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Activation of innate anti-viral immune response genes in symptomatic benign prostatic hyperplasia.

Madigan AA, Sobek KM, Cummings JL, Green WR, Bacich DJ, O'Keefe DS - Genes Immun. (2012)

Bottom Line: We determined the expression of genes involved in the innate anti-viral immune response in tissues from patients undergoing surgery to alleviate the symptoms of BPH, and compared the results with prostate tissue with histologic BPH, but from patients with few urinary issues (asymptomatic BPH).We found that expression of complement factor I, apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like protein 3G, oligoadenylate synthetase 2 and interferon-induced tetratricopeptide 1, four genes whose protein products are involved in the innate anti-viral immune response, was significantly transcriptionally upregulated in symptomatic BPH.Additionally, we observe hypomethylation and concomitant expression of ancient retroviral-like sequences, the long interspersed nuclear element 1 retrotransposons, in symptomatic BPH when compared with normal prostate tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15232, USA.

ABSTRACT
Benign prostatic hyperplasia (BPH) is the most common urologic disease in men over age 50. Symptoms include acute urinary retention, urgency to urinate and nocturia. For patients with severe symptoms, surgical treatment is used to remove the affected tissue. Interestingly, the presence of histologic BPH does not always correlate with symptoms. The molecular basis of symptomatic BPH and how it differs from asymptomatic BPH is unknown. Investigation into the molecular players involved in symptomatic BPH will likely give insight into novel therapeutic, and potentially preventative, targets. We determined the expression of genes involved in the innate anti-viral immune response in tissues from patients undergoing surgery to alleviate the symptoms of BPH, and compared the results with prostate tissue with histologic BPH, but from patients with few urinary issues (asymptomatic BPH). We found that expression of complement factor I, apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like protein 3G, oligoadenylate synthetase 2 and interferon-induced tetratricopeptide 1, four genes whose protein products are involved in the innate anti-viral immune response, was significantly transcriptionally upregulated in symptomatic BPH. Additionally, we observe hypomethylation and concomitant expression of ancient retroviral-like sequences, the long interspersed nuclear element 1 retrotransposons, in symptomatic BPH when compared with normal prostate tissue. These findings merit further investigation into the anti-viral immune response in symptomatic BPH.

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a. Correlation between IFIT1 real time PCR results (IFIT1 relative gene expression – IFIT1/GAPDH) and mass in grams of the asymptomatic BPH samples. Statistical analysis was carried out using a Pearson’s correlation, with the Pearson’s r = 0.65 and p = 0.0172. b. Correlation between APOBEC3G and IFIT1 relative gene expression (normalized to GAPDH) in the symptomatic BPH samples. Pearson’s correlation statistical analysis resulted in a Pearson’s r = 0.65 and p = 0.0066.
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Figure 4: a. Correlation between IFIT1 real time PCR results (IFIT1 relative gene expression – IFIT1/GAPDH) and mass in grams of the asymptomatic BPH samples. Statistical analysis was carried out using a Pearson’s correlation, with the Pearson’s r = 0.65 and p = 0.0172. b. Correlation between APOBEC3G and IFIT1 relative gene expression (normalized to GAPDH) in the symptomatic BPH samples. Pearson’s correlation statistical analysis resulted in a Pearson’s r = 0.65 and p = 0.0066.

Mentions: When analyzing our IFIT1 data we observed that the asymptomatic BPH samples with large masses had the highest fold changes in IFIT1 expression. Therefore we wanted to determine if there was a significant correlation between IFIT1 fold change and the mass in grams of the asymptomatic BPH prostates. We found that there is a strong positive correlation between IFIT1 expression and the mass of the asymptomatic BPH prostates (Pearson’s r = 0.65), and this was found to be statistically significant (Figure 4a; p = 0.017).


Activation of innate anti-viral immune response genes in symptomatic benign prostatic hyperplasia.

Madigan AA, Sobek KM, Cummings JL, Green WR, Bacich DJ, O'Keefe DS - Genes Immun. (2012)

a. Correlation between IFIT1 real time PCR results (IFIT1 relative gene expression – IFIT1/GAPDH) and mass in grams of the asymptomatic BPH samples. Statistical analysis was carried out using a Pearson’s correlation, with the Pearson’s r = 0.65 and p = 0.0172. b. Correlation between APOBEC3G and IFIT1 relative gene expression (normalized to GAPDH) in the symptomatic BPH samples. Pearson’s correlation statistical analysis resulted in a Pearson’s r = 0.65 and p = 0.0066.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3475748&req=5

Figure 4: a. Correlation between IFIT1 real time PCR results (IFIT1 relative gene expression – IFIT1/GAPDH) and mass in grams of the asymptomatic BPH samples. Statistical analysis was carried out using a Pearson’s correlation, with the Pearson’s r = 0.65 and p = 0.0172. b. Correlation between APOBEC3G and IFIT1 relative gene expression (normalized to GAPDH) in the symptomatic BPH samples. Pearson’s correlation statistical analysis resulted in a Pearson’s r = 0.65 and p = 0.0066.
Mentions: When analyzing our IFIT1 data we observed that the asymptomatic BPH samples with large masses had the highest fold changes in IFIT1 expression. Therefore we wanted to determine if there was a significant correlation between IFIT1 fold change and the mass in grams of the asymptomatic BPH prostates. We found that there is a strong positive correlation between IFIT1 expression and the mass of the asymptomatic BPH prostates (Pearson’s r = 0.65), and this was found to be statistically significant (Figure 4a; p = 0.017).

Bottom Line: We determined the expression of genes involved in the innate anti-viral immune response in tissues from patients undergoing surgery to alleviate the symptoms of BPH, and compared the results with prostate tissue with histologic BPH, but from patients with few urinary issues (asymptomatic BPH).We found that expression of complement factor I, apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like protein 3G, oligoadenylate synthetase 2 and interferon-induced tetratricopeptide 1, four genes whose protein products are involved in the innate anti-viral immune response, was significantly transcriptionally upregulated in symptomatic BPH.Additionally, we observe hypomethylation and concomitant expression of ancient retroviral-like sequences, the long interspersed nuclear element 1 retrotransposons, in symptomatic BPH when compared with normal prostate tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15232, USA.

ABSTRACT
Benign prostatic hyperplasia (BPH) is the most common urologic disease in men over age 50. Symptoms include acute urinary retention, urgency to urinate and nocturia. For patients with severe symptoms, surgical treatment is used to remove the affected tissue. Interestingly, the presence of histologic BPH does not always correlate with symptoms. The molecular basis of symptomatic BPH and how it differs from asymptomatic BPH is unknown. Investigation into the molecular players involved in symptomatic BPH will likely give insight into novel therapeutic, and potentially preventative, targets. We determined the expression of genes involved in the innate anti-viral immune response in tissues from patients undergoing surgery to alleviate the symptoms of BPH, and compared the results with prostate tissue with histologic BPH, but from patients with few urinary issues (asymptomatic BPH). We found that expression of complement factor I, apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like protein 3G, oligoadenylate synthetase 2 and interferon-induced tetratricopeptide 1, four genes whose protein products are involved in the innate anti-viral immune response, was significantly transcriptionally upregulated in symptomatic BPH. Additionally, we observe hypomethylation and concomitant expression of ancient retroviral-like sequences, the long interspersed nuclear element 1 retrotransposons, in symptomatic BPH when compared with normal prostate tissue. These findings merit further investigation into the anti-viral immune response in symptomatic BPH.

Show MeSH
Related in: MedlinePlus