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"SP-G", a putative new surfactant protein--tissue localization and 3D structure.

Rausch F, Schicht M, Paulsen F, Ngueya I, Bräuer L, Brandt W - PLoS ONE (2012)

Bottom Line: In this work, computational chemistry and molecular-biological methods were combined to localize and characterize SP-G.With the help of a protein structure model, specific antibodies were obtained which allowed the detection of SP-G not only on mRNA but also on protein level.This includes also the possibility of interactions with lipid systems and with that, a potential surface-regulatory feature of SP-G.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Halle, Germany.

ABSTRACT
Surfactant proteins (SP) are well known from human lung. These proteins assist the formation of a monolayer of surface-active phospholipids at the liquid-air interface of the alveolar lining, play a major role in lowering the surface tension of interfaces, and have functions in innate and adaptive immune defense. During recent years it became obvious that SPs are also part of other tissues and fluids such as tear fluid, gingiva, saliva, the nasolacrimal system, and kidney. Recently, a putative new surfactant protein (SFTA2 or SP-G) was identified, which has no sequence or structural identity to the already know surfactant proteins. In this work, computational chemistry and molecular-biological methods were combined to localize and characterize SP-G. With the help of a protein structure model, specific antibodies were obtained which allowed the detection of SP-G not only on mRNA but also on protein level. The localization of this protein in different human tissues, sequence based prediction tools for posttranslational modifications and molecular dynamic simulations reveal that SP-G has physicochemical properties similar to the already known surfactant proteins B and C. This includes also the possibility of interactions with lipid systems and with that, a potential surface-regulatory feature of SP-G. In conclusion, the results indicate SP-G as a new surfactant protein which represents an until now unknown surfactant protein class.

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Related in: MedlinePlus

Immunohistochemical detection of SP-G in human tissue samples.Sections from lung [A-B], eyelid [C], conjunctiva [D], meibomian glands [E], lacrimal gland [F], kidney [G], sebaceous gland [H], and testis [I] were analyzed. Red stained areas within the tissues indicate SP-G expression. Insets in the figures show magnifications for the respective tissue. Control sections (secondary antibody only) were negative (unstained) for each tissue. Scale bars in [A, C, E, I] 50 µm, in [B, D, F, G, H] 100 µm.
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pone-0047789-g008: Immunohistochemical detection of SP-G in human tissue samples.Sections from lung [A-B], eyelid [C], conjunctiva [D], meibomian glands [E], lacrimal gland [F], kidney [G], sebaceous gland [H], and testis [I] were analyzed. Red stained areas within the tissues indicate SP-G expression. Insets in the figures show magnifications for the respective tissue. Control sections (secondary antibody only) were negative (unstained) for each tissue. Scale bars in [A, C, E, I] 50 µm, in [B, D, F, G, H] 100 µm.

Mentions: All investigated tissue samples show antibody reactivity against SP-G (Figure 8A, B, C, D, E). Paraffin-embedded 6-µm sections from lung, eyelid, conjunctiva, meibomian glands, lacrimal gland, kidney, sebaceous gland and testis were analyzed. Control sections (secondary antibody only) were negative (unstained) for each tissue. The insets of the figures show magnifications for the respective tissue. For all tissues, red staining reveals positive antibody reaction for surfactant protein G. Lung: In the lung, SP-G could be detected as a superficial layer of the epithelium of the bronchioles. The alveolar membrane, especially the alveolar cells (I & II) are negative (Figure 8A, B).


"SP-G", a putative new surfactant protein--tissue localization and 3D structure.

Rausch F, Schicht M, Paulsen F, Ngueya I, Bräuer L, Brandt W - PLoS ONE (2012)

Immunohistochemical detection of SP-G in human tissue samples.Sections from lung [A-B], eyelid [C], conjunctiva [D], meibomian glands [E], lacrimal gland [F], kidney [G], sebaceous gland [H], and testis [I] were analyzed. Red stained areas within the tissues indicate SP-G expression. Insets in the figures show magnifications for the respective tissue. Control sections (secondary antibody only) were negative (unstained) for each tissue. Scale bars in [A, C, E, I] 50 µm, in [B, D, F, G, H] 100 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3475697&req=5

pone-0047789-g008: Immunohistochemical detection of SP-G in human tissue samples.Sections from lung [A-B], eyelid [C], conjunctiva [D], meibomian glands [E], lacrimal gland [F], kidney [G], sebaceous gland [H], and testis [I] were analyzed. Red stained areas within the tissues indicate SP-G expression. Insets in the figures show magnifications for the respective tissue. Control sections (secondary antibody only) were negative (unstained) for each tissue. Scale bars in [A, C, E, I] 50 µm, in [B, D, F, G, H] 100 µm.
Mentions: All investigated tissue samples show antibody reactivity against SP-G (Figure 8A, B, C, D, E). Paraffin-embedded 6-µm sections from lung, eyelid, conjunctiva, meibomian glands, lacrimal gland, kidney, sebaceous gland and testis were analyzed. Control sections (secondary antibody only) were negative (unstained) for each tissue. The insets of the figures show magnifications for the respective tissue. For all tissues, red staining reveals positive antibody reaction for surfactant protein G. Lung: In the lung, SP-G could be detected as a superficial layer of the epithelium of the bronchioles. The alveolar membrane, especially the alveolar cells (I & II) are negative (Figure 8A, B).

Bottom Line: In this work, computational chemistry and molecular-biological methods were combined to localize and characterize SP-G.With the help of a protein structure model, specific antibodies were obtained which allowed the detection of SP-G not only on mRNA but also on protein level.This includes also the possibility of interactions with lipid systems and with that, a potential surface-regulatory feature of SP-G.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Halle, Germany.

ABSTRACT
Surfactant proteins (SP) are well known from human lung. These proteins assist the formation of a monolayer of surface-active phospholipids at the liquid-air interface of the alveolar lining, play a major role in lowering the surface tension of interfaces, and have functions in innate and adaptive immune defense. During recent years it became obvious that SPs are also part of other tissues and fluids such as tear fluid, gingiva, saliva, the nasolacrimal system, and kidney. Recently, a putative new surfactant protein (SFTA2 or SP-G) was identified, which has no sequence or structural identity to the already know surfactant proteins. In this work, computational chemistry and molecular-biological methods were combined to localize and characterize SP-G. With the help of a protein structure model, specific antibodies were obtained which allowed the detection of SP-G not only on mRNA but also on protein level. The localization of this protein in different human tissues, sequence based prediction tools for posttranslational modifications and molecular dynamic simulations reveal that SP-G has physicochemical properties similar to the already known surfactant proteins B and C. This includes also the possibility of interactions with lipid systems and with that, a potential surface-regulatory feature of SP-G. In conclusion, the results indicate SP-G as a new surfactant protein which represents an until now unknown surfactant protein class.

Show MeSH
Related in: MedlinePlus