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Human endometrial stem cells as a new source for programming to neural cells.

Mobarakeh ZT, Ai J, Yazdani F, Sorkhabadi SM, Ghanbari Z, Javidan AN, Mortazavi-Tabatabaei SA, Massumi M, Barough SE - Cell Biol Int Rep (2010) (2012)

Bottom Line: The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT-PCR (reverse transcription-PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs.The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry.In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT
Human EnSC (endometrial-derived stem cell) is an abundant and easily available source for cell replacement therapy. Many investigations have shown the potency of the cells to differentiate into several mesoderm-derived cell lineages, including osteocytes and adipocytes. Here, the potency of EnSC in neural differentiation has been investigated. Flow cytometric analysis showed that they were positive for CD90, CD105, OCT4, CD44 and negative for CD31, CD34, CD133. The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT-PCR (reverse transcription-PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs. The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry. In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

No MeSH data available.


Related in: MedlinePlus

Immunocytochemical analysis for expression of MAP2, β3-tub, NF-L and Olig1 as markers of mature neurons and oligodendrocyte in EnSC before neuronal induction (A′′, B′′, C′′and D′′) and 12 days PT by neuronal inducing signalling molecules (A′, B′ and C′)Nuclei were stained with DAPI (A, B and C). Scale bar: 100 μm.
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Figure 5: Immunocytochemical analysis for expression of MAP2, β3-tub, NF-L and Olig1 as markers of mature neurons and oligodendrocyte in EnSC before neuronal induction (A′′, B′′, C′′and D′′) and 12 days PT by neuronal inducing signalling molecules (A′, B′ and C′)Nuclei were stained with DAPI (A, B and C). Scale bar: 100 μm.

Mentions: To correlate the morphological changes with neuronal differentiation, we analysed the expression of neuron-specific markers in differentiated neuronal-like cells by immunocytochemistry. Neuronal cells are classically characterized by the expression of cytoskeletal proteins that include NF-L, β3-tub, MAP2 and olig1, which are expressed in mature neurons. Figure 4 shows the expression of these mature markers in neuronal-like cell differentiated from EnSC only after induction (Figures 5A′, 5B′, 5C′ and 5D′) by signalling molecules, while they were not expressed in EnSC before neuronal differentiation (Figures 5A″, 5B″, 5C″ and 5D″). Thus EnSC can respond to the signalling molecules, differentiate into neurons and express the mature neuronal markers.


Human endometrial stem cells as a new source for programming to neural cells.

Mobarakeh ZT, Ai J, Yazdani F, Sorkhabadi SM, Ghanbari Z, Javidan AN, Mortazavi-Tabatabaei SA, Massumi M, Barough SE - Cell Biol Int Rep (2010) (2012)

Immunocytochemical analysis for expression of MAP2, β3-tub, NF-L and Olig1 as markers of mature neurons and oligodendrocyte in EnSC before neuronal induction (A′′, B′′, C′′and D′′) and 12 days PT by neuronal inducing signalling molecules (A′, B′ and C′)Nuclei were stained with DAPI (A, B and C). Scale bar: 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475442&req=5

Figure 5: Immunocytochemical analysis for expression of MAP2, β3-tub, NF-L and Olig1 as markers of mature neurons and oligodendrocyte in EnSC before neuronal induction (A′′, B′′, C′′and D′′) and 12 days PT by neuronal inducing signalling molecules (A′, B′ and C′)Nuclei were stained with DAPI (A, B and C). Scale bar: 100 μm.
Mentions: To correlate the morphological changes with neuronal differentiation, we analysed the expression of neuron-specific markers in differentiated neuronal-like cells by immunocytochemistry. Neuronal cells are classically characterized by the expression of cytoskeletal proteins that include NF-L, β3-tub, MAP2 and olig1, which are expressed in mature neurons. Figure 4 shows the expression of these mature markers in neuronal-like cell differentiated from EnSC only after induction (Figures 5A′, 5B′, 5C′ and 5D′) by signalling molecules, while they were not expressed in EnSC before neuronal differentiation (Figures 5A″, 5B″, 5C″ and 5D″). Thus EnSC can respond to the signalling molecules, differentiate into neurons and express the mature neuronal markers.

Bottom Line: The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT-PCR (reverse transcription-PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs.The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry.In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT
Human EnSC (endometrial-derived stem cell) is an abundant and easily available source for cell replacement therapy. Many investigations have shown the potency of the cells to differentiate into several mesoderm-derived cell lineages, including osteocytes and adipocytes. Here, the potency of EnSC in neural differentiation has been investigated. Flow cytometric analysis showed that they were positive for CD90, CD105, OCT4, CD44 and negative for CD31, CD34, CD133. The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT-PCR (reverse transcription-PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs. The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry. In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

No MeSH data available.


Related in: MedlinePlus