Limits...
Increased production of hydrogen peroxide by peripheral blood monocytes associated with smoking exposure intensity in smokers.

Tanni SE, Correa CR, Angeleli AY, Vale SA, Coelho LS, Godoy I - J Inflamm (Lond) (2012)

Bottom Line: In the multiple regression analysis, we identified a positive, statistically significant association between pack-years of smoking and the spontaneous secretion of H2O2 by PBM culture, adjusted for potential confounding variables.The association between PBM culture secretion of H2O2 and the production of TNF-α and IL-6 was not significant.The influence of pack-years smoking may be a key modifiable factor in oxidative stress associated to smoking.

View Article: PubMed Central - HTML - PubMed

Affiliation: Faculdade de Medicina de Botucatu, Disciplina de Pneumologia, Univ Estadual Paulista, UNESP, Botucatu, São Paulo, Brazil. suzanapneumo@gmail.com.

ABSTRACT

Background: Smoking is known to be associated with oxidative stress; however, it has not been elucidated whether the oxidative response is influenced by the intensity of smoking exposure.

Objectives: Evaluate the effect of smoking exposure on the secretion of hydrogen peroxide (H2O2) by the peripheral blood monocytes of smokers.

Methods: A total of 25 smokers (50.3±8.8 years, 48% male) underwent the following evaluations: spirometry, pulse oximetry, body composition and total peripheral blood count. Peripheral blood monocyte (PBM) cultures were isolated and maintained, and IL-6 and TNF-α were measured in the plasma and in the supernatants of spontaneous and stimulated cultures. H2O2 was evaluated in the supernatants of the PBM cultures, and a subset of the PBM culture supernatants was stimulated with phorbol myristate acetate (PMA). We also evaluated 38 healthy controls (49.1±8.2 years, 42% male).

Results: The spontaneous and stimulated monocytes' secretion of H2O2 were statistically higher in the smokers than in the healthy controls (p<0.001). The H2O2 secretions were statistically significant higher after stimulation with PMA in both groups (p<0.001). In the multiple regression analysis, we identified a positive, statistically significant association between pack-years of smoking and the spontaneous secretion of H2O2 by PBM culture, adjusted for potential confounding variables. The association between PBM culture secretion of H2O2 and the production of TNF-α and IL-6 was not significant.

Conclusion: We identified a positive association between higher production of H2O2 in smokers and higher smoking exposure during life. The influence of pack-years smoking may be a key modifiable factor in oxidative stress associated to smoking.

No MeSH data available.


Related in: MedlinePlus

The secretion of hydrogen peroxide, spontaneous and stimulated with phorbol myristate acetate, in smokers and healthy controls. Medians (lines), interquartiles (boxes) and ranges (error bars) are shown. * p<0.001 when comparing controls and smokers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3526447&req=5

Figure 1: The secretion of hydrogen peroxide, spontaneous and stimulated with phorbol myristate acetate, in smokers and healthy controls. Medians (lines), interquartiles (boxes) and ranges (error bars) are shown. * p<0.001 when comparing controls and smokers.

Mentions: The spontaneous and stimulated PBM culture secretions of H2O2 were statistically higher in the smokers than in the healthy controls (p<0.001) (Figure 1). The H2O2 secretion increased significantly after stimulation with PMA in both groups (p<0.001).


Increased production of hydrogen peroxide by peripheral blood monocytes associated with smoking exposure intensity in smokers.

Tanni SE, Correa CR, Angeleli AY, Vale SA, Coelho LS, Godoy I - J Inflamm (Lond) (2012)

The secretion of hydrogen peroxide, spontaneous and stimulated with phorbol myristate acetate, in smokers and healthy controls. Medians (lines), interquartiles (boxes) and ranges (error bars) are shown. * p<0.001 when comparing controls and smokers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3526447&req=5

Figure 1: The secretion of hydrogen peroxide, spontaneous and stimulated with phorbol myristate acetate, in smokers and healthy controls. Medians (lines), interquartiles (boxes) and ranges (error bars) are shown. * p<0.001 when comparing controls and smokers.
Mentions: The spontaneous and stimulated PBM culture secretions of H2O2 were statistically higher in the smokers than in the healthy controls (p<0.001) (Figure 1). The H2O2 secretion increased significantly after stimulation with PMA in both groups (p<0.001).

Bottom Line: In the multiple regression analysis, we identified a positive, statistically significant association between pack-years of smoking and the spontaneous secretion of H2O2 by PBM culture, adjusted for potential confounding variables.The association between PBM culture secretion of H2O2 and the production of TNF-α and IL-6 was not significant.The influence of pack-years smoking may be a key modifiable factor in oxidative stress associated to smoking.

View Article: PubMed Central - HTML - PubMed

Affiliation: Faculdade de Medicina de Botucatu, Disciplina de Pneumologia, Univ Estadual Paulista, UNESP, Botucatu, São Paulo, Brazil. suzanapneumo@gmail.com.

ABSTRACT

Background: Smoking is known to be associated with oxidative stress; however, it has not been elucidated whether the oxidative response is influenced by the intensity of smoking exposure.

Objectives: Evaluate the effect of smoking exposure on the secretion of hydrogen peroxide (H2O2) by the peripheral blood monocytes of smokers.

Methods: A total of 25 smokers (50.3±8.8 years, 48% male) underwent the following evaluations: spirometry, pulse oximetry, body composition and total peripheral blood count. Peripheral blood monocyte (PBM) cultures were isolated and maintained, and IL-6 and TNF-α were measured in the plasma and in the supernatants of spontaneous and stimulated cultures. H2O2 was evaluated in the supernatants of the PBM cultures, and a subset of the PBM culture supernatants was stimulated with phorbol myristate acetate (PMA). We also evaluated 38 healthy controls (49.1±8.2 years, 42% male).

Results: The spontaneous and stimulated monocytes' secretion of H2O2 were statistically higher in the smokers than in the healthy controls (p<0.001). The H2O2 secretions were statistically significant higher after stimulation with PMA in both groups (p<0.001). In the multiple regression analysis, we identified a positive, statistically significant association between pack-years of smoking and the spontaneous secretion of H2O2 by PBM culture, adjusted for potential confounding variables. The association between PBM culture secretion of H2O2 and the production of TNF-α and IL-6 was not significant.

Conclusion: We identified a positive association between higher production of H2O2 in smokers and higher smoking exposure during life. The influence of pack-years smoking may be a key modifiable factor in oxidative stress associated to smoking.

No MeSH data available.


Related in: MedlinePlus