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hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

Wu M, Sun Q, Guo X, Liu H - Cell Biol Int Rep (2010) (2012)

Bottom Line: Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case.Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells.The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology and Research Center of Developmental Biology, Second Military Medical University, Shanghai, 200433, People's Republic of China.

ABSTRACT
DP (dermal papilla) is a mesenchyme-derived structure situated at the base of the HF (hair follicle) that plays an important role in embryonic hair morphogenesis and maintenance of the hair growth cycle. hMSCs (human mesenchymal stem cells) have gained widespread attention in the field of tissue engineering, but not much is known about the differentiation of hMSCs into DP cells. hMSCs involved in HF formation were examined in our previous study. Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case. During the differentiation process, the expression of versican, CD133, SCF (stem cell factor), ET-1 (endothelin-1) and bFGF (basic fibroblast growth factor) increased. Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells. The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF. The data suggest that hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

No MeSH data available.


Related in: MedlinePlus

HLA-I and keratin expression during HF formation (7 daysafter intracutaneous injection)Green, keratin; red: HLA-I. A: Scalebar = 30 μm. B: scalebar = 50 μm.
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Figure 4: HLA-I and keratin expression during HF formation (7 daysafter intracutaneous injection)Green, keratin; red: HLA-I. A: Scalebar = 30 μm. B: scalebar = 50 μm.

Mentions: hMSCs were confirmed in HF formation by HLA-I detection (Figures 4A and 4B). In some HF,HLA-I was expressed in the sites of HF (Figure 4B), but for other HF only the DP was intenselystained in the lower portion of HFs (Figure 4A).


hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

Wu M, Sun Q, Guo X, Liu H - Cell Biol Int Rep (2010) (2012)

HLA-I and keratin expression during HF formation (7 daysafter intracutaneous injection)Green, keratin; red: HLA-I. A: Scalebar = 30 μm. B: scalebar = 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475446&req=5

Figure 4: HLA-I and keratin expression during HF formation (7 daysafter intracutaneous injection)Green, keratin; red: HLA-I. A: Scalebar = 30 μm. B: scalebar = 50 μm.
Mentions: hMSCs were confirmed in HF formation by HLA-I detection (Figures 4A and 4B). In some HF,HLA-I was expressed in the sites of HF (Figure 4B), but for other HF only the DP was intenselystained in the lower portion of HFs (Figure 4A).

Bottom Line: Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case.Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells.The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology and Research Center of Developmental Biology, Second Military Medical University, Shanghai, 200433, People's Republic of China.

ABSTRACT
DP (dermal papilla) is a mesenchyme-derived structure situated at the base of the HF (hair follicle) that plays an important role in embryonic hair morphogenesis and maintenance of the hair growth cycle. hMSCs (human mesenchymal stem cells) have gained widespread attention in the field of tissue engineering, but not much is known about the differentiation of hMSCs into DP cells. hMSCs involved in HF formation were examined in our previous study. Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case. During the differentiation process, the expression of versican, CD133, SCF (stem cell factor), ET-1 (endothelin-1) and bFGF (basic fibroblast growth factor) increased. Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells. The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF. The data suggest that hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

No MeSH data available.


Related in: MedlinePlus