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hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

Wu M, Sun Q, Guo X, Liu H - Cell Biol Int Rep (2010) (2012)

Bottom Line: Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case.Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells.The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology and Research Center of Developmental Biology, Second Military Medical University, Shanghai, 200433, People's Republic of China.

ABSTRACT
DP (dermal papilla) is a mesenchyme-derived structure situated at the base of the HF (hair follicle) that plays an important role in embryonic hair morphogenesis and maintenance of the hair growth cycle. hMSCs (human mesenchymal stem cells) have gained widespread attention in the field of tissue engineering, but not much is known about the differentiation of hMSCs into DP cells. hMSCs involved in HF formation were examined in our previous study. Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case. During the differentiation process, the expression of versican, CD133, SCF (stem cell factor), ET-1 (endothelin-1) and bFGF (basic fibroblast growth factor) increased. Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells. The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF. The data suggest that hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

No MeSH data available.


Related in: MedlinePlus

DP cells isolation and identification(A) cells outgrown from DP after 2 days culture.(B) Passage 8 DP cell culture, wherethe aggregation behaviour has disappeared.(C, D) DP cells positive forα-SMA, indicating mesoblast origin. (E)Versican distribution in DP cells. (F,G) Flow cytometry was used to determinethe proportion of CD133-positive cells from passage 3 DPcells. (F) Negative control. (G)CD133 detection, the positives were 78%.(H) RT–PCR analysis of passage 3DP cells. From left to right: 1 lane: marker; 2 lane: SCF;3 lane: ET-1; 4 lane: bFGF; 5 lane: no RT control.A, B, C:×100; D: ×200; E: ×400.
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Figure 1: DP cells isolation and identification(A) cells outgrown from DP after 2 days culture.(B) Passage 8 DP cell culture, wherethe aggregation behaviour has disappeared.(C, D) DP cells positive forα-SMA, indicating mesoblast origin. (E)Versican distribution in DP cells. (F,G) Flow cytometry was used to determinethe proportion of CD133-positive cells from passage 3 DPcells. (F) Negative control. (G)CD133 detection, the positives were 78%.(H) RT–PCR analysis of passage 3DP cells. From left to right: 1 lane: marker; 2 lane: SCF;3 lane: ET-1; 4 lane: bFGF; 5 lane: no RT control.A, B, C:×100; D: ×200; E: ×400.

Mentions: DP cells were initially grown on plastic. DP cells grow out afterculturing for 48 h (Figure 1A).Three days later, 40–50% of attached cells showedfusion. The cells continued to move away from the DP until confluencewas attained, after which they were sub-cultured. DP cells outgrowthswere sub-cultured using 0.25% trypsin in PBS/EDTA (0.2 mg/ml).No difference in behaviour was observed between DP cells on plastic,but a prerequisite for cell outgrowth was that the DP had attached.Cells were cultured beyond passage 8 for 6 human DP cell strains. Theaggregation behaviour was maintained over 6 passages, and then slowlydisappeared as the cultural time prolonged (Figure 1B).


hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

Wu M, Sun Q, Guo X, Liu H - Cell Biol Int Rep (2010) (2012)

DP cells isolation and identification(A) cells outgrown from DP after 2 days culture.(B) Passage 8 DP cell culture, wherethe aggregation behaviour has disappeared.(C, D) DP cells positive forα-SMA, indicating mesoblast origin. (E)Versican distribution in DP cells. (F,G) Flow cytometry was used to determinethe proportion of CD133-positive cells from passage 3 DPcells. (F) Negative control. (G)CD133 detection, the positives were 78%.(H) RT–PCR analysis of passage 3DP cells. From left to right: 1 lane: marker; 2 lane: SCF;3 lane: ET-1; 4 lane: bFGF; 5 lane: no RT control.A, B, C:×100; D: ×200; E: ×400.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475446&req=5

Figure 1: DP cells isolation and identification(A) cells outgrown from DP after 2 days culture.(B) Passage 8 DP cell culture, wherethe aggregation behaviour has disappeared.(C, D) DP cells positive forα-SMA, indicating mesoblast origin. (E)Versican distribution in DP cells. (F,G) Flow cytometry was used to determinethe proportion of CD133-positive cells from passage 3 DPcells. (F) Negative control. (G)CD133 detection, the positives were 78%.(H) RT–PCR analysis of passage 3DP cells. From left to right: 1 lane: marker; 2 lane: SCF;3 lane: ET-1; 4 lane: bFGF; 5 lane: no RT control.A, B, C:×100; D: ×200; E: ×400.
Mentions: DP cells were initially grown on plastic. DP cells grow out afterculturing for 48 h (Figure 1A).Three days later, 40–50% of attached cells showedfusion. The cells continued to move away from the DP until confluencewas attained, after which they were sub-cultured. DP cells outgrowthswere sub-cultured using 0.25% trypsin in PBS/EDTA (0.2 mg/ml).No difference in behaviour was observed between DP cells on plastic,but a prerequisite for cell outgrowth was that the DP had attached.Cells were cultured beyond passage 8 for 6 human DP cell strains. Theaggregation behaviour was maintained over 6 passages, and then slowlydisappeared as the cultural time prolonged (Figure 1B).

Bottom Line: Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case.Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells.The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology and Research Center of Developmental Biology, Second Military Medical University, Shanghai, 200433, People's Republic of China.

ABSTRACT
DP (dermal papilla) is a mesenchyme-derived structure situated at the base of the HF (hair follicle) that plays an important role in embryonic hair morphogenesis and maintenance of the hair growth cycle. hMSCs (human mesenchymal stem cells) have gained widespread attention in the field of tissue engineering, but not much is known about the differentiation of hMSCs into DP cells. hMSCs involved in HF formation were examined in our previous study. Here, we have explored the differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP cells, which proved to be the case. During the differentiation process, the expression of versican, CD133, SCF (stem cell factor), ET-1 (endothelin-1) and bFGF (basic fibroblast growth factor) increased. Compared with hMSCs alone, the aggregate number clearly increased when co-cultured with DP cells. The expression in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the newly formed HF. The data suggest that hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.

No MeSH data available.


Related in: MedlinePlus