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Escherichia coli-mediated impairment of ureteric contractility is uropathogenic E. coli specific.

Floyd RV, Upton M, Hultgren SJ, Wray S, Burdyga TV, Winstanley C - J. Infect. Dis. (2012)

Bottom Line: Non-UPEC had no significant effect on contractility, with a mean decrease after 8 hours of 8.8%, compared with 8.8% in controls.UPEC effects on contractility were strain specific, with decreases from 9.47% to 96.7%.We find that (1) non-UPEC do not affect ureteric contractility, (2) impairment of contractility is a common feature of UPEC, and (3) the mechanism varies between strains, but for the most potent UPEC type 1 fimbriae are involved.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, United Kingdom. floyd78@liv.ac.uk

ABSTRACT

Background: Ureters are fundamental for keeping kidneys free from uropathogenic Escherichia coli (UPEC), but we have shown that 2 strains (J96 and 536) can subvert this role and reduce ureteric contractility. To determine whether this is (1) a widespread feature of UPEC, (2) exhibited only by UPEC, and (3) dependent upon type 1 fimbriae, we analyzed strains representing epidemiologically important multilocus sequence types ST131, ST73, and ST95 and non-UPEC E. coli.

Methods: Contractility and calcium transients in intact rat ureters were compared between strains. Mannose and fim mutants were used to investigate the role of type 1 fimbriae.

Results: Non-UPEC had no significant effect on contractility, with a mean decrease after 8 hours of 8.8%, compared with 8.8% in controls. UPEC effects on contractility were strain specific, with decreases from 9.47% to 96.7%. Mannose inhibited the effects of the most potent strains (CFT073 and UTI89) but had variable effects among other UPEC strains. Mutation and complementation studies showed that the effects of the UTI89 cystitis isolate were fimH dependent.

Conclusions: We find that (1) non-UPEC do not affect ureteric contractility, (2) impairment of contractility is a common feature of UPEC, and (3) the mechanism varies between strains, but for the most potent UPEC type 1 fimbriae are involved.

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Related in: MedlinePlus

Effect of type 1 fimbrial mutants on phasic activity in rat ureters. The effects of UTI89 Δfim, UTI89 ΔfimH and UTI89 ΔfimH/pfimH on phasic activity in rat ureters over time are shown. Statistical significance was determined by an unpaired t test (P < .0005) is denoted by an asterisk.
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JIS554F4: Effect of type 1 fimbrial mutants on phasic activity in rat ureters. The effects of UTI89 Δfim, UTI89 ΔfimH and UTI89 ΔfimH/pfimH on phasic activity in rat ureters over time are shown. Statistical significance was determined by an unpaired t test (P < .0005) is denoted by an asterisk.

Mentions: Deletion of the UTI89 (ST95) fim operon eradicates its ability to impair ureteric contractility. Rat ureters exposed to the full fim operon deletion mutant (UTI89 Δfim) showed only small, statistically insignificant decreases in contractile amplitude after 8 hours of exposure (9.1% ± 0.2%) (Figure 4), when compared to the wild-type parental UTI89 (ST95) strain, which induced a 96.8% reduction in contractile amplitude (Table 1). In addition, no significant change in contractile amplitude was observed in ureters exposed lumenally to UTI89 (ST95) lacking fimH (10.2% ± 0.8%), suggesting that contractile impairment is mediated in part by FimH-mediated binding of bacteria with host urothelium. The inhibitory capacity was partially restored (43.9% ± 5.2%) when UTI89 ΔfimH was complemented with wild-type fimH (UTI89 ΔfimH/pfimH) (Figure 4).Figure 4.


Escherichia coli-mediated impairment of ureteric contractility is uropathogenic E. coli specific.

Floyd RV, Upton M, Hultgren SJ, Wray S, Burdyga TV, Winstanley C - J. Infect. Dis. (2012)

Effect of type 1 fimbrial mutants on phasic activity in rat ureters. The effects of UTI89 Δfim, UTI89 ΔfimH and UTI89 ΔfimH/pfimH on phasic activity in rat ureters over time are shown. Statistical significance was determined by an unpaired t test (P < .0005) is denoted by an asterisk.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475635&req=5

JIS554F4: Effect of type 1 fimbrial mutants on phasic activity in rat ureters. The effects of UTI89 Δfim, UTI89 ΔfimH and UTI89 ΔfimH/pfimH on phasic activity in rat ureters over time are shown. Statistical significance was determined by an unpaired t test (P < .0005) is denoted by an asterisk.
Mentions: Deletion of the UTI89 (ST95) fim operon eradicates its ability to impair ureteric contractility. Rat ureters exposed to the full fim operon deletion mutant (UTI89 Δfim) showed only small, statistically insignificant decreases in contractile amplitude after 8 hours of exposure (9.1% ± 0.2%) (Figure 4), when compared to the wild-type parental UTI89 (ST95) strain, which induced a 96.8% reduction in contractile amplitude (Table 1). In addition, no significant change in contractile amplitude was observed in ureters exposed lumenally to UTI89 (ST95) lacking fimH (10.2% ± 0.8%), suggesting that contractile impairment is mediated in part by FimH-mediated binding of bacteria with host urothelium. The inhibitory capacity was partially restored (43.9% ± 5.2%) when UTI89 ΔfimH was complemented with wild-type fimH (UTI89 ΔfimH/pfimH) (Figure 4).Figure 4.

Bottom Line: Non-UPEC had no significant effect on contractility, with a mean decrease after 8 hours of 8.8%, compared with 8.8% in controls.UPEC effects on contractility were strain specific, with decreases from 9.47% to 96.7%.We find that (1) non-UPEC do not affect ureteric contractility, (2) impairment of contractility is a common feature of UPEC, and (3) the mechanism varies between strains, but for the most potent UPEC type 1 fimbriae are involved.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, United Kingdom. floyd78@liv.ac.uk

ABSTRACT

Background: Ureters are fundamental for keeping kidneys free from uropathogenic Escherichia coli (UPEC), but we have shown that 2 strains (J96 and 536) can subvert this role and reduce ureteric contractility. To determine whether this is (1) a widespread feature of UPEC, (2) exhibited only by UPEC, and (3) dependent upon type 1 fimbriae, we analyzed strains representing epidemiologically important multilocus sequence types ST131, ST73, and ST95 and non-UPEC E. coli.

Methods: Contractility and calcium transients in intact rat ureters were compared between strains. Mannose and fim mutants were used to investigate the role of type 1 fimbriae.

Results: Non-UPEC had no significant effect on contractility, with a mean decrease after 8 hours of 8.8%, compared with 8.8% in controls. UPEC effects on contractility were strain specific, with decreases from 9.47% to 96.7%. Mannose inhibited the effects of the most potent strains (CFT073 and UTI89) but had variable effects among other UPEC strains. Mutation and complementation studies showed that the effects of the UTI89 cystitis isolate were fimH dependent.

Conclusions: We find that (1) non-UPEC do not affect ureteric contractility, (2) impairment of contractility is a common feature of UPEC, and (3) the mechanism varies between strains, but for the most potent UPEC type 1 fimbriae are involved.

Show MeSH
Related in: MedlinePlus