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Using genetic diversity information to establish core collections of Stylosanthes capitata and Stylosanthes macrocephala.

Santos-Garcia MO, de Toledo-Silva G, Sassaki RP, Ferreira TH, Resende RM, Chiari L, Karia CT, Carvalho MA, Faleiro FG, Zucchi MI, de Souza AP - Genet. Mol. Biol. (2012)

Bottom Line: Nei's genetic diversity was 27% in S. macrocephala and 11% in S. capitata.For S. macrocephala, all of the allelic diversity was represented by 23 accessions, whereas only 13 accessions were necessary to represent all allelic diversity for S. capitata.The data presented herein evidence the population structure present in the Embrapa-Cerrados germplasm collections of S. macrocephala and S. capitata, which may be useful for breeding programs and germplasm conservation.

View Article: PubMed Central - PubMed

Affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, SP, Brazil.

ABSTRACT
Stylosanthes species are important forage legumes in tropical and subtropical areas. S. macrocephala and S. capitata germplasm collections that consist of 134 and 192 accessions, respectively, are maintained at the Brazilian Agricultural Research Corporation Cerrados (Embrapa-Cerrados). Polymorphic microsatellite markers were used to assess genetic diversity and population structure with the aim to assemble a core collection. The mean values of H(O) and H(E) for S. macrocephala were 0.08 and 0.36, respectively, whereas the means for S. capitata were 0.48 and 0.50, respectively. Roger's genetic distance varied from 0 to 0.83 for S. macrocephala and from 0 to 0.85 for S. capitata. Analysis with STRUCTURE software distinguished five groups among the S. macrocephala accessions and four groups among those of S. capitata. Nei's genetic diversity was 27% in S. macrocephala and 11% in S. capitata. Core collections were assembled for both species. For S. macrocephala, all of the allelic diversity was represented by 23 accessions, whereas only 13 accessions were necessary to represent all allelic diversity for S. capitata. The data presented herein evidence the population structure present in the Embrapa-Cerrados germplasm collections of S. macrocephala and S. capitata, which may be useful for breeding programs and germplasm conservation.

No MeSH data available.


Genetic diversity among S. macrocephala accessions. (A) As constructed from the Roger’s dissimilarity matrix using the NJ method. (B) Bar plot representation of the percentage of the gene pool in each S. macrocephala accession.Genetic diversity among S. macrocephala accessions. (A) As constructed from the Roger’s dissimilarity matrix using the NJ method. (B) Bar plot representation of the percentage of the gene pool in each S. macrocephala accession.
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f1-gmb-35-847: Genetic diversity among S. macrocephala accessions. (A) As constructed from the Roger’s dissimilarity matrix using the NJ method. (B) Bar plot representation of the percentage of the gene pool in each S. macrocephala accession.Genetic diversity among S. macrocephala accessions. (A) As constructed from the Roger’s dissimilarity matrix using the NJ method. (B) Bar plot representation of the percentage of the gene pool in each S. macrocephala accession.

Mentions: The method of Evanno et al. (2005) was used to define the maximal ΔK, which was at K = 5 in the S. macrocephala germplasm collection, based on the STRUCTURE analysis (Figure 1). Cluster analysis revealed that 75 of the accessions (57%) were assigned to a single group with more than 80% probability, whereas the other 59 accessions represented a mixture of different groups. Group D comprised the largest number of non-mixed accessions, with 79% of the individuals in this cluster showing more than 80% probability of membership. In contrast, most accessions in groups C and E had less than 80% probability of membership (59% and 62%, respectively). The descriptive data calculated for the individual clusters revealed that HO ranged from 0.03 in group D to 0.14 in group C, and that HE values varied from 0.14 in group D to 0.38 in group C.


Using genetic diversity information to establish core collections of Stylosanthes capitata and Stylosanthes macrocephala.

Santos-Garcia MO, de Toledo-Silva G, Sassaki RP, Ferreira TH, Resende RM, Chiari L, Karia CT, Carvalho MA, Faleiro FG, Zucchi MI, de Souza AP - Genet. Mol. Biol. (2012)

Genetic diversity among S. macrocephala accessions. (A) As constructed from the Roger’s dissimilarity matrix using the NJ method. (B) Bar plot representation of the percentage of the gene pool in each S. macrocephala accession.Genetic diversity among S. macrocephala accessions. (A) As constructed from the Roger’s dissimilarity matrix using the NJ method. (B) Bar plot representation of the percentage of the gene pool in each S. macrocephala accession.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3526094&req=5

f1-gmb-35-847: Genetic diversity among S. macrocephala accessions. (A) As constructed from the Roger’s dissimilarity matrix using the NJ method. (B) Bar plot representation of the percentage of the gene pool in each S. macrocephala accession.Genetic diversity among S. macrocephala accessions. (A) As constructed from the Roger’s dissimilarity matrix using the NJ method. (B) Bar plot representation of the percentage of the gene pool in each S. macrocephala accession.
Mentions: The method of Evanno et al. (2005) was used to define the maximal ΔK, which was at K = 5 in the S. macrocephala germplasm collection, based on the STRUCTURE analysis (Figure 1). Cluster analysis revealed that 75 of the accessions (57%) were assigned to a single group with more than 80% probability, whereas the other 59 accessions represented a mixture of different groups. Group D comprised the largest number of non-mixed accessions, with 79% of the individuals in this cluster showing more than 80% probability of membership. In contrast, most accessions in groups C and E had less than 80% probability of membership (59% and 62%, respectively). The descriptive data calculated for the individual clusters revealed that HO ranged from 0.03 in group D to 0.14 in group C, and that HE values varied from 0.14 in group D to 0.38 in group C.

Bottom Line: Nei's genetic diversity was 27% in S. macrocephala and 11% in S. capitata.For S. macrocephala, all of the allelic diversity was represented by 23 accessions, whereas only 13 accessions were necessary to represent all allelic diversity for S. capitata.The data presented herein evidence the population structure present in the Embrapa-Cerrados germplasm collections of S. macrocephala and S. capitata, which may be useful for breeding programs and germplasm conservation.

View Article: PubMed Central - PubMed

Affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, SP, Brazil.

ABSTRACT
Stylosanthes species are important forage legumes in tropical and subtropical areas. S. macrocephala and S. capitata germplasm collections that consist of 134 and 192 accessions, respectively, are maintained at the Brazilian Agricultural Research Corporation Cerrados (Embrapa-Cerrados). Polymorphic microsatellite markers were used to assess genetic diversity and population structure with the aim to assemble a core collection. The mean values of H(O) and H(E) for S. macrocephala were 0.08 and 0.36, respectively, whereas the means for S. capitata were 0.48 and 0.50, respectively. Roger's genetic distance varied from 0 to 0.83 for S. macrocephala and from 0 to 0.85 for S. capitata. Analysis with STRUCTURE software distinguished five groups among the S. macrocephala accessions and four groups among those of S. capitata. Nei's genetic diversity was 27% in S. macrocephala and 11% in S. capitata. Core collections were assembled for both species. For S. macrocephala, all of the allelic diversity was represented by 23 accessions, whereas only 13 accessions were necessary to represent all allelic diversity for S. capitata. The data presented herein evidence the population structure present in the Embrapa-Cerrados germplasm collections of S. macrocephala and S. capitata, which may be useful for breeding programs and germplasm conservation.

No MeSH data available.