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Human endometrial stem cells as a new source for programming to neural cells.

Mobarakeh ZT, Ai J, Yazdani F, Sorkhabadi SM, Ghanbari Z, Javidan AN, Mortazavi-Tabatabaei SA, Massumi M, Barough SE - Cell Biol Int Rep (2010) (2012)

Bottom Line: The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT-PCR (reverse transcription-PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs.The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry.In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT
Human EnSC (endometrial-derived stem cell) is an abundant and easily available source for cell replacement therapy. Many investigations have shown the potency of the cells to differentiate into several mesoderm-derived cell lineages, including osteocytes and adipocytes. Here, the potency of EnSC in neural differentiation has been investigated. Flow cytometric analysis showed that they were positive for CD90, CD105, OCT4, CD44 and negative for CD31, CD34, CD133. The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT-PCR (reverse transcription-PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs. The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry. In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

No MeSH data available.


Related in: MedlinePlus

Flow cytometric analysis of isolated EnSC for mesenchymal stem cell markers (CD90, CD105 and CD44), haemopoietic marker (CD34 and CD133), endothelial marker (CD31) and ES cell marker (OCT4)As shown the isolated cells are positive for CD90, CD105, CD44 and OCT4 and are negative for CD31, CD34 and CD133.
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Figure 2: Flow cytometric analysis of isolated EnSC for mesenchymal stem cell markers (CD90, CD105 and CD44), haemopoietic marker (CD34 and CD133), endothelial marker (CD31) and ES cell marker (OCT4)As shown the isolated cells are positive for CD90, CD105, CD44 and OCT4 and are negative for CD31, CD34 and CD133.

Mentions: The immunophenotype was based on the flow cytometric analysis of a subset of ES cell marker (OCT4), MSC markers (CD90, CD105 and CD44), haemopoietic markers (CD34 and CD133) and endothelial marker (CD31). The analysis showed that they were positive for CD90, CD105 and OCT4 and negative for CD31 and CD34 (Figure 2). Given the phenotypic, morphological, proliferative characteristics of the EnSC, we determined whether these cells were capable of differentiating into various lineages as can other stem cell types. Differentiation into osteocytes and adipocytes was demonstrated by culturing of EnSC using standard commercially available culture reagents and methodologies. Subsequent to treatment with differentiation inducing protocols (Figure 3), EnSC could differentiate into osteocytes and adipocytes.


Human endometrial stem cells as a new source for programming to neural cells.

Mobarakeh ZT, Ai J, Yazdani F, Sorkhabadi SM, Ghanbari Z, Javidan AN, Mortazavi-Tabatabaei SA, Massumi M, Barough SE - Cell Biol Int Rep (2010) (2012)

Flow cytometric analysis of isolated EnSC for mesenchymal stem cell markers (CD90, CD105 and CD44), haemopoietic marker (CD34 and CD133), endothelial marker (CD31) and ES cell marker (OCT4)As shown the isolated cells are positive for CD90, CD105, CD44 and OCT4 and are negative for CD31, CD34 and CD133.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475442&req=5

Figure 2: Flow cytometric analysis of isolated EnSC for mesenchymal stem cell markers (CD90, CD105 and CD44), haemopoietic marker (CD34 and CD133), endothelial marker (CD31) and ES cell marker (OCT4)As shown the isolated cells are positive for CD90, CD105, CD44 and OCT4 and are negative for CD31, CD34 and CD133.
Mentions: The immunophenotype was based on the flow cytometric analysis of a subset of ES cell marker (OCT4), MSC markers (CD90, CD105 and CD44), haemopoietic markers (CD34 and CD133) and endothelial marker (CD31). The analysis showed that they were positive for CD90, CD105 and OCT4 and negative for CD31 and CD34 (Figure 2). Given the phenotypic, morphological, proliferative characteristics of the EnSC, we determined whether these cells were capable of differentiating into various lineages as can other stem cell types. Differentiation into osteocytes and adipocytes was demonstrated by culturing of EnSC using standard commercially available culture reagents and methodologies. Subsequent to treatment with differentiation inducing protocols (Figure 3), EnSC could differentiate into osteocytes and adipocytes.

Bottom Line: The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT-PCR (reverse transcription-PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs.The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry.In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT
Human EnSC (endometrial-derived stem cell) is an abundant and easily available source for cell replacement therapy. Many investigations have shown the potency of the cells to differentiate into several mesoderm-derived cell lineages, including osteocytes and adipocytes. Here, the potency of EnSC in neural differentiation has been investigated. Flow cytometric analysis showed that they were positive for CD90, CD105, OCT4, CD44 and negative for CD31, CD34, CD133. The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT-PCR (reverse transcription-PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs. The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry. In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

No MeSH data available.


Related in: MedlinePlus