Limits...
hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

Wu M, Sun Q, Guo X, Liu H - Cell Biol Int Rep (2010) (2012)

Bottom Line: Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case.Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells.The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology and Research Center of Developmental Biology, Second Military Medical University, Shanghai, 200433, People's Republic of China.

ABSTRACT
DP (dermal papilla) is a mesenchyme-derived structure situated at the base of the HF (hair follicle) that plays an important role in embryonic hair morphogenesis and maintenance of the hair growth cycle. hMSCs (human mesenchymal stem cells) have gained widespread attention in the field of tissue engineering, but not much is known about the differentiation of hMSCs into DP cells. hMSCs involved in HF formation were examined in our previous study. Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case. During the differentiation process, the expression of versican, CD133, SCF (stem cell factor), ET-1 (endothelin-1) and bFGF (basic fibroblast growth factor) increased. Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells. The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF. The data suggest that hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

No MeSH data available.


Related in: MedlinePlus

H&E analysis of morphological difference(A) 1 day after injection and (B)30 days after injection show the subcutaneous tissues ofhMSCs intracutaneous injection group mice.(C) Shows subcutaneous tissues of 7 daysafter keratinocyte intracutaneous injection.(D, E) 7 days afterfibroblast and DMEM injection, respectively.(F) Shows the subcutaneous tissues ofnormal BALB/c-nu/nu mice. (A) ×100;(D, F) ×200;(B, C and E)×400.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3475446&req=5

Figure 3: H&E analysis of morphological difference(A) 1 day after injection and (B)30 days after injection show the subcutaneous tissues ofhMSCs intracutaneous injection group mice.(C) Shows subcutaneous tissues of 7 daysafter keratinocyte intracutaneous injection.(D, E) 7 days afterfibroblast and DMEM injection, respectively.(F) Shows the subcutaneous tissues ofnormal BALB/c-nu/nu mice. (A) ×100;(D, F) ×200;(B, C and E)×400.

Mentions: H&E sections of the subcutaneous tissue structure of the hMSCsinjection group and control mice showed many differences (Figure 3). HF appeared in thehMSCs injection group (Figures 3A and3B). For the keratinocyte injection group, a hard cellmass formed at the injection site (Figure 3C). Compared with the hMSCs injection group, noHF formed in fibroblast or DMEM injection groups (Figures 3D and 3E).


hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

Wu M, Sun Q, Guo X, Liu H - Cell Biol Int Rep (2010) (2012)

H&E analysis of morphological difference(A) 1 day after injection and (B)30 days after injection show the subcutaneous tissues ofhMSCs intracutaneous injection group mice.(C) Shows subcutaneous tissues of 7 daysafter keratinocyte intracutaneous injection.(D, E) 7 days afterfibroblast and DMEM injection, respectively.(F) Shows the subcutaneous tissues ofnormal BALB/c-nu/nu mice. (A) ×100;(D, F) ×200;(B, C and E)×400.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475446&req=5

Figure 3: H&E analysis of morphological difference(A) 1 day after injection and (B)30 days after injection show the subcutaneous tissues ofhMSCs intracutaneous injection group mice.(C) Shows subcutaneous tissues of 7 daysafter keratinocyte intracutaneous injection.(D, E) 7 days afterfibroblast and DMEM injection, respectively.(F) Shows the subcutaneous tissues ofnormal BALB/c-nu/nu mice. (A) ×100;(D, F) ×200;(B, C and E)×400.
Mentions: H&E sections of the subcutaneous tissue structure of the hMSCsinjection group and control mice showed many differences (Figure 3). HF appeared in thehMSCs injection group (Figures 3A and3B). For the keratinocyte injection group, a hard cellmass formed at the injection site (Figure 3C). Compared with the hMSCs injection group, noHF formed in fibroblast or DMEM injection groups (Figures 3D and 3E).

Bottom Line: Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case.Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells.The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology and Research Center of Developmental Biology, Second Military Medical University, Shanghai, 200433, People's Republic of China.

ABSTRACT
DP (dermal papilla) is a mesenchyme-derived structure situated at the base of the HF (hair follicle) that plays an important role in embryonic hair morphogenesis and maintenance of the hair growth cycle. hMSCs (human mesenchymal stem cells) have gained widespread attention in the field of tissue engineering, but not much is known about the differentiation of hMSCs into DP cells. hMSCs involved in HF formation were examined in our previous study. Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case. During the differentiation process, the expression of versican, CD133, SCF (stem cell factor), ET-1 (endothelin-1) and bFGF (basic fibroblast growth factor) increased. Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells. The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF. The data suggest that hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

No MeSH data available.


Related in: MedlinePlus