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Escherichia coli-mediated impairment of ureteric contractility is uropathogenic E. coli specific.

Floyd RV, Upton M, Hultgren SJ, Wray S, Burdyga TV, Winstanley C - J. Infect. Dis. (2012)

Bottom Line: Non-UPEC had no significant effect on contractility, with a mean decrease after 8 hours of 8.8%, compared with 8.8% in controls.UPEC effects on contractility were strain specific, with decreases from 9.47% to 96.7%.We find that (1) non-UPEC do not affect ureteric contractility, (2) impairment of contractility is a common feature of UPEC, and (3) the mechanism varies between strains, but for the most potent UPEC type 1 fimbriae are involved.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, United Kingdom. floyd78@liv.ac.uk

ABSTRACT

Background: Ureters are fundamental for keeping kidneys free from uropathogenic Escherichia coli (UPEC), but we have shown that 2 strains (J96 and 536) can subvert this role and reduce ureteric contractility. To determine whether this is (1) a widespread feature of UPEC, (2) exhibited only by UPEC, and (3) dependent upon type 1 fimbriae, we analyzed strains representing epidemiologically important multilocus sequence types ST131, ST73, and ST95 and non-UPEC E. coli.

Methods: Contractility and calcium transients in intact rat ureters were compared between strains. Mannose and fim mutants were used to investigate the role of type 1 fimbriae.

Results: Non-UPEC had no significant effect on contractility, with a mean decrease after 8 hours of 8.8%, compared with 8.8% in controls. UPEC effects on contractility were strain specific, with decreases from 9.47% to 96.7%. Mannose inhibited the effects of the most potent strains (CFT073 and UTI89) but had variable effects among other UPEC strains. Mutation and complementation studies showed that the effects of the UTI89 cystitis isolate were fimH dependent.

Conclusions: We find that (1) non-UPEC do not affect ureteric contractility, (2) impairment of contractility is a common feature of UPEC, and (3) the mechanism varies between strains, but for the most potent UPEC type 1 fimbriae are involved.

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Effects of methyl α-d-mannopyranoside (MαDM) on inhibition of phasic ureteric activity mediated by uropathogenic Escherichia coli (UPEC). A, Contractile amplitude in rat ureters exposed lumenally to UPEC M160 in the presence and absence of 25 mM MαDM is shown. B, Example recordings of force and calcium transients from rat ureters loaded with calcium-sensitive Indo 1 and exposed to M160 in the presence and absence of 25 mM MαDM are shown. Statistical significance was determined by an unpaired t test (P < .0005) is denoted by an asterisk.
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JIS554F3: Effects of methyl α-d-mannopyranoside (MαDM) on inhibition of phasic ureteric activity mediated by uropathogenic Escherichia coli (UPEC). A, Contractile amplitude in rat ureters exposed lumenally to UPEC M160 in the presence and absence of 25 mM MαDM is shown. B, Example recordings of force and calcium transients from rat ureters loaded with calcium-sensitive Indo 1 and exposed to M160 in the presence and absence of 25 mM MαDM are shown. Statistical significance was determined by an unpaired t test (P < .0005) is denoted by an asterisk.

Mentions: A dose of 25mM MαDM was able to block the inhibitory effects of M160 (ST131; 11.7% ± 1.3%) (Figure 3) but not EC958 (ST131; 82.1% ± 1.6%) (Table 1). In ureters exposed to M9 (ST73), MαDM was unable to completely block the inhibitory effects of M9 on contractile amplitude but the onset of ureteric impairment was delayed by 2 hours (42.2% ± 7.3 for –MαDM, compared with 38.3% ± 3.5 for +MαDM). The inhibitory effects of UPEC strains UTI89 (ST95) and CFT073 (ST73), which caused the largest impairment of contractility, were abolished in the presence of 25 mM MαDM (10.2% ± 1.7% and 9.5% ± 2.3%, respectively).Figure 3.


Escherichia coli-mediated impairment of ureteric contractility is uropathogenic E. coli specific.

Floyd RV, Upton M, Hultgren SJ, Wray S, Burdyga TV, Winstanley C - J. Infect. Dis. (2012)

Effects of methyl α-d-mannopyranoside (MαDM) on inhibition of phasic ureteric activity mediated by uropathogenic Escherichia coli (UPEC). A, Contractile amplitude in rat ureters exposed lumenally to UPEC M160 in the presence and absence of 25 mM MαDM is shown. B, Example recordings of force and calcium transients from rat ureters loaded with calcium-sensitive Indo 1 and exposed to M160 in the presence and absence of 25 mM MαDM are shown. Statistical significance was determined by an unpaired t test (P < .0005) is denoted by an asterisk.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475635&req=5

JIS554F3: Effects of methyl α-d-mannopyranoside (MαDM) on inhibition of phasic ureteric activity mediated by uropathogenic Escherichia coli (UPEC). A, Contractile amplitude in rat ureters exposed lumenally to UPEC M160 in the presence and absence of 25 mM MαDM is shown. B, Example recordings of force and calcium transients from rat ureters loaded with calcium-sensitive Indo 1 and exposed to M160 in the presence and absence of 25 mM MαDM are shown. Statistical significance was determined by an unpaired t test (P < .0005) is denoted by an asterisk.
Mentions: A dose of 25mM MαDM was able to block the inhibitory effects of M160 (ST131; 11.7% ± 1.3%) (Figure 3) but not EC958 (ST131; 82.1% ± 1.6%) (Table 1). In ureters exposed to M9 (ST73), MαDM was unable to completely block the inhibitory effects of M9 on contractile amplitude but the onset of ureteric impairment was delayed by 2 hours (42.2% ± 7.3 for –MαDM, compared with 38.3% ± 3.5 for +MαDM). The inhibitory effects of UPEC strains UTI89 (ST95) and CFT073 (ST73), which caused the largest impairment of contractility, were abolished in the presence of 25 mM MαDM (10.2% ± 1.7% and 9.5% ± 2.3%, respectively).Figure 3.

Bottom Line: Non-UPEC had no significant effect on contractility, with a mean decrease after 8 hours of 8.8%, compared with 8.8% in controls.UPEC effects on contractility were strain specific, with decreases from 9.47% to 96.7%.We find that (1) non-UPEC do not affect ureteric contractility, (2) impairment of contractility is a common feature of UPEC, and (3) the mechanism varies between strains, but for the most potent UPEC type 1 fimbriae are involved.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, United Kingdom. floyd78@liv.ac.uk

ABSTRACT

Background: Ureters are fundamental for keeping kidneys free from uropathogenic Escherichia coli (UPEC), but we have shown that 2 strains (J96 and 536) can subvert this role and reduce ureteric contractility. To determine whether this is (1) a widespread feature of UPEC, (2) exhibited only by UPEC, and (3) dependent upon type 1 fimbriae, we analyzed strains representing epidemiologically important multilocus sequence types ST131, ST73, and ST95 and non-UPEC E. coli.

Methods: Contractility and calcium transients in intact rat ureters were compared between strains. Mannose and fim mutants were used to investigate the role of type 1 fimbriae.

Results: Non-UPEC had no significant effect on contractility, with a mean decrease after 8 hours of 8.8%, compared with 8.8% in controls. UPEC effects on contractility were strain specific, with decreases from 9.47% to 96.7%. Mannose inhibited the effects of the most potent strains (CFT073 and UTI89) but had variable effects among other UPEC strains. Mutation and complementation studies showed that the effects of the UTI89 cystitis isolate were fimH dependent.

Conclusions: We find that (1) non-UPEC do not affect ureteric contractility, (2) impairment of contractility is a common feature of UPEC, and (3) the mechanism varies between strains, but for the most potent UPEC type 1 fimbriae are involved.

Show MeSH
Related in: MedlinePlus