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Protection of trigonelline on experimental diabetic peripheral neuropathy.

Zhou JY, Zhou SW - Evid Based Complement Alternat Med (2012)

Bottom Line: The mechanisms leading to diabetic peripheral neuropathy are complex and there is no effective drug to treat it.As an active component of several traditional Chinese medicines, trigonelline has beneficial effects on diabetes with hyperlipidemia.These findings suggest that trigonelline has beneficial effects for diabetic peripheral neuropathy through glucagon-like peptide-1 receptor/p38 mitogen-activated protein kinases signaling pathway, nerve conduction velocity, antioxidant enzyme activity, improving micropathological changes of sciatic nerve and decreasing lipid peroxidation.

View Article: PubMed Central - PubMed

Affiliation: Base for Drug Clinical Trial, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.

ABSTRACT
The mechanisms leading to diabetic peripheral neuropathy are complex and there is no effective drug to treat it. As an active component of several traditional Chinese medicines, trigonelline has beneficial effects on diabetes with hyperlipidemia. The protective effects and the mechanism of trigonelline on diabetic peripheral neuropathy were evaluated in streptozotocin- and high-carbohydrate/high-fat diet-induced diabetic rats. Rats were divided into four groups at the end of week 2: control, diabetes, diabetes + trigonelline (40 mg/kg), and diabetes + sitagliptin (4 mg/kg). After 48-week treatment, technologies of nerve conduction, cold and hot immersion test, transmission electron microscopy, real-time PCR, and Western blotting were applied. Serum glucose, serum insulin, insulin sensitivity index, lipid parameters, body weight, sciatic nerve conduction velocity, nociception, glucagon-like peptide-1 receptor mRNA and protein, total and phosphorylated p38 mitogen-activated protein kinases protein expression, malonaldehyde content, and superoxide dismutase activity were altered in diabetic rats, and were near control levels treated with trigonelline. Slight micropathological changes existed in sciatic nerve of trigonelline-treated diabetic rats. These findings suggest that trigonelline has beneficial effects for diabetic peripheral neuropathy through glucagon-like peptide-1 receptor/p38 mitogen-activated protein kinases signaling pathway, nerve conduction velocity, antioxidant enzyme activity, improving micropathological changes of sciatic nerve and decreasing lipid peroxidation.

No MeSH data available.


Related in: MedlinePlus

Effects of trigonelline on the micromorphology of sciatic nerve. C, control rats; D, diabetic rats; T, trigonelline-treated diabetes; S sitagliptin-treated diabetes.
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fig2: Effects of trigonelline on the micromorphology of sciatic nerve. C, control rats; D, diabetic rats; T, trigonelline-treated diabetes; S sitagliptin-treated diabetes.

Mentions: Ultrastructural examination showed that sciatic nerve of control rats has normal micropathological morphology with integrity structural of nerve fibers and myelin, concentric circle-like arrangement of lamellar myelin, uniform electron density within axons, and normal structure of Schwann cells, mitochondria, and unmyelinated fibers (Figure 2(C)). But diabetic rats showed significant separation of myelinated nerve fiber myelin lamellar, lost layered-structure, disordered arrangement, faded electron density, thinner axons, neurofilament disorder, and swollen mitochondria of Schwann cells (Figure 2(D)). The protective effects of trigonelline were evident with ameliorated micropathology of diabetic sciatic nerve (Figure 2(T)), the same as sitagliptin (Figure 2(S)).


Protection of trigonelline on experimental diabetic peripheral neuropathy.

Zhou JY, Zhou SW - Evid Based Complement Alternat Med (2012)

Effects of trigonelline on the micromorphology of sciatic nerve. C, control rats; D, diabetic rats; T, trigonelline-treated diabetes; S sitagliptin-treated diabetes.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3526196&req=5

fig2: Effects of trigonelline on the micromorphology of sciatic nerve. C, control rats; D, diabetic rats; T, trigonelline-treated diabetes; S sitagliptin-treated diabetes.
Mentions: Ultrastructural examination showed that sciatic nerve of control rats has normal micropathological morphology with integrity structural of nerve fibers and myelin, concentric circle-like arrangement of lamellar myelin, uniform electron density within axons, and normal structure of Schwann cells, mitochondria, and unmyelinated fibers (Figure 2(C)). But diabetic rats showed significant separation of myelinated nerve fiber myelin lamellar, lost layered-structure, disordered arrangement, faded electron density, thinner axons, neurofilament disorder, and swollen mitochondria of Schwann cells (Figure 2(D)). The protective effects of trigonelline were evident with ameliorated micropathology of diabetic sciatic nerve (Figure 2(T)), the same as sitagliptin (Figure 2(S)).

Bottom Line: The mechanisms leading to diabetic peripheral neuropathy are complex and there is no effective drug to treat it.As an active component of several traditional Chinese medicines, trigonelline has beneficial effects on diabetes with hyperlipidemia.These findings suggest that trigonelline has beneficial effects for diabetic peripheral neuropathy through glucagon-like peptide-1 receptor/p38 mitogen-activated protein kinases signaling pathway, nerve conduction velocity, antioxidant enzyme activity, improving micropathological changes of sciatic nerve and decreasing lipid peroxidation.

View Article: PubMed Central - PubMed

Affiliation: Base for Drug Clinical Trial, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.

ABSTRACT
The mechanisms leading to diabetic peripheral neuropathy are complex and there is no effective drug to treat it. As an active component of several traditional Chinese medicines, trigonelline has beneficial effects on diabetes with hyperlipidemia. The protective effects and the mechanism of trigonelline on diabetic peripheral neuropathy were evaluated in streptozotocin- and high-carbohydrate/high-fat diet-induced diabetic rats. Rats were divided into four groups at the end of week 2: control, diabetes, diabetes + trigonelline (40 mg/kg), and diabetes + sitagliptin (4 mg/kg). After 48-week treatment, technologies of nerve conduction, cold and hot immersion test, transmission electron microscopy, real-time PCR, and Western blotting were applied. Serum glucose, serum insulin, insulin sensitivity index, lipid parameters, body weight, sciatic nerve conduction velocity, nociception, glucagon-like peptide-1 receptor mRNA and protein, total and phosphorylated p38 mitogen-activated protein kinases protein expression, malonaldehyde content, and superoxide dismutase activity were altered in diabetic rats, and were near control levels treated with trigonelline. Slight micropathological changes existed in sciatic nerve of trigonelline-treated diabetic rats. These findings suggest that trigonelline has beneficial effects for diabetic peripheral neuropathy through glucagon-like peptide-1 receptor/p38 mitogen-activated protein kinases signaling pathway, nerve conduction velocity, antioxidant enzyme activity, improving micropathological changes of sciatic nerve and decreasing lipid peroxidation.

No MeSH data available.


Related in: MedlinePlus