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Protection of trigonelline on experimental diabetic peripheral neuropathy.

Zhou JY, Zhou SW - Evid Based Complement Alternat Med (2012)

Bottom Line: The mechanisms leading to diabetic peripheral neuropathy are complex and there is no effective drug to treat it.As an active component of several traditional Chinese medicines, trigonelline has beneficial effects on diabetes with hyperlipidemia.These findings suggest that trigonelline has beneficial effects for diabetic peripheral neuropathy through glucagon-like peptide-1 receptor/p38 mitogen-activated protein kinases signaling pathway, nerve conduction velocity, antioxidant enzyme activity, improving micropathological changes of sciatic nerve and decreasing lipid peroxidation.

View Article: PubMed Central - PubMed

Affiliation: Base for Drug Clinical Trial, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.

ABSTRACT
The mechanisms leading to diabetic peripheral neuropathy are complex and there is no effective drug to treat it. As an active component of several traditional Chinese medicines, trigonelline has beneficial effects on diabetes with hyperlipidemia. The protective effects and the mechanism of trigonelline on diabetic peripheral neuropathy were evaluated in streptozotocin- and high-carbohydrate/high-fat diet-induced diabetic rats. Rats were divided into four groups at the end of week 2: control, diabetes, diabetes + trigonelline (40 mg/kg), and diabetes + sitagliptin (4 mg/kg). After 48-week treatment, technologies of nerve conduction, cold and hot immersion test, transmission electron microscopy, real-time PCR, and Western blotting were applied. Serum glucose, serum insulin, insulin sensitivity index, lipid parameters, body weight, sciatic nerve conduction velocity, nociception, glucagon-like peptide-1 receptor mRNA and protein, total and phosphorylated p38 mitogen-activated protein kinases protein expression, malonaldehyde content, and superoxide dismutase activity were altered in diabetic rats, and were near control levels treated with trigonelline. Slight micropathological changes existed in sciatic nerve of trigonelline-treated diabetic rats. These findings suggest that trigonelline has beneficial effects for diabetic peripheral neuropathy through glucagon-like peptide-1 receptor/p38 mitogen-activated protein kinases signaling pathway, nerve conduction velocity, antioxidant enzyme activity, improving micropathological changes of sciatic nerve and decreasing lipid peroxidation.

No MeSH data available.


Related in: MedlinePlus

Effects of trigonelline on GLP-1R protein expression in sciatic nerve. Densitometric analysis of the bands is expressed as integrated optical density (IOD), corrected for the corresponding β-actin. C, control rats; D, diabetic rats; T, trigonelline-treated diabetes; S sitagliptin-treated diabetes. Data are given as mean ± SD (n = 10). *P < 0.01 versus control rats; #P < 0.01 versus diabetes rats.
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fig3: Effects of trigonelline on GLP-1R protein expression in sciatic nerve. Densitometric analysis of the bands is expressed as integrated optical density (IOD), corrected for the corresponding β-actin. C, control rats; D, diabetic rats; T, trigonelline-treated diabetes; S sitagliptin-treated diabetes. Data are given as mean ± SD (n = 10). *P < 0.01 versus control rats; #P < 0.01 versus diabetes rats.

Mentions: GLP-1 level in serum and GLP-1R mRNA and protein expression in sciatic nerve were all significantly decreased in diabetic rats. Forty-eight-week treatment with trigonelline and sitagliptin significantly increased GLP-1 level in diabetic serum to near that of control rats. Trigonelline and sitagliptin significantly increased the downregulated GLP-1R mRNA and protein expression in diabetic sciatic nerve to near those of control rats (Table 3 and Figures 3(a) and 3(b)).


Protection of trigonelline on experimental diabetic peripheral neuropathy.

Zhou JY, Zhou SW - Evid Based Complement Alternat Med (2012)

Effects of trigonelline on GLP-1R protein expression in sciatic nerve. Densitometric analysis of the bands is expressed as integrated optical density (IOD), corrected for the corresponding β-actin. C, control rats; D, diabetic rats; T, trigonelline-treated diabetes; S sitagliptin-treated diabetes. Data are given as mean ± SD (n = 10). *P < 0.01 versus control rats; #P < 0.01 versus diabetes rats.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3526196&req=5

fig3: Effects of trigonelline on GLP-1R protein expression in sciatic nerve. Densitometric analysis of the bands is expressed as integrated optical density (IOD), corrected for the corresponding β-actin. C, control rats; D, diabetic rats; T, trigonelline-treated diabetes; S sitagliptin-treated diabetes. Data are given as mean ± SD (n = 10). *P < 0.01 versus control rats; #P < 0.01 versus diabetes rats.
Mentions: GLP-1 level in serum and GLP-1R mRNA and protein expression in sciatic nerve were all significantly decreased in diabetic rats. Forty-eight-week treatment with trigonelline and sitagliptin significantly increased GLP-1 level in diabetic serum to near that of control rats. Trigonelline and sitagliptin significantly increased the downregulated GLP-1R mRNA and protein expression in diabetic sciatic nerve to near those of control rats (Table 3 and Figures 3(a) and 3(b)).

Bottom Line: The mechanisms leading to diabetic peripheral neuropathy are complex and there is no effective drug to treat it.As an active component of several traditional Chinese medicines, trigonelline has beneficial effects on diabetes with hyperlipidemia.These findings suggest that trigonelline has beneficial effects for diabetic peripheral neuropathy through glucagon-like peptide-1 receptor/p38 mitogen-activated protein kinases signaling pathway, nerve conduction velocity, antioxidant enzyme activity, improving micropathological changes of sciatic nerve and decreasing lipid peroxidation.

View Article: PubMed Central - PubMed

Affiliation: Base for Drug Clinical Trial, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.

ABSTRACT
The mechanisms leading to diabetic peripheral neuropathy are complex and there is no effective drug to treat it. As an active component of several traditional Chinese medicines, trigonelline has beneficial effects on diabetes with hyperlipidemia. The protective effects and the mechanism of trigonelline on diabetic peripheral neuropathy were evaluated in streptozotocin- and high-carbohydrate/high-fat diet-induced diabetic rats. Rats were divided into four groups at the end of week 2: control, diabetes, diabetes + trigonelline (40 mg/kg), and diabetes + sitagliptin (4 mg/kg). After 48-week treatment, technologies of nerve conduction, cold and hot immersion test, transmission electron microscopy, real-time PCR, and Western blotting were applied. Serum glucose, serum insulin, insulin sensitivity index, lipid parameters, body weight, sciatic nerve conduction velocity, nociception, glucagon-like peptide-1 receptor mRNA and protein, total and phosphorylated p38 mitogen-activated protein kinases protein expression, malonaldehyde content, and superoxide dismutase activity were altered in diabetic rats, and were near control levels treated with trigonelline. Slight micropathological changes existed in sciatic nerve of trigonelline-treated diabetic rats. These findings suggest that trigonelline has beneficial effects for diabetic peripheral neuropathy through glucagon-like peptide-1 receptor/p38 mitogen-activated protein kinases signaling pathway, nerve conduction velocity, antioxidant enzyme activity, improving micropathological changes of sciatic nerve and decreasing lipid peroxidation.

No MeSH data available.


Related in: MedlinePlus