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Genetic characterization of the Neotropical catfish Pimelodus maculatus (Pimelodidae, Siluriformes) in the Upper Uruguay River.

Ribolli J, de Melo CM, Zaniboni-Filho E - Genet. Mol. Biol. (2012)

Bottom Line: Freshwater fish present unique challenges when one attempts to understand the factors that determine the structure of their populations.Habitat fragmentation is a leading cause of population decline that threatens ecosystems worldwide.There was no genetic structure among the four sites analyzed, indicating the existence of only one population group.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia e Cultivo de Peixes de Água Doce, Departamento de Aquicultura, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.

ABSTRACT
Freshwater fish present unique challenges when one attempts to understand the factors that determine the structure of their populations. Habitat fragmentation is a leading cause of population decline that threatens ecosystems worldwide. In this study, we investigated the conservation status of genetic variability in the Neotropical catfish (Pimelodus maculatus). Specifically, we examined the structure and genetic diversity of this species in a region of the Upper Uruguay River fragmented by natural barriers and dams. There was no genetic structure among the four sites analyzed, indicating the existence of only one population group. A combination of environmental management and genetic monitoring should be used to minimize the impact of impoundment on panmitic populations of migratory fish species.

No MeSH data available.


Related in: MedlinePlus

Samples were obtained from four regions in Upper Uruguay River Basin: BG – upstream of the Barra Grande dam (sites 1–3; n = 52), MA –downstream of the Barra Grande dam and upstream of the Machadinho dam (sites 4–5; n = 60), IT – downstream of the Machadinho dam and upstream of the Itá dam (sites 7–9; n = 48) and DI – downstream of the Itá dam (sites 10 and 11; n = 50).
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f1-gmb-35-761: Samples were obtained from four regions in Upper Uruguay River Basin: BG – upstream of the Barra Grande dam (sites 1–3; n = 52), MA –downstream of the Barra Grande dam and upstream of the Machadinho dam (sites 4–5; n = 60), IT – downstream of the Machadinho dam and upstream of the Itá dam (sites 7–9; n = 48) and DI – downstream of the Itá dam (sites 10 and 11; n = 50).

Mentions: Two hundred and ten specimens of P. maculatus were collected from 2007 to 2009 at different locations along the Upper Uruguay River during all seasons (Figure 1). All samples were collected after construction of the dams and were obtained from four sites: BG – upstream of the Barra Grande dam (n = 51), MA – downstream of the Barra Grande dam and upstream of the Machadinho dam (n = 60), IT – downstream of the Machadinho dam and upstream of the Itá dam (n = 48) and DI – downstream of the Itá dam (n = 51) (Table 1). Genomic DNA was extracted from fin clips using the proteinase K/phenol-chloroform protocol (Sambrook et al., 2001).


Genetic characterization of the Neotropical catfish Pimelodus maculatus (Pimelodidae, Siluriformes) in the Upper Uruguay River.

Ribolli J, de Melo CM, Zaniboni-Filho E - Genet. Mol. Biol. (2012)

Samples were obtained from four regions in Upper Uruguay River Basin: BG – upstream of the Barra Grande dam (sites 1–3; n = 52), MA –downstream of the Barra Grande dam and upstream of the Machadinho dam (sites 4–5; n = 60), IT – downstream of the Machadinho dam and upstream of the Itá dam (sites 7–9; n = 48) and DI – downstream of the Itá dam (sites 10 and 11; n = 50).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3526083&req=5

f1-gmb-35-761: Samples were obtained from four regions in Upper Uruguay River Basin: BG – upstream of the Barra Grande dam (sites 1–3; n = 52), MA –downstream of the Barra Grande dam and upstream of the Machadinho dam (sites 4–5; n = 60), IT – downstream of the Machadinho dam and upstream of the Itá dam (sites 7–9; n = 48) and DI – downstream of the Itá dam (sites 10 and 11; n = 50).
Mentions: Two hundred and ten specimens of P. maculatus were collected from 2007 to 2009 at different locations along the Upper Uruguay River during all seasons (Figure 1). All samples were collected after construction of the dams and were obtained from four sites: BG – upstream of the Barra Grande dam (n = 51), MA – downstream of the Barra Grande dam and upstream of the Machadinho dam (n = 60), IT – downstream of the Machadinho dam and upstream of the Itá dam (n = 48) and DI – downstream of the Itá dam (n = 51) (Table 1). Genomic DNA was extracted from fin clips using the proteinase K/phenol-chloroform protocol (Sambrook et al., 2001).

Bottom Line: Freshwater fish present unique challenges when one attempts to understand the factors that determine the structure of their populations.Habitat fragmentation is a leading cause of population decline that threatens ecosystems worldwide.There was no genetic structure among the four sites analyzed, indicating the existence of only one population group.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia e Cultivo de Peixes de Água Doce, Departamento de Aquicultura, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.

ABSTRACT
Freshwater fish present unique challenges when one attempts to understand the factors that determine the structure of their populations. Habitat fragmentation is a leading cause of population decline that threatens ecosystems worldwide. In this study, we investigated the conservation status of genetic variability in the Neotropical catfish (Pimelodus maculatus). Specifically, we examined the structure and genetic diversity of this species in a region of the Upper Uruguay River fragmented by natural barriers and dams. There was no genetic structure among the four sites analyzed, indicating the existence of only one population group. A combination of environmental management and genetic monitoring should be used to minimize the impact of impoundment on panmitic populations of migratory fish species.

No MeSH data available.


Related in: MedlinePlus