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Topical application of herbal mixture extract inhibits ovalbumin- or 2,4-dinitrochlorobenzene-induced atopic dermatitis.

Kim SR, Choi HS, Seo HS, Choi YK, Shin YC, Ko SG - Evid Based Complement Alternat Med (2012)

Bottom Line: Atopic dermatitis (AD) is an inflammatory skin disease associated with enhanced T-helper2 (Th2) lymphocyte response to allergens that results in elevated serum eosinophil and Immunoglobulin E (IgE) levels and leukocyte infiltration in atopic skin sites.Histological analyses demonstrated decreased mast cell count as well as dermal infiltration by inflammatory cells.Taken together, our results showed that topical application of KM110329 extracts exerts beneficial effects in AD symptoms, suggesting that KM110329 might be a useful candidate for the treatment of AD.

View Article: PubMed Central - PubMed

Affiliation: Department of Preventive Medicine, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.

ABSTRACT
KM110329 is four traditional herbal medicine mixtures with anti-inflammatory properties. Atopic dermatitis (AD) is an inflammatory skin disease associated with enhanced T-helper2 (Th2) lymphocyte response to allergens that results in elevated serum eosinophil and Immunoglobulin E (IgE) levels and leukocyte infiltration in atopic skin sites. In this study, we investigated the effect of topical application of KM110329 ethanol extract on the ovalbumin (OVA) or 2,4-dinitrochlorobenzene- (DNCB-) induced AD mouse models. For that purpose, we observed the effects of KM110329 on blood eosinophils, skin mast cells, production of serum IgE, and expression of cytokine mRNA in the atopic dermatitis skin lesions of OVA allergen- or DNCB-treated BALB/c mice. KM110329 significantly reduced blood eosinophils cell numbers in OVA or DNCB-treated BALB/c mice. Histological analyses demonstrated decreased mast cell count as well as dermal infiltration by inflammatory cells. In the skin lesions, mRNA expression of interleukine (IL)-4, IL-13, and IL-17 was inhibited by KM110329. KM110329 also suppressed the production of serum IgE level in both the OVA- and DNCB-induced atopic dermatitis model. Taken together, our results showed that topical application of KM110329 extracts exerts beneficial effects in AD symptoms, suggesting that KM110329 might be a useful candidate for the treatment of AD.

No MeSH data available.


Related in: MedlinePlus

Histological features of AD-like skin lesions treated with KM110329. The skin sections were stained with hematoxylin and eosin. Inflammatory cells (arrows) infiltration into the dermis was measured after treatment with KM110329 in the presence of OVA or DNCB. Sections were evaluated using microscope at an original magnification of 400x.
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fig3: Histological features of AD-like skin lesions treated with KM110329. The skin sections were stained with hematoxylin and eosin. Inflammatory cells (arrows) infiltration into the dermis was measured after treatment with KM110329 in the presence of OVA or DNCB. Sections were evaluated using microscope at an original magnification of 400x.

Mentions: To determine whether KM110329 decreases infiltration of inflammatory cells into AD skin lesions, we performed H & E staining on the skin after topical administration of drugs. We observed infiltration of inflammatory cells into both the epidermis and dermis in control group (OVA, DNCB). Whereas, KM110329 decreased such infiltration of inflammatory cells into the skin (Figure 3). Next, we also performed toluidine blue staining for mast cell observation. Repeated cutaneous application of OVA and DNCB increased dermal mast cell number. However, this feature was significantly suppressed by KM110329 compared with control mice (Figure 4). In addition, OVA and DNCB increased numbers of CD3 + (Total T cells) while KM110329 decreased them in epidermal layer (Figure 5).


Topical application of herbal mixture extract inhibits ovalbumin- or 2,4-dinitrochlorobenzene-induced atopic dermatitis.

Kim SR, Choi HS, Seo HS, Choi YK, Shin YC, Ko SG - Evid Based Complement Alternat Med (2012)

Histological features of AD-like skin lesions treated with KM110329. The skin sections were stained with hematoxylin and eosin. Inflammatory cells (arrows) infiltration into the dermis was measured after treatment with KM110329 in the presence of OVA or DNCB. Sections were evaluated using microscope at an original magnification of 400x.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3526256&req=5

fig3: Histological features of AD-like skin lesions treated with KM110329. The skin sections were stained with hematoxylin and eosin. Inflammatory cells (arrows) infiltration into the dermis was measured after treatment with KM110329 in the presence of OVA or DNCB. Sections were evaluated using microscope at an original magnification of 400x.
Mentions: To determine whether KM110329 decreases infiltration of inflammatory cells into AD skin lesions, we performed H & E staining on the skin after topical administration of drugs. We observed infiltration of inflammatory cells into both the epidermis and dermis in control group (OVA, DNCB). Whereas, KM110329 decreased such infiltration of inflammatory cells into the skin (Figure 3). Next, we also performed toluidine blue staining for mast cell observation. Repeated cutaneous application of OVA and DNCB increased dermal mast cell number. However, this feature was significantly suppressed by KM110329 compared with control mice (Figure 4). In addition, OVA and DNCB increased numbers of CD3 + (Total T cells) while KM110329 decreased them in epidermal layer (Figure 5).

Bottom Line: Atopic dermatitis (AD) is an inflammatory skin disease associated with enhanced T-helper2 (Th2) lymphocyte response to allergens that results in elevated serum eosinophil and Immunoglobulin E (IgE) levels and leukocyte infiltration in atopic skin sites.Histological analyses demonstrated decreased mast cell count as well as dermal infiltration by inflammatory cells.Taken together, our results showed that topical application of KM110329 extracts exerts beneficial effects in AD symptoms, suggesting that KM110329 might be a useful candidate for the treatment of AD.

View Article: PubMed Central - PubMed

Affiliation: Department of Preventive Medicine, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.

ABSTRACT
KM110329 is four traditional herbal medicine mixtures with anti-inflammatory properties. Atopic dermatitis (AD) is an inflammatory skin disease associated with enhanced T-helper2 (Th2) lymphocyte response to allergens that results in elevated serum eosinophil and Immunoglobulin E (IgE) levels and leukocyte infiltration in atopic skin sites. In this study, we investigated the effect of topical application of KM110329 ethanol extract on the ovalbumin (OVA) or 2,4-dinitrochlorobenzene- (DNCB-) induced AD mouse models. For that purpose, we observed the effects of KM110329 on blood eosinophils, skin mast cells, production of serum IgE, and expression of cytokine mRNA in the atopic dermatitis skin lesions of OVA allergen- or DNCB-treated BALB/c mice. KM110329 significantly reduced blood eosinophils cell numbers in OVA or DNCB-treated BALB/c mice. Histological analyses demonstrated decreased mast cell count as well as dermal infiltration by inflammatory cells. In the skin lesions, mRNA expression of interleukine (IL)-4, IL-13, and IL-17 was inhibited by KM110329. KM110329 also suppressed the production of serum IgE level in both the OVA- and DNCB-induced atopic dermatitis model. Taken together, our results showed that topical application of KM110329 extracts exerts beneficial effects in AD symptoms, suggesting that KM110329 might be a useful candidate for the treatment of AD.

No MeSH data available.


Related in: MedlinePlus