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Gene expression profiling of macrophages: implications for an immunosuppressive effect of dissolucytotic gold ions.

Seifert O, Matussek A, Sjögren F, Geffers R, Anderson CD - J Inflamm (Lond) (2012)

Bottom Line: No significant effect on viability of THP-1 cells was demonstrated.A large number of regulated genes were functionally related to immunomodulation.The data obtained in this study offer new insights into the mode of action of gold ions and suggest for the investigation of effects on other key cells and a possible future role of metallic gold as implants in rheumatoid arthritis or other inflammatory conditions.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Dermatology, Ryhov Hospital, S-55185, Jönköping, Sweden. oliver.seifert@lj.se.

ABSTRACT

Background: Gold salts has previously been used in the treatment of rheumatoid arthritis but have been replaced by biologicals such as TNF-α inhibitors. The mechanisms behind the anti-inflammatory effect of metallic gold ions are still unknown, however, recent data showed that charged gold atoms are released from pure metallic gold implants by macrophages via a dissolucytosis membrane, and that gold ions are taken up by local macrophages, mast cells and to some extent fibroblasts. These findings open the question of possible immunomodulatory effects of metallic gold and motivate efforts on a deeper understanding of the effect of metallic gold on key inflammatory cells as macrophages.

Methods: Human macrophage cells (cell line THP-1) were grown on gold foils and intracellular uptake was analysed by autometallography. The impact of phagocytised gold ions on viability of THP-1 cells was investigated by trypan blue staining and TUNEL assay. The global gene expression profile of THP-1 cells after incorporation of gold ions was studied using microarray analysis comprising approximately 20,000 genes. The gene expression data was confirmed by measurement of secreted proteins.

Results: Autometallography showed intracellular uptake of gold ions into THP-1 cells. No significant effect on viability of THP-1 cells was demonstrated. Our data revealed a unique gene expression signature of dissolucytotic THP-1 cells that had taken up gold ions. A large number of regulated genes were functionally related to immunomodulation. Gold ion uptake induced downregulation of genes involved in rheumatoid arthritis such as hepatocyte growth factor, tenascin-C, inhibitor of DNA binding 1 and 3 and matrix metalloproteinase 13.

Conclusion: The data obtained in this study offer new insights into the mode of action of gold ions and suggest for the investigation of effects on other key cells and a possible future role of metallic gold as implants in rheumatoid arthritis or other inflammatory conditions.

No MeSH data available.


Related in: MedlinePlus

THP-1 cells grown on gold foils showed significant decreased expression of early growth response 1 (EGR1) protein in cell culture supernatants compared with control cells after 4 days (n = 3, p < 0.05).
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Figure 3: THP-1 cells grown on gold foils showed significant decreased expression of early growth response 1 (EGR1) protein in cell culture supernatants compared with control cells after 4 days (n = 3, p < 0.05).

Mentions: To determine the effect of gold phagocytosis on the production of three selected secreted proteins specific ELISA’s of cell culture supernatants were performed. Our results showed significant decreased expression of EGR1, LTB and FADS1 (Figure 3,4,5) after 4 days in cells that incorporated gold compared to control cells.


Gene expression profiling of macrophages: implications for an immunosuppressive effect of dissolucytotic gold ions.

Seifert O, Matussek A, Sjögren F, Geffers R, Anderson CD - J Inflamm (Lond) (2012)

THP-1 cells grown on gold foils showed significant decreased expression of early growth response 1 (EGR1) protein in cell culture supernatants compared with control cells after 4 days (n = 3, p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3526405&req=5

Figure 3: THP-1 cells grown on gold foils showed significant decreased expression of early growth response 1 (EGR1) protein in cell culture supernatants compared with control cells after 4 days (n = 3, p < 0.05).
Mentions: To determine the effect of gold phagocytosis on the production of three selected secreted proteins specific ELISA’s of cell culture supernatants were performed. Our results showed significant decreased expression of EGR1, LTB and FADS1 (Figure 3,4,5) after 4 days in cells that incorporated gold compared to control cells.

Bottom Line: No significant effect on viability of THP-1 cells was demonstrated.A large number of regulated genes were functionally related to immunomodulation.The data obtained in this study offer new insights into the mode of action of gold ions and suggest for the investigation of effects on other key cells and a possible future role of metallic gold as implants in rheumatoid arthritis or other inflammatory conditions.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Dermatology, Ryhov Hospital, S-55185, Jönköping, Sweden. oliver.seifert@lj.se.

ABSTRACT

Background: Gold salts has previously been used in the treatment of rheumatoid arthritis but have been replaced by biologicals such as TNF-α inhibitors. The mechanisms behind the anti-inflammatory effect of metallic gold ions are still unknown, however, recent data showed that charged gold atoms are released from pure metallic gold implants by macrophages via a dissolucytosis membrane, and that gold ions are taken up by local macrophages, mast cells and to some extent fibroblasts. These findings open the question of possible immunomodulatory effects of metallic gold and motivate efforts on a deeper understanding of the effect of metallic gold on key inflammatory cells as macrophages.

Methods: Human macrophage cells (cell line THP-1) were grown on gold foils and intracellular uptake was analysed by autometallography. The impact of phagocytised gold ions on viability of THP-1 cells was investigated by trypan blue staining and TUNEL assay. The global gene expression profile of THP-1 cells after incorporation of gold ions was studied using microarray analysis comprising approximately 20,000 genes. The gene expression data was confirmed by measurement of secreted proteins.

Results: Autometallography showed intracellular uptake of gold ions into THP-1 cells. No significant effect on viability of THP-1 cells was demonstrated. Our data revealed a unique gene expression signature of dissolucytotic THP-1 cells that had taken up gold ions. A large number of regulated genes were functionally related to immunomodulation. Gold ion uptake induced downregulation of genes involved in rheumatoid arthritis such as hepatocyte growth factor, tenascin-C, inhibitor of DNA binding 1 and 3 and matrix metalloproteinase 13.

Conclusion: The data obtained in this study offer new insights into the mode of action of gold ions and suggest for the investigation of effects on other key cells and a possible future role of metallic gold as implants in rheumatoid arthritis or other inflammatory conditions.

No MeSH data available.


Related in: MedlinePlus