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Increased production of hydrogen peroxide by peripheral blood monocytes associated with smoking exposure intensity in smokers.
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In the multiple regression analysis, we identified a positive, statistically significant association between pack-years of smoking and the spontaneous secretion of H2O2 by PBM culture, adjusted for potential confounding variables.The association between PBM culture secretion of H2O2 and the production of TNF-α and IL-6 was not significant.The influence of pack-years smoking may be a key modifiable factor in oxidative stress associated to smoking.
Affiliation: Faculdade de Medicina de Botucatu, Disciplina de Pneumologia, Univ Estadual Paulista, UNESP, Botucatu, São Paulo, Brazil. suzanapneumo@gmail.com.
ABSTRACT
Background: Smoking is known to be associated with oxidative stress; however, it has not been elucidated whether the oxidative response is influenced by the intensity of smoking exposure. Objectives: Evaluate the effect of smoking exposure on the secretion of hydrogen peroxide (H2O2) by the peripheral blood monocytes of smokers. Methods: A total of 25 smokers (50.3±8.8 years, 48% male) underwent the following evaluations: spirometry, pulse oximetry, body composition and total peripheral blood count. Peripheral blood monocyte (PBM) cultures were isolated and maintained, and IL-6 and TNF-α were measured in the plasma and in the supernatants of spontaneous and stimulated cultures. H2O2 was evaluated in the supernatants of the PBM cultures, and a subset of the PBM culture supernatants was stimulated with phorbol myristate acetate (PMA). We also evaluated 38 healthy controls (49.1±8.2 years, 42% male). Results: The spontaneous and stimulated monocytes' secretion of H2O2 were statistically higher in the smokers than in the healthy controls (p<0.001). The H2O2 secretions were statistically significant higher after stimulation with PMA in both groups (p<0.001). In the multiple regression analysis, we identified a positive, statistically significant association between pack-years of smoking and the spontaneous secretion of H2O2 by PBM culture, adjusted for potential confounding variables. The association between PBM culture secretion of H2O2 and the production of TNF-α and IL-6 was not significant. Conclusion: We identified a positive association between higher production of H2O2 in smokers and higher smoking exposure during life. The influence of pack-years smoking may be a key modifiable factor in oxidative stress associated to smoking. No MeSH data available. Related in: MedlinePlus |
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Figure 1: The secretion of hydrogen peroxide, spontaneous and stimulated with phorbol myristate acetate, in smokers and healthy controls. Medians (lines), interquartiles (boxes) and ranges (error bars) are shown. * p<0.001 when comparing controls and smokers. Mentions: The spontaneous and stimulated PBM culture secretions of H2O2 were statistically higher in the smokers than in the healthy controls (p<0.001) (Figure 1). The H2O2 secretion increased significantly after stimulation with PMA in both groups (p<0.001). |
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Affiliation: Faculdade de Medicina de Botucatu, Disciplina de Pneumologia, Univ Estadual Paulista, UNESP, Botucatu, São Paulo, Brazil. suzanapneumo@gmail.com.
Background: Smoking is known to be associated with oxidative stress; however, it has not been elucidated whether the oxidative response is influenced by the intensity of smoking exposure.
Objectives: Evaluate the effect of smoking exposure on the secretion of hydrogen peroxide (H2O2) by the peripheral blood monocytes of smokers.
Methods: A total of 25 smokers (50.3±8.8 years, 48% male) underwent the following evaluations: spirometry, pulse oximetry, body composition and total peripheral blood count. Peripheral blood monocyte (PBM) cultures were isolated and maintained, and IL-6 and TNF-α were measured in the plasma and in the supernatants of spontaneous and stimulated cultures. H2O2 was evaluated in the supernatants of the PBM cultures, and a subset of the PBM culture supernatants was stimulated with phorbol myristate acetate (PMA). We also evaluated 38 healthy controls (49.1±8.2 years, 42% male).
Results: The spontaneous and stimulated monocytes' secretion of H2O2 were statistically higher in the smokers than in the healthy controls (p<0.001). The H2O2 secretions were statistically significant higher after stimulation with PMA in both groups (p<0.001). In the multiple regression analysis, we identified a positive, statistically significant association between pack-years of smoking and the spontaneous secretion of H2O2 by PBM culture, adjusted for potential confounding variables. The association between PBM culture secretion of H2O2 and the production of TNF-α and IL-6 was not significant.
Conclusion: We identified a positive association between higher production of H2O2 in smokers and higher smoking exposure during life. The influence of pack-years smoking may be a key modifiable factor in oxidative stress associated to smoking.
No MeSH data available.
