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Elemental labelling combined with liquid chromatography inductively coupled plasma mass spectrometry for quantification of biomolecules: a review.

Kretschy D, Koellensperger G, Hann S - Anal. Chim. Acta (2012)

Bottom Line: Fundamental methodology of elemental labelling will be highlighted and analytical, as well as biomedical applications will be presented.A special focus will lie on established applications underlining benefits and bottlenecks of such approaches for the implementation in real life analysis.Key research made in this field will be summarized and a perspective for future developments including sophisticated and innovative applications will given.

View Article: PubMed Central - PubMed

Affiliation: University of Natural Resources and Life Sciences, BOKU Vienna, Department of Chemistry, Division of Analytical Chemistry, Muthgasse 18, A-1190 Vienna, Austria.

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Reaction mechanism of (A) attachment of a protein SH group to a maleimide reactive group and of (B) attachment of a protein NH2 group to an isothiocyanato reactive group via Schiffsche base reaction.
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fig0015: Reaction mechanism of (A) attachment of a protein SH group to a maleimide reactive group and of (B) attachment of a protein NH2 group to an isothiocyanato reactive group via Schiffsche base reaction.

Mentions: Maleimide, iodoacetamide, bromoacetamide, isothiocyanato or azide groups are commonly used, but many other groups can also be employed [101] and are commercially available [www.piercenet.com[102]]. The majority of reactive groups of elemental labels target thiol or amine groups. In Fig. 2 the reaction mechanism of (A) the attachment of a maleimide reactive group to a thiol group of a protein and of (B) the attachment of an iosthiocyanato group to an amine group of a protein is depicted.


Elemental labelling combined with liquid chromatography inductively coupled plasma mass spectrometry for quantification of biomolecules: a review.

Kretschy D, Koellensperger G, Hann S - Anal. Chim. Acta (2012)

Reaction mechanism of (A) attachment of a protein SH group to a maleimide reactive group and of (B) attachment of a protein NH2 group to an isothiocyanato reactive group via Schiffsche base reaction.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475989&req=5

fig0015: Reaction mechanism of (A) attachment of a protein SH group to a maleimide reactive group and of (B) attachment of a protein NH2 group to an isothiocyanato reactive group via Schiffsche base reaction.
Mentions: Maleimide, iodoacetamide, bromoacetamide, isothiocyanato or azide groups are commonly used, but many other groups can also be employed [101] and are commercially available [www.piercenet.com[102]]. The majority of reactive groups of elemental labels target thiol or amine groups. In Fig. 2 the reaction mechanism of (A) the attachment of a maleimide reactive group to a thiol group of a protein and of (B) the attachment of an iosthiocyanato group to an amine group of a protein is depicted.

Bottom Line: Fundamental methodology of elemental labelling will be highlighted and analytical, as well as biomedical applications will be presented.A special focus will lie on established applications underlining benefits and bottlenecks of such approaches for the implementation in real life analysis.Key research made in this field will be summarized and a perspective for future developments including sophisticated and innovative applications will given.

View Article: PubMed Central - PubMed

Affiliation: University of Natural Resources and Life Sciences, BOKU Vienna, Department of Chemistry, Division of Analytical Chemistry, Muthgasse 18, A-1190 Vienna, Austria.

Show MeSH