Elemental labelling combined with liquid chromatography inductively coupled plasma mass spectrometry for quantification of biomolecules: a review.
Bottom Line: Fundamental methodology of elemental labelling will be highlighted and analytical, as well as biomedical applications will be presented.A special focus will lie on established applications underlining benefits and bottlenecks of such approaches for the implementation in real life analysis.Key research made in this field will be summarized and a perspective for future developments including sophisticated and innovative applications will given.
Affiliation: University of Natural Resources and Life Sciences, BOKU Vienna, Department of Chemistry, Division of Analytical Chemistry, Muthgasse 18, A-1190 Vienna, Austria.Show MeSH
Mentions: Maleimide, iodoacetamide, bromoacetamide, isothiocyanato or azide groups are commonly used, but many other groups can also be employed  and are commercially available [www.piercenet.com]. The majority of reactive groups of elemental labels target thiol or amine groups. In Fig. 2 the reaction mechanism of (A) the attachment of a maleimide reactive group to a thiol group of a protein and of (B) the attachment of an iosthiocyanato group to an amine group of a protein is depicted.
Affiliation: University of Natural Resources and Life Sciences, BOKU Vienna, Department of Chemistry, Division of Analytical Chemistry, Muthgasse 18, A-1190 Vienna, Austria.