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Human endometrial stem cells as a new source for programming to neural cells.

Mobarakeh ZT, Ai J, Yazdani F, Sorkhabadi SM, Ghanbari Z, Javidan AN, Mortazavi-Tabatabaei SA, Massumi M, Barough SE - Cell Biol Int Rep (2010) (2012)

Bottom Line: The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT-PCR (reverse transcription-PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs.The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry.In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT
Human EnSC (endometrial-derived stem cell) is an abundant and easily available source for cell replacement therapy. Many investigations have shown the potency of the cells to differentiate into several mesoderm-derived cell lineages, including osteocytes and adipocytes. Here, the potency of EnSC in neural differentiation has been investigated. Flow cytometric analysis showed that they were positive for CD90, CD105, OCT4, CD44 and negative for CD31, CD34, CD133. The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT-PCR (reverse transcription-PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs. The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry. In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

No MeSH data available.


Related in: MedlinePlus

Human EnSC 7 and 12 days PT by neural inducing signalling molecules(A–C) Neuronal-like cells derived from EnSC 7 days PT. (D–F) Differentiated cells 12 days PT with a clear morphological neuronal shape (consisting of b-fibre-bearing cells with features typical of cultured neurons, that is sharply defined, phase-bright bodies, and thin, long, often branching processes (arrows show branching processes). Scale bar: 100 μm.
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Figure 4: Human EnSC 7 and 12 days PT by neural inducing signalling molecules(A–C) Neuronal-like cells derived from EnSC 7 days PT. (D–F) Differentiated cells 12 days PT with a clear morphological neuronal shape (consisting of b-fibre-bearing cells with features typical of cultured neurons, that is sharply defined, phase-bright bodies, and thin, long, often branching processes (arrows show branching processes). Scale bar: 100 μm.

Mentions: To induce neural differentiation of human EnSC in long-term cultures, the cells were cultured in the classical serum-free medium (DMEM/F12 supplemented with N2) and induced by bFGF, PDGF and EGF signalling molecules, which classically can lead to neural differentiation. To identify neuro-glial differentiation, the differentiating cells were observed daily by phase-contrast microscopy (Figure 4). After 7 days exposure to neural inducing signalling molecules, changes in the shape of most cells were observed (Figures 4A–4C). Some short neurite-like extensions were obvious after 7 days PT, and easily recognizable and fully developed after 12 days PT (Figures 4D–4F). Initially, cytoplasm in EnSC retracted towards the nucleus, forming a contracted multipolar cell body and leaving some process-like extensions peripherally. The cell bodies became increasingly pyramidal or spherical, and refractile, exhibiting a typical neuronal perikaryal appearance. The cell process became longer and more evident (arrows show branching processes in all of Figures).


Human endometrial stem cells as a new source for programming to neural cells.

Mobarakeh ZT, Ai J, Yazdani F, Sorkhabadi SM, Ghanbari Z, Javidan AN, Mortazavi-Tabatabaei SA, Massumi M, Barough SE - Cell Biol Int Rep (2010) (2012)

Human EnSC 7 and 12 days PT by neural inducing signalling molecules(A–C) Neuronal-like cells derived from EnSC 7 days PT. (D–F) Differentiated cells 12 days PT with a clear morphological neuronal shape (consisting of b-fibre-bearing cells with features typical of cultured neurons, that is sharply defined, phase-bright bodies, and thin, long, often branching processes (arrows show branching processes). Scale bar: 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475442&req=5

Figure 4: Human EnSC 7 and 12 days PT by neural inducing signalling molecules(A–C) Neuronal-like cells derived from EnSC 7 days PT. (D–F) Differentiated cells 12 days PT with a clear morphological neuronal shape (consisting of b-fibre-bearing cells with features typical of cultured neurons, that is sharply defined, phase-bright bodies, and thin, long, often branching processes (arrows show branching processes). Scale bar: 100 μm.
Mentions: To induce neural differentiation of human EnSC in long-term cultures, the cells were cultured in the classical serum-free medium (DMEM/F12 supplemented with N2) and induced by bFGF, PDGF and EGF signalling molecules, which classically can lead to neural differentiation. To identify neuro-glial differentiation, the differentiating cells were observed daily by phase-contrast microscopy (Figure 4). After 7 days exposure to neural inducing signalling molecules, changes in the shape of most cells were observed (Figures 4A–4C). Some short neurite-like extensions were obvious after 7 days PT, and easily recognizable and fully developed after 12 days PT (Figures 4D–4F). Initially, cytoplasm in EnSC retracted towards the nucleus, forming a contracted multipolar cell body and leaving some process-like extensions peripherally. The cell bodies became increasingly pyramidal or spherical, and refractile, exhibiting a typical neuronal perikaryal appearance. The cell process became longer and more evident (arrows show branching processes in all of Figures).

Bottom Line: The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT-PCR (reverse transcription-PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs.The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry.In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT
Human EnSC (endometrial-derived stem cell) is an abundant and easily available source for cell replacement therapy. Many investigations have shown the potency of the cells to differentiate into several mesoderm-derived cell lineages, including osteocytes and adipocytes. Here, the potency of EnSC in neural differentiation has been investigated. Flow cytometric analysis showed that they were positive for CD90, CD105, OCT4, CD44 and negative for CD31, CD34, CD133. The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT-PCR (reverse transcription-PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs. The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry. In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

No MeSH data available.


Related in: MedlinePlus