compound Valerianae Jatamansi Jones treatment didn't affect the spontaneous activity in mice (P> 0.05).

Conclusions: The present study supports the hypothesis that compound Valeriana jatamansi Jones exert anxiolytic action but no sedative effects in mice and that this effect might be mediated by benzodiazepine receptors.

Show MeSH
Related in: MedlinePlus