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The effect of post-treatment N-acetylcysteine in LPS-induced acute lung injury of rats.

Choi JS, Lee HS, Seo KH, Na JO, Kim YH, Uh ST, Park CS, Oh MH, Lee SH, Kim YT - Tuberc Respir Dis (Seoul) (2012)

Bottom Line: The concentration of LPO in NAC treatment group was significantly lower than that of LPS group (5.5±2.8 nmol/mL vs. 16.5±1.6 nmol/mL) (p=0.001).The activity of MPO in NAC treatment group was significantly lower than that of LPS group (6.4±1.8 unit/g vs. 11.2±6.3 unit/g, tissue) (p<0.048).The concentration of NF-κB in NAC treatment group was significantly lower than that of LPS group (0.3±0.1 ng/µL vs. 0.4±0.2 ng/µL) (p=0.0001).

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Clinical Research Institute, Soonchunhyang University College of Medicine, Cheonan, Korea.

ABSTRACT

Background: Oxidation plays an important role in acute lung injury. This study was conducted in order to elucidate the effect of repetitive post-treatment of N-acetylcysteine (NAC) in lipopolysaccaride (LPS)-induced acute lung injury (ALI) of rats.

Methods: Six-week-old male Sprague-Dawley rats were divided into 4 groups. LPS (Escherichia coli 5 mg/kg) was administered intravenously via the tail vein. NAC (20 mg/kg) was injected intraperitoneally 3, 6, and 12 hours after LPS injection. Broncho-alveolar lavage fluid (BALF) and lung tissues were obtained to evaluate the ALI at 24 hours after LPS injection. The concentration of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) were measured in BALF. Nuclear factor κB (NF-κB), lipid peroxidation (LPO), and myeloperoxidase (MPO) were measured using lung tissues. Micro-computed tomography (micro-CT) images were examined in each group at 72 hours apart from the main experiments in order to observe the delayed effects of NAC.

Results: TNF-α and IL-1β concentration in BALF were not different between LPS and NAC treatment groups. The concentration of LPO in NAC treatment group was significantly lower than that of LPS group (5.5±2.8 nmol/mL vs. 16.5±1.6 nmol/mL) (p=0.001). The activity of MPO in NAC treatment group was significantly lower than that of LPS group (6.4±1.8 unit/g vs. 11.2±6.3 unit/g, tissue) (p<0.048). The concentration of NF-κB in NAC treatment group was significantly lower than that of LPS group (0.3±0.1 ng/µL vs. 0.4±0.2 ng/µL) (p=0.0001). Micro-CT showed less extent of lung injury in NAC treatment than LPS group.

Conclusion: After induction of ALI with lipopolysaccharide, the therapeutic administration of NAC partially attenuated the extent of ALI through the inhibition of NF-κB activation.

No MeSH data available.


Related in: MedlinePlus

The effects of N-acetylcysteine (NAC) of nuclear factor κB (NF-κB) and lipid peroxidation in lipopolysaccharide (LPS) induced acute lung injury. (A) Lipid peroxidation concentrations in lung tissue. In NAC treatment (LPS+NAC) group, lipid peroxidation concentration significantly decreased than that in LPS group. (B) NF-κB concentrations in lung tissue. NF-κB concentration in NAC treatment group significantly decreased more than that in LPS group. Box table: median (25~75%); except out-layer data.
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Figure 1: The effects of N-acetylcysteine (NAC) of nuclear factor κB (NF-κB) and lipid peroxidation in lipopolysaccharide (LPS) induced acute lung injury. (A) Lipid peroxidation concentrations in lung tissue. In NAC treatment (LPS+NAC) group, lipid peroxidation concentration significantly decreased than that in LPS group. (B) NF-κB concentrations in lung tissue. NF-κB concentration in NAC treatment group significantly decreased more than that in LPS group. Box table: median (25~75%); except out-layer data.

Mentions: LPO concentration (nmol/mL) in LPS group was higher compared to SC and NC groups (16.5±1.6 vs. 4.3±3.8, 3.5±2.0) (p=0.0001) but significantly lower when comparing with NACTX group (5.5±2.8) (p=0.001) (Table 3, Figure 1A). The MPO activity in the left lung tissues (unit/g, lung tissue) significantly decreased in NACTX compared to LPS group (6.4±1.8 vs. 11.2±6.3) (p=0.048) (Table 3). LPS group (0.4±0.2) showed the highest NF-κB concentration (ng/µL) compared to SC (0.1±0.0), and NC (0.11±0.0) groups (p=0.0001) and it decreased significantly compared to NACTX group (0.3±0.1) (p=0.006) (Table 3, Figure 1B).


The effect of post-treatment N-acetylcysteine in LPS-induced acute lung injury of rats.

Choi JS, Lee HS, Seo KH, Na JO, Kim YH, Uh ST, Park CS, Oh MH, Lee SH, Kim YT - Tuberc Respir Dis (Seoul) (2012)

The effects of N-acetylcysteine (NAC) of nuclear factor κB (NF-κB) and lipid peroxidation in lipopolysaccharide (LPS) induced acute lung injury. (A) Lipid peroxidation concentrations in lung tissue. In NAC treatment (LPS+NAC) group, lipid peroxidation concentration significantly decreased than that in LPS group. (B) NF-κB concentrations in lung tissue. NF-κB concentration in NAC treatment group significantly decreased more than that in LPS group. Box table: median (25~75%); except out-layer data.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3475471&req=5

Figure 1: The effects of N-acetylcysteine (NAC) of nuclear factor κB (NF-κB) and lipid peroxidation in lipopolysaccharide (LPS) induced acute lung injury. (A) Lipid peroxidation concentrations in lung tissue. In NAC treatment (LPS+NAC) group, lipid peroxidation concentration significantly decreased than that in LPS group. (B) NF-κB concentrations in lung tissue. NF-κB concentration in NAC treatment group significantly decreased more than that in LPS group. Box table: median (25~75%); except out-layer data.
Mentions: LPO concentration (nmol/mL) in LPS group was higher compared to SC and NC groups (16.5±1.6 vs. 4.3±3.8, 3.5±2.0) (p=0.0001) but significantly lower when comparing with NACTX group (5.5±2.8) (p=0.001) (Table 3, Figure 1A). The MPO activity in the left lung tissues (unit/g, lung tissue) significantly decreased in NACTX compared to LPS group (6.4±1.8 vs. 11.2±6.3) (p=0.048) (Table 3). LPS group (0.4±0.2) showed the highest NF-κB concentration (ng/µL) compared to SC (0.1±0.0), and NC (0.11±0.0) groups (p=0.0001) and it decreased significantly compared to NACTX group (0.3±0.1) (p=0.006) (Table 3, Figure 1B).

Bottom Line: The concentration of LPO in NAC treatment group was significantly lower than that of LPS group (5.5±2.8 nmol/mL vs. 16.5±1.6 nmol/mL) (p=0.001).The activity of MPO in NAC treatment group was significantly lower than that of LPS group (6.4±1.8 unit/g vs. 11.2±6.3 unit/g, tissue) (p<0.048).The concentration of NF-κB in NAC treatment group was significantly lower than that of LPS group (0.3±0.1 ng/µL vs. 0.4±0.2 ng/µL) (p=0.0001).

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Clinical Research Institute, Soonchunhyang University College of Medicine, Cheonan, Korea.

ABSTRACT

Background: Oxidation plays an important role in acute lung injury. This study was conducted in order to elucidate the effect of repetitive post-treatment of N-acetylcysteine (NAC) in lipopolysaccaride (LPS)-induced acute lung injury (ALI) of rats.

Methods: Six-week-old male Sprague-Dawley rats were divided into 4 groups. LPS (Escherichia coli 5 mg/kg) was administered intravenously via the tail vein. NAC (20 mg/kg) was injected intraperitoneally 3, 6, and 12 hours after LPS injection. Broncho-alveolar lavage fluid (BALF) and lung tissues were obtained to evaluate the ALI at 24 hours after LPS injection. The concentration of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) were measured in BALF. Nuclear factor κB (NF-κB), lipid peroxidation (LPO), and myeloperoxidase (MPO) were measured using lung tissues. Micro-computed tomography (micro-CT) images were examined in each group at 72 hours apart from the main experiments in order to observe the delayed effects of NAC.

Results: TNF-α and IL-1β concentration in BALF were not different between LPS and NAC treatment groups. The concentration of LPO in NAC treatment group was significantly lower than that of LPS group (5.5±2.8 nmol/mL vs. 16.5±1.6 nmol/mL) (p=0.001). The activity of MPO in NAC treatment group was significantly lower than that of LPS group (6.4±1.8 unit/g vs. 11.2±6.3 unit/g, tissue) (p<0.048). The concentration of NF-κB in NAC treatment group was significantly lower than that of LPS group (0.3±0.1 ng/µL vs. 0.4±0.2 ng/µL) (p=0.0001). Micro-CT showed less extent of lung injury in NAC treatment than LPS group.

Conclusion: After induction of ALI with lipopolysaccharide, the therapeutic administration of NAC partially attenuated the extent of ALI through the inhibition of NF-κB activation.

No MeSH data available.


Related in: MedlinePlus