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Escherichia coli-mediated impairment of ureteric contractility is uropathogenic E. coli specific.

Floyd RV, Upton M, Hultgren SJ, Wray S, Burdyga TV, Winstanley C - J. Infect. Dis. (2012)

Bottom Line: Non-UPEC had no significant effect on contractility, with a mean decrease after 8 hours of 8.8%, compared with 8.8% in controls.UPEC effects on contractility were strain specific, with decreases from 9.47% to 96.7%.We find that (1) non-UPEC do not affect ureteric contractility, (2) impairment of contractility is a common feature of UPEC, and (3) the mechanism varies between strains, but for the most potent UPEC type 1 fimbriae are involved.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, United Kingdom. floyd78@liv.ac.uk

ABSTRACT

Background: Ureters are fundamental for keeping kidneys free from uropathogenic Escherichia coli (UPEC), but we have shown that 2 strains (J96 and 536) can subvert this role and reduce ureteric contractility. To determine whether this is (1) a widespread feature of UPEC, (2) exhibited only by UPEC, and (3) dependent upon type 1 fimbriae, we analyzed strains representing epidemiologically important multilocus sequence types ST131, ST73, and ST95 and non-UPEC E. coli.

Methods: Contractility and calcium transients in intact rat ureters were compared between strains. Mannose and fim mutants were used to investigate the role of type 1 fimbriae.

Results: Non-UPEC had no significant effect on contractility, with a mean decrease after 8 hours of 8.8%, compared with 8.8% in controls. UPEC effects on contractility were strain specific, with decreases from 9.47% to 96.7%. Mannose inhibited the effects of the most potent strains (CFT073 and UTI89) but had variable effects among other UPEC strains. Mutation and complementation studies showed that the effects of the UTI89 cystitis isolate were fimH dependent.

Conclusions: We find that (1) non-UPEC do not affect ureteric contractility, (2) impairment of contractility is a common feature of UPEC, and (3) the mechanism varies between strains, but for the most potent UPEC type 1 fimbriae are involved.

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Effects of Escherichia coli applied to rat ureteric lumen on force and calcium transients. Example recordings of force and calcium transients from rat ureters loaded with calcium-sensitive Indo 1 and exposed to E. coli strains that exhibit low (A, 3770), intermediate (B, M9), and high (C, CFT073) impairment of ureteric contractile amplitude over time. Figures show that impaired ureter activity is mediated by a decrease in the duration and amplitude of the calcium transient.
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JIS554F2: Effects of Escherichia coli applied to rat ureteric lumen on force and calcium transients. Example recordings of force and calcium transients from rat ureters loaded with calcium-sensitive Indo 1 and exposed to E. coli strains that exhibit low (A, 3770), intermediate (B, M9), and high (C, CFT073) impairment of ureteric contractile amplitude over time. Figures show that impaired ureter activity is mediated by a decrease in the duration and amplitude of the calcium transient.

Mentions: Typical traces of phasic activity and calcium transients recorded during exposure to CFT073 (ST73), demonstrated that the loss of activity is mediated by depression of the duration and amplitude of the calcium transient that drives contractility (Figure 2C). This effect is consistent with our previous studies investigating the effect of well-characterized pyelonephritis strains of E. coli J96 and 536 in intact rat ureters [34], which showed similar mean inhibition (88.8% and 86.6% of control, respectively).Figure 2.


Escherichia coli-mediated impairment of ureteric contractility is uropathogenic E. coli specific.

Floyd RV, Upton M, Hultgren SJ, Wray S, Burdyga TV, Winstanley C - J. Infect. Dis. (2012)

Effects of Escherichia coli applied to rat ureteric lumen on force and calcium transients. Example recordings of force and calcium transients from rat ureters loaded with calcium-sensitive Indo 1 and exposed to E. coli strains that exhibit low (A, 3770), intermediate (B, M9), and high (C, CFT073) impairment of ureteric contractile amplitude over time. Figures show that impaired ureter activity is mediated by a decrease in the duration and amplitude of the calcium transient.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475635&req=5

JIS554F2: Effects of Escherichia coli applied to rat ureteric lumen on force and calcium transients. Example recordings of force and calcium transients from rat ureters loaded with calcium-sensitive Indo 1 and exposed to E. coli strains that exhibit low (A, 3770), intermediate (B, M9), and high (C, CFT073) impairment of ureteric contractile amplitude over time. Figures show that impaired ureter activity is mediated by a decrease in the duration and amplitude of the calcium transient.
Mentions: Typical traces of phasic activity and calcium transients recorded during exposure to CFT073 (ST73), demonstrated that the loss of activity is mediated by depression of the duration and amplitude of the calcium transient that drives contractility (Figure 2C). This effect is consistent with our previous studies investigating the effect of well-characterized pyelonephritis strains of E. coli J96 and 536 in intact rat ureters [34], which showed similar mean inhibition (88.8% and 86.6% of control, respectively).Figure 2.

Bottom Line: Non-UPEC had no significant effect on contractility, with a mean decrease after 8 hours of 8.8%, compared with 8.8% in controls.UPEC effects on contractility were strain specific, with decreases from 9.47% to 96.7%.We find that (1) non-UPEC do not affect ureteric contractility, (2) impairment of contractility is a common feature of UPEC, and (3) the mechanism varies between strains, but for the most potent UPEC type 1 fimbriae are involved.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, United Kingdom. floyd78@liv.ac.uk

ABSTRACT

Background: Ureters are fundamental for keeping kidneys free from uropathogenic Escherichia coli (UPEC), but we have shown that 2 strains (J96 and 536) can subvert this role and reduce ureteric contractility. To determine whether this is (1) a widespread feature of UPEC, (2) exhibited only by UPEC, and (3) dependent upon type 1 fimbriae, we analyzed strains representing epidemiologically important multilocus sequence types ST131, ST73, and ST95 and non-UPEC E. coli.

Methods: Contractility and calcium transients in intact rat ureters were compared between strains. Mannose and fim mutants were used to investigate the role of type 1 fimbriae.

Results: Non-UPEC had no significant effect on contractility, with a mean decrease after 8 hours of 8.8%, compared with 8.8% in controls. UPEC effects on contractility were strain specific, with decreases from 9.47% to 96.7%. Mannose inhibited the effects of the most potent strains (CFT073 and UTI89) but had variable effects among other UPEC strains. Mutation and complementation studies showed that the effects of the UTI89 cystitis isolate were fimH dependent.

Conclusions: We find that (1) non-UPEC do not affect ureteric contractility, (2) impairment of contractility is a common feature of UPEC, and (3) the mechanism varies between strains, but for the most potent UPEC type 1 fimbriae are involved.

Show MeSH
Related in: MedlinePlus