Limits...
MOV10 RNA helicase is a potent inhibitor of retrotransposition in cells.

Goodier JL, Cheung LE, Kazazian HH - PLoS Genet. (2012)

Bottom Line: However, unlike MOV10, these other helicases do not strongly inhibit retrotransposition, an activity dependent upon intact helicase domains.Therefore, the host has evolved defense mechanisms to protect against retrotransposition, an arsenal we are only beginning to understand.With homologs in other vertebrates, insects, and plants, MOV10 may represent an ancient and innate form of immunity against both infective viruses and endogenous retroelements.

View Article: PubMed Central - PubMed

Affiliation: McKusick-Nathans Institute for Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. jgoodier@jhmi.edu

ABSTRACT
MOV10 protein, a putative RNA helicase and component of the RNA-induced silencing complex (RISC), inhibits retrovirus replication. We show that MOV10 also severely restricts human LINE1 (L1), Alu, and SVA retrotransposons. MOV10 associates with the L1 ribonucleoprotein particle, along with other RNA helicases including DDX5, DHX9, DDX17, DDX21, and DDX39A. However, unlike MOV10, these other helicases do not strongly inhibit retrotransposition, an activity dependent upon intact helicase domains. MOV10 association with retrotransposons is further supported by its colocalization with L1 ORF1 protein in stress granules, by cytoplasmic structures associated with RNA silencing, and by the ability of MOV10 to reduce endogenous and ectopic L1 expression. The majority of the human genome is repetitive DNA, most of which is the detritus of millions of years of accumulated retrotransposition. Retrotransposons remain active mutagens, and their insertion can disrupt gene function. Therefore, the host has evolved defense mechanisms to protect against retrotransposition, an arsenal we are only beginning to understand. With homologs in other vertebrates, insects, and plants, MOV10 may represent an ancient and innate form of immunity against both infective viruses and endogenous retroelements.

Show MeSH

Related in: MedlinePlus

ATP-dependent RNA helicases other than MOV10 also associate with L1 RNPs expressed from pc-L1-1FH.In α-FLAG immunoprecipitates, only association with myc-tagged full-length DDX17 (iso1) is resistant to RNase digestion.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3475670&req=5

pgen-1002941-g004: ATP-dependent RNA helicases other than MOV10 also associate with L1 RNPs expressed from pc-L1-1FH.In α-FLAG immunoprecipitates, only association with myc-tagged full-length DDX17 (iso1) is resistant to RNase digestion.

Mentions: Like MOV10, V5 epitope-tagged DDX5, DHX9, DDX21, and DDX39A proteins co-IPed with tagged L1 RNP complexes in an RNA-dependent manner (Figure 4). Only myc-tagged DDX17 resisted RNase treatment, predicting a tight association with the L1 RNP or direct binding to ORF1p. We failed in attempts to coimmunoprecipitate FLAG-tagged mouse MOV10L protein together with L1-RP tagged with a tandem affinity purification (TAP) tag on ORF1p (not shown).


MOV10 RNA helicase is a potent inhibitor of retrotransposition in cells.

Goodier JL, Cheung LE, Kazazian HH - PLoS Genet. (2012)

ATP-dependent RNA helicases other than MOV10 also associate with L1 RNPs expressed from pc-L1-1FH.In α-FLAG immunoprecipitates, only association with myc-tagged full-length DDX17 (iso1) is resistant to RNase digestion.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475670&req=5

pgen-1002941-g004: ATP-dependent RNA helicases other than MOV10 also associate with L1 RNPs expressed from pc-L1-1FH.In α-FLAG immunoprecipitates, only association with myc-tagged full-length DDX17 (iso1) is resistant to RNase digestion.
Mentions: Like MOV10, V5 epitope-tagged DDX5, DHX9, DDX21, and DDX39A proteins co-IPed with tagged L1 RNP complexes in an RNA-dependent manner (Figure 4). Only myc-tagged DDX17 resisted RNase treatment, predicting a tight association with the L1 RNP or direct binding to ORF1p. We failed in attempts to coimmunoprecipitate FLAG-tagged mouse MOV10L protein together with L1-RP tagged with a tandem affinity purification (TAP) tag on ORF1p (not shown).

Bottom Line: However, unlike MOV10, these other helicases do not strongly inhibit retrotransposition, an activity dependent upon intact helicase domains.Therefore, the host has evolved defense mechanisms to protect against retrotransposition, an arsenal we are only beginning to understand.With homologs in other vertebrates, insects, and plants, MOV10 may represent an ancient and innate form of immunity against both infective viruses and endogenous retroelements.

View Article: PubMed Central - PubMed

Affiliation: McKusick-Nathans Institute for Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. jgoodier@jhmi.edu

ABSTRACT
MOV10 protein, a putative RNA helicase and component of the RNA-induced silencing complex (RISC), inhibits retrovirus replication. We show that MOV10 also severely restricts human LINE1 (L1), Alu, and SVA retrotransposons. MOV10 associates with the L1 ribonucleoprotein particle, along with other RNA helicases including DDX5, DHX9, DDX17, DDX21, and DDX39A. However, unlike MOV10, these other helicases do not strongly inhibit retrotransposition, an activity dependent upon intact helicase domains. MOV10 association with retrotransposons is further supported by its colocalization with L1 ORF1 protein in stress granules, by cytoplasmic structures associated with RNA silencing, and by the ability of MOV10 to reduce endogenous and ectopic L1 expression. The majority of the human genome is repetitive DNA, most of which is the detritus of millions of years of accumulated retrotransposition. Retrotransposons remain active mutagens, and their insertion can disrupt gene function. Therefore, the host has evolved defense mechanisms to protect against retrotransposition, an arsenal we are only beginning to understand. With homologs in other vertebrates, insects, and plants, MOV10 may represent an ancient and innate form of immunity against both infective viruses and endogenous retroelements.

Show MeSH
Related in: MedlinePlus