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Adhesion GPCRs are widely expressed throughout the subsections of the gastrointestinal tract.

Badiali L, Cedernaes J, Olszewski PK, Nylander O, Vergoni AV, Schiöth HB - BMC Gastroenterol (2012)

Bottom Line: G protein-coupled receptors (GPCRs) represent one of the largest families of transmembrane receptors and the most common drug target.The distribution of these receptors has not been characterized in detail in the gastrointestinal (GI) tract.Most notably, almost all Group III members were ubiquitously expressed, while the restricted expression was characteristic for the majority of group VII members, hinting at more specific/localized roles for some of these receptors.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neuroscience, Uppsala University, BMC, Uppsala, SE 75124, Sweden.

ABSTRACT

Background: G protein-coupled receptors (GPCRs) represent one of the largest families of transmembrane receptors and the most common drug target. The Adhesion subfamily is the second largest one of GPCRs and its several members are known to mediate neural development and immune system functioning through cell-cell and cell-matrix interactions. The distribution of these receptors has not been characterized in detail in the gastrointestinal (GI) tract. Here we present the first comprehensive anatomical profiling of mRNA expression of all 30 Adhesion GPCRs in the rat GI tract divided into twelve subsegments.

Methods: Using RT-qPCR, we studied the expression of Adhesion GPCRs in the esophagus, the corpus and antrum of the stomach, the proximal and distal parts of the duodenum, ileum, jejunum and colon, and the cecum.

Results: We found that twenty-one Adhesion GPCRs (70%) had a widespread (expressed in five or more segments) or ubiquitous (expressed in eleven or more segments) distribution, seven (23%) were restricted to a few segments of the GI tract and two were not expressed in any segment. Most notably, almost all Group III members were ubiquitously expressed, while the restricted expression was characteristic for the majority of group VII members, hinting at more specific/localized roles for some of these receptors.

Conclusions: Overall, the distribution of Adhesion GPCRs points to their important role in GI tract functioning and defines them as a potentially crucial target for pharmacological interventions.

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Related in: MedlinePlus

GI sectioning approximately indicating the different sections of the rat GI tract used for analysis. The GI tract was divided into twelve segments: the esophagus, the corpus and the antrum of the stomach, the proximal and distal parts of the duodenum, ileum, jejunum and colon, and the cecum.
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Figure 1: GI sectioning approximately indicating the different sections of the rat GI tract used for analysis. The GI tract was divided into twelve segments: the esophagus, the corpus and the antrum of the stomach, the proximal and distal parts of the duodenum, ileum, jejunum and colon, and the cecum.

Mentions: Three male Dark Agouti rats (Scanbur AB, Sweden) weighing approximately 200 grams, were kept under constant conditions (12 h dark/light cycle) at 21°C. The animals were fasted overnight but had free access to water before carrying out the experiment. The following morning the animals were intraperitoneally anesthetized with Na-5-ethyl-1-(1’-methyl-propyl)-2-thiobarbituric acid (Inactin®; 125 mg/kg b. wt). Body temperature was maintained at 37.5 ± 0.5°C through a temperature regulator controlling a heating pad. Thereafter, a tracheotomy was performed and a cannula (PE-200) was inserted to guarantee free airways. Following a midline incision to open the abdominal cavity, the following structures, about 5 to 10 mm in length, were localized and dissected (Figure 1): Distal esophagus (a few mm from the stomach); corpus of the stomach; antrum of the stomach; proximal (1 mm from the pylorus) and distal duodenum (4 cm from the pylorus); proximal (9 cm from the pylorus) and distal jejunum (19 cm from the pylorus); proximal (29 cm from pylorus) and distal ileum (2.5 cm from the ileocecal valve); cecum; proximal (5 cm from ileocecal valve) and distal colon (12 cm from the ileocecal valve). The whole GI tract wall was isolated for the RT-qPCR analysis.


Adhesion GPCRs are widely expressed throughout the subsections of the gastrointestinal tract.

Badiali L, Cedernaes J, Olszewski PK, Nylander O, Vergoni AV, Schiöth HB - BMC Gastroenterol (2012)

GI sectioning approximately indicating the different sections of the rat GI tract used for analysis. The GI tract was divided into twelve segments: the esophagus, the corpus and the antrum of the stomach, the proximal and distal parts of the duodenum, ileum, jejunum and colon, and the cecum.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3526421&req=5

Figure 1: GI sectioning approximately indicating the different sections of the rat GI tract used for analysis. The GI tract was divided into twelve segments: the esophagus, the corpus and the antrum of the stomach, the proximal and distal parts of the duodenum, ileum, jejunum and colon, and the cecum.
Mentions: Three male Dark Agouti rats (Scanbur AB, Sweden) weighing approximately 200 grams, were kept under constant conditions (12 h dark/light cycle) at 21°C. The animals were fasted overnight but had free access to water before carrying out the experiment. The following morning the animals were intraperitoneally anesthetized with Na-5-ethyl-1-(1’-methyl-propyl)-2-thiobarbituric acid (Inactin®; 125 mg/kg b. wt). Body temperature was maintained at 37.5 ± 0.5°C through a temperature regulator controlling a heating pad. Thereafter, a tracheotomy was performed and a cannula (PE-200) was inserted to guarantee free airways. Following a midline incision to open the abdominal cavity, the following structures, about 5 to 10 mm in length, were localized and dissected (Figure 1): Distal esophagus (a few mm from the stomach); corpus of the stomach; antrum of the stomach; proximal (1 mm from the pylorus) and distal duodenum (4 cm from the pylorus); proximal (9 cm from the pylorus) and distal jejunum (19 cm from the pylorus); proximal (29 cm from pylorus) and distal ileum (2.5 cm from the ileocecal valve); cecum; proximal (5 cm from ileocecal valve) and distal colon (12 cm from the ileocecal valve). The whole GI tract wall was isolated for the RT-qPCR analysis.

Bottom Line: G protein-coupled receptors (GPCRs) represent one of the largest families of transmembrane receptors and the most common drug target.The distribution of these receptors has not been characterized in detail in the gastrointestinal (GI) tract.Most notably, almost all Group III members were ubiquitously expressed, while the restricted expression was characteristic for the majority of group VII members, hinting at more specific/localized roles for some of these receptors.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neuroscience, Uppsala University, BMC, Uppsala, SE 75124, Sweden.

ABSTRACT

Background: G protein-coupled receptors (GPCRs) represent one of the largest families of transmembrane receptors and the most common drug target. The Adhesion subfamily is the second largest one of GPCRs and its several members are known to mediate neural development and immune system functioning through cell-cell and cell-matrix interactions. The distribution of these receptors has not been characterized in detail in the gastrointestinal (GI) tract. Here we present the first comprehensive anatomical profiling of mRNA expression of all 30 Adhesion GPCRs in the rat GI tract divided into twelve subsegments.

Methods: Using RT-qPCR, we studied the expression of Adhesion GPCRs in the esophagus, the corpus and antrum of the stomach, the proximal and distal parts of the duodenum, ileum, jejunum and colon, and the cecum.

Results: We found that twenty-one Adhesion GPCRs (70%) had a widespread (expressed in five or more segments) or ubiquitous (expressed in eleven or more segments) distribution, seven (23%) were restricted to a few segments of the GI tract and two were not expressed in any segment. Most notably, almost all Group III members were ubiquitously expressed, while the restricted expression was characteristic for the majority of group VII members, hinting at more specific/localized roles for some of these receptors.

Conclusions: Overall, the distribution of Adhesion GPCRs points to their important role in GI tract functioning and defines them as a potentially crucial target for pharmacological interventions.

Show MeSH
Related in: MedlinePlus