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Human endometrial stem cells as a new source for programming to neural cells.

Mobarakeh ZT, Ai J, Yazdani F, Sorkhabadi SM, Ghanbari Z, Javidan AN, Mortazavi-Tabatabaei SA, Massumi M, Barough SE - Cell Biol Int Rep (2010) (2012)

Bottom Line: The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT-PCR (reverse transcription-PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs.The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry.In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT
Human EnSC (endometrial-derived stem cell) is an abundant and easily available source for cell replacement therapy. Many investigations have shown the potency of the cells to differentiate into several mesoderm-derived cell lineages, including osteocytes and adipocytes. Here, the potency of EnSC in neural differentiation has been investigated. Flow cytometric analysis showed that they were positive for CD90, CD105, OCT4, CD44 and negative for CD31, CD34, CD133. The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT-PCR (reverse transcription-PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs. The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry. In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

No MeSH data available.


Related in: MedlinePlus

Neuro-glial-related gene expression analysis of EnSC 7 days PT (A) and 12 days PT (B) treatment and control (C) for the using RT–PCRβ-Actin was used as internal standard.
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Figure 6: Neuro-glial-related gene expression analysis of EnSC 7 days PT (A) and 12 days PT (B) treatment and control (C) for the using RT–PCRβ-Actin was used as internal standard.

Mentions: To investigate the expression of neural markers at the level of mRNA, RT–PCR was carried out (Figure 6). The expression of MAP2, which has already been shown at the level of protein (Figure 5A′), was also confirmed at the mRNA level at both 7 and 12 days PT. However, the expression of GABA (γ-aminobutyric acid) and Nestin transcripts, which were not detected in EnSC cells (Figure 6C), was detected after differentiation in EnSC-derived neuronal-like cells at both 7 and 12 days PT (Figures 6A and 6B), implying that they were promoting neuronal programming in differentiated cells. In a control group, expression of vimentin as in the treatment group was detected. To investigate the derivation of astrocyte and oligodendrocyte from EnSC upon differentiation using the abovementioned protocol, the expression of GFAP (glial fibrillary acidic protein) and Olig1 as specific markers of astrocytes and oligodendrocyte respectively was analysed by RT–PCR. Expression of these two markers was not detected 7 days PT, while they were clearly expressed at 12 days. The result, at least in the level of mRNA, implies that these cells can adopt a neuronal differentiation pathway in a short time compared with data on other adult stem cells (Bossolasco et al., 2005; Chua et al., 2009), and with more time differentiation into other neural cell lineages will occur.


Human endometrial stem cells as a new source for programming to neural cells.

Mobarakeh ZT, Ai J, Yazdani F, Sorkhabadi SM, Ghanbari Z, Javidan AN, Mortazavi-Tabatabaei SA, Massumi M, Barough SE - Cell Biol Int Rep (2010) (2012)

Neuro-glial-related gene expression analysis of EnSC 7 days PT (A) and 12 days PT (B) treatment and control (C) for the using RT–PCRβ-Actin was used as internal standard.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475442&req=5

Figure 6: Neuro-glial-related gene expression analysis of EnSC 7 days PT (A) and 12 days PT (B) treatment and control (C) for the using RT–PCRβ-Actin was used as internal standard.
Mentions: To investigate the expression of neural markers at the level of mRNA, RT–PCR was carried out (Figure 6). The expression of MAP2, which has already been shown at the level of protein (Figure 5A′), was also confirmed at the mRNA level at both 7 and 12 days PT. However, the expression of GABA (γ-aminobutyric acid) and Nestin transcripts, which were not detected in EnSC cells (Figure 6C), was detected after differentiation in EnSC-derived neuronal-like cells at both 7 and 12 days PT (Figures 6A and 6B), implying that they were promoting neuronal programming in differentiated cells. In a control group, expression of vimentin as in the treatment group was detected. To investigate the derivation of astrocyte and oligodendrocyte from EnSC upon differentiation using the abovementioned protocol, the expression of GFAP (glial fibrillary acidic protein) and Olig1 as specific markers of astrocytes and oligodendrocyte respectively was analysed by RT–PCR. Expression of these two markers was not detected 7 days PT, while they were clearly expressed at 12 days. The result, at least in the level of mRNA, implies that these cells can adopt a neuronal differentiation pathway in a short time compared with data on other adult stem cells (Bossolasco et al., 2005; Chua et al., 2009), and with more time differentiation into other neural cell lineages will occur.

Bottom Line: The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT-PCR (reverse transcription-PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs.The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry.In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT
Human EnSC (endometrial-derived stem cell) is an abundant and easily available source for cell replacement therapy. Many investigations have shown the potency of the cells to differentiate into several mesoderm-derived cell lineages, including osteocytes and adipocytes. Here, the potency of EnSC in neural differentiation has been investigated. Flow cytometric analysis showed that they were positive for CD90, CD105, OCT4, CD44 and negative for CD31, CD34, CD133. The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT-PCR (reverse transcription-PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs. The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry. In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

No MeSH data available.


Related in: MedlinePlus