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Colloidal gold-loaded, biodegradable, polymer-based stavudine nanoparticle uptake by macrophages: an in vitro study.

Basu S, Mukherjee B, Chowdhury SR, Paul P, Choudhury R, Kumar A, Mondal L, Hossain CM, Maji R - Int J Nanomedicine (2012)

Bottom Line: The best formulation was incorporated with fluorescein isothiocyanate.Variations in process parameters, such as speed of homogenization and amount of excipients, affected drug loading and the polydispersity index.The presence of colloidal gold in the particles decreased the drug content and resulted in comparatively faster drug release.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India.

ABSTRACT

Objective: We describe the development, evaluation, and comparison of colloidal gold-loaded, poly(d,l-lactic-co-glycolic acid)-based nanoparticles containing anti-acquired immunodeficiency syndrome drug stavudine and uptake of these nanoparticles by macrophages in vitro.

Methods: WE USED THE FOLLOWING METHODS IN THIS STUDY: drug-excipient interaction by Fourier transform infrared spectroscopy, morphology of nanoparticles by field-emission scanning electron microscopy, particle size by a particle size analyzer, and zeta potential and polydispersity index by a zetasizer. Drug loading and in vitro release were evaluated for formulations. The best formulation was incorporated with fluorescein isothiocyanate. Macrophage uptake of fluorescein isothiocyanate nanoparticles was studied in vitro.

Results: Variations in process parameters, such as speed of homogenization and amount of excipients, affected drug loading and the polydispersity index. We found that the drug was released for a prolonged period (over 63 days) from the nanoparticles, and observed cellular uptake of stavudine nanoparticles by macrophages.

Conclusion: Experimental nanoparticles represent an interesting carrier system for the transport of stavudine to macrophages, providing reduced required drug dose and improved drug delivery to macrophages over an extended period. The presence of colloidal gold in the particles decreased the drug content and resulted in comparatively faster drug release.

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Related in: MedlinePlus

EDX data of colloidal gold nanoparticles alone.Abbreviation: EDX, energy dispersive X-ray spectroscopy.
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f5-ijn-7-6049: EDX data of colloidal gold nanoparticles alone.Abbreviation: EDX, energy dispersive X-ray spectroscopy.

Mentions: Particles were mostly in the nanoscale range with a smooth surface; however, there were some particles larger than nanosize (Figure 2). The Z-average of S1–S5 formulations were 1365 nm, 1136 nm, 211 nm, 201 nm, and 2038 nm, respectively (Table 2); polydispersity indices of the S1–S5 formulations were 0.102, 0.109, 0.424, 0.376, and 0.046, respectively. Particle size distribution (by percentage intensity) of the experimental formulations are shown in Figure 3. Zeta potentials of S1–S5 were −12.6, −8.16, −27.8, −19.6, and −17 mV, respectively (Table 2). The dispersant refractive index, viscosity, and dispersant (water) dielectric constant were 1.33, 0.8872, and 78.5, respectively, at 25°C. The incorporation of gold in nanoparticles was assessed from the EDX data, which showed the presence of gold particles in gold-encapsulated polymeric nanoparticles (Figure 4) as compared to gold nanoparticles (Figure 5) alone.


Colloidal gold-loaded, biodegradable, polymer-based stavudine nanoparticle uptake by macrophages: an in vitro study.

Basu S, Mukherjee B, Chowdhury SR, Paul P, Choudhury R, Kumar A, Mondal L, Hossain CM, Maji R - Int J Nanomedicine (2012)

EDX data of colloidal gold nanoparticles alone.Abbreviation: EDX, energy dispersive X-ray spectroscopy.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3526149&req=5

f5-ijn-7-6049: EDX data of colloidal gold nanoparticles alone.Abbreviation: EDX, energy dispersive X-ray spectroscopy.
Mentions: Particles were mostly in the nanoscale range with a smooth surface; however, there were some particles larger than nanosize (Figure 2). The Z-average of S1–S5 formulations were 1365 nm, 1136 nm, 211 nm, 201 nm, and 2038 nm, respectively (Table 2); polydispersity indices of the S1–S5 formulations were 0.102, 0.109, 0.424, 0.376, and 0.046, respectively. Particle size distribution (by percentage intensity) of the experimental formulations are shown in Figure 3. Zeta potentials of S1–S5 were −12.6, −8.16, −27.8, −19.6, and −17 mV, respectively (Table 2). The dispersant refractive index, viscosity, and dispersant (water) dielectric constant were 1.33, 0.8872, and 78.5, respectively, at 25°C. The incorporation of gold in nanoparticles was assessed from the EDX data, which showed the presence of gold particles in gold-encapsulated polymeric nanoparticles (Figure 4) as compared to gold nanoparticles (Figure 5) alone.

Bottom Line: The best formulation was incorporated with fluorescein isothiocyanate.Variations in process parameters, such as speed of homogenization and amount of excipients, affected drug loading and the polydispersity index.The presence of colloidal gold in the particles decreased the drug content and resulted in comparatively faster drug release.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India.

ABSTRACT

Objective: We describe the development, evaluation, and comparison of colloidal gold-loaded, poly(d,l-lactic-co-glycolic acid)-based nanoparticles containing anti-acquired immunodeficiency syndrome drug stavudine and uptake of these nanoparticles by macrophages in vitro.

Methods: WE USED THE FOLLOWING METHODS IN THIS STUDY: drug-excipient interaction by Fourier transform infrared spectroscopy, morphology of nanoparticles by field-emission scanning electron microscopy, particle size by a particle size analyzer, and zeta potential and polydispersity index by a zetasizer. Drug loading and in vitro release were evaluated for formulations. The best formulation was incorporated with fluorescein isothiocyanate. Macrophage uptake of fluorescein isothiocyanate nanoparticles was studied in vitro.

Results: Variations in process parameters, such as speed of homogenization and amount of excipients, affected drug loading and the polydispersity index. We found that the drug was released for a prolonged period (over 63 days) from the nanoparticles, and observed cellular uptake of stavudine nanoparticles by macrophages.

Conclusion: Experimental nanoparticles represent an interesting carrier system for the transport of stavudine to macrophages, providing reduced required drug dose and improved drug delivery to macrophages over an extended period. The presence of colloidal gold in the particles decreased the drug content and resulted in comparatively faster drug release.

Show MeSH
Related in: MedlinePlus