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hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

Wu M, Sun Q, Guo X, Liu H - Cell Biol Int Rep (2010) (2012)

Bottom Line: Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case.Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells.The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology and Research Center of Developmental Biology, Second Military Medical University, Shanghai, 200433, People's Republic of China.

ABSTRACT
DP (dermal papilla) is a mesenchyme-derived structure situated at the base of the HF (hair follicle) that plays an important role in embryonic hair morphogenesis and maintenance of the hair growth cycle. hMSCs (human mesenchymal stem cells) have gained widespread attention in the field of tissue engineering, but not much is known about the differentiation of hMSCs into DP cells. hMSCs involved in HF formation were examined in our previous study. Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case. During the differentiation process, the expression of versican, CD133, SCF (stem cell factor), ET-1 (endothelin-1) and bFGF (basic fibroblast growth factor) increased. Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells. The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF. The data suggest that hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

No MeSH data available.


Related in: MedlinePlus

hMSCs co-cultured with DP cells(A–C) The co-culture system.(A) Experimental group.(B, C) Control groups. A1: hMSCscultured alone. A2: hMSCs co-cultured with DP cells for 7days. Cell morphology changed from fibroblastic toaggregative behaviour. A3: detection of versican in hMSCsco-cultured with DP cells, cytoplasm positive.(D) Detection of SCF, ET-1 and bFGFexpression in hMSCs co-cultured with DP cells.Quantification of secretion bFGF, ET-1 and SCF where100% corresponds to the amount of bFGF, ET-1 andSCF secreted after hMSCs were co-cultured with DP cellsfor 7 days. (E) The aggregate number of percm2 of hMSCs and hMSCs co-cultured withDP cells for 7 days. Flow cytometry was used to determinethe proportion of CD133-positive cells from hMSCsco-cultured with DP cells. (F) Negativecontrol. (G) CD133 detection, positives were47%. A1, A2: ×200; A3: ×400.*P<0.05
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Figure 2: hMSCs co-cultured with DP cells(A–C) The co-culture system.(A) Experimental group.(B, C) Control groups. A1: hMSCscultured alone. A2: hMSCs co-cultured with DP cells for 7days. Cell morphology changed from fibroblastic toaggregative behaviour. A3: detection of versican in hMSCsco-cultured with DP cells, cytoplasm positive.(D) Detection of SCF, ET-1 and bFGFexpression in hMSCs co-cultured with DP cells.Quantification of secretion bFGF, ET-1 and SCF where100% corresponds to the amount of bFGF, ET-1 andSCF secreted after hMSCs were co-cultured with DP cellsfor 7 days. (E) The aggregate number of percm2 of hMSCs and hMSCs co-cultured withDP cells for 7 days. Flow cytometry was used to determinethe proportion of CD133-positive cells from hMSCsco-cultured with DP cells. (F) Negativecontrol. (G) CD133 detection, positives were47%. A1, A2: ×200; A3: ×400.*P<0.05

Mentions: A cell co-culture system was used to investigate the intercellularinteraction and cells differentiation potential. Photomicrographs ofnormal hMSCs show fibroblast morphology. Confluent hMSCs formwhirlpool-like fibroblasts (Figure2A1). After 7 days of co-culturing in indirect contactwith DP cells, the hMSCs showed aggregation (Figure 2A2). The aggregate numbers percm2 in hMSCs and hMSCs co-cultured with DP cells werecounted, and the data are shown in Figure 2(E), with hMSCs co-cultured with DP cells beinggreater than hMSCs cultured alone.


hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

Wu M, Sun Q, Guo X, Liu H - Cell Biol Int Rep (2010) (2012)

hMSCs co-cultured with DP cells(A–C) The co-culture system.(A) Experimental group.(B, C) Control groups. A1: hMSCscultured alone. A2: hMSCs co-cultured with DP cells for 7days. Cell morphology changed from fibroblastic toaggregative behaviour. A3: detection of versican in hMSCsco-cultured with DP cells, cytoplasm positive.(D) Detection of SCF, ET-1 and bFGFexpression in hMSCs co-cultured with DP cells.Quantification of secretion bFGF, ET-1 and SCF where100% corresponds to the amount of bFGF, ET-1 andSCF secreted after hMSCs were co-cultured with DP cellsfor 7 days. (E) The aggregate number of percm2 of hMSCs and hMSCs co-cultured withDP cells for 7 days. Flow cytometry was used to determinethe proportion of CD133-positive cells from hMSCsco-cultured with DP cells. (F) Negativecontrol. (G) CD133 detection, positives were47%. A1, A2: ×200; A3: ×400.*P<0.05
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475446&req=5

Figure 2: hMSCs co-cultured with DP cells(A–C) The co-culture system.(A) Experimental group.(B, C) Control groups. A1: hMSCscultured alone. A2: hMSCs co-cultured with DP cells for 7days. Cell morphology changed from fibroblastic toaggregative behaviour. A3: detection of versican in hMSCsco-cultured with DP cells, cytoplasm positive.(D) Detection of SCF, ET-1 and bFGFexpression in hMSCs co-cultured with DP cells.Quantification of secretion bFGF, ET-1 and SCF where100% corresponds to the amount of bFGF, ET-1 andSCF secreted after hMSCs were co-cultured with DP cellsfor 7 days. (E) The aggregate number of percm2 of hMSCs and hMSCs co-cultured withDP cells for 7 days. Flow cytometry was used to determinethe proportion of CD133-positive cells from hMSCsco-cultured with DP cells. (F) Negativecontrol. (G) CD133 detection, positives were47%. A1, A2: ×200; A3: ×400.*P<0.05
Mentions: A cell co-culture system was used to investigate the intercellularinteraction and cells differentiation potential. Photomicrographs ofnormal hMSCs show fibroblast morphology. Confluent hMSCs formwhirlpool-like fibroblasts (Figure2A1). After 7 days of co-culturing in indirect contactwith DP cells, the hMSCs showed aggregation (Figure 2A2). The aggregate numbers percm2 in hMSCs and hMSCs co-cultured with DP cells werecounted, and the data are shown in Figure 2(E), with hMSCs co-cultured with DP cells beinggreater than hMSCs cultured alone.

Bottom Line: Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case.Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells.The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology and Research Center of Developmental Biology, Second Military Medical University, Shanghai, 200433, People's Republic of China.

ABSTRACT
DP (dermal papilla) is a mesenchyme-derived structure situated at the base of the HF (hair follicle) that plays an important role in embryonic hair morphogenesis and maintenance of the hair growth cycle. hMSCs (human mesenchymal stem cells) have gained widespread attention in the field of tissue engineering, but not much is known about the differentiation of hMSCs into DP cells. hMSCs involved in HF formation were examined in our previous study. Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case. During the differentiation process, the expression of versican, CD133, SCF (stem cell factor), ET-1 (endothelin-1) and bFGF (basic fibroblast growth factor) increased. Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells. The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF. The data suggest that hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

No MeSH data available.


Related in: MedlinePlus