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Isolation and characterization of equine amnion mesenchymal stem cells.

Coli A, Nocchi F, Lamanna R, Iorio M, Lapi S, Urciuoli P, Scatena F, Giannessi E, Stornelli MR, Passeri S - Cell Biol Int Rep (2010) (2011)

Bottom Line: The cells were grown in α-MEM (α-modified minimum essential medium) and the effect of EGF (epidermal growth factor) supplementation was evaluated.EGF supplementation of the culture medium resulted in a significant increase in PD growth parameter and in the formation of bone nodules for the osteogenic differentiation.By immunohistochemistry the amnion tissue shows a positivity for the c-Kit (cluster tyrosine-protein kinase), CD105 and Oct-4 (octamer-binding transcription factor 4) antigens that confirmed the presence of MSCs with embryonic phenotype.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Pathology, University of Pisa, Pisa, Italy.

ABSTRACT
The amnion is a particular tissue whose cells show features of multipotent stem cells proposed for use in cellular therapy and regenerative medicine. From equine amnion collected after the foal birth we have isolated MSCs (mesenchymal stem cells), namely EAMSCs (equine amnion mesenchymal stem cells), from the mesoblastic layer. The cells were grown in α-MEM (α-modified minimum essential medium) and the effect of EGF (epidermal growth factor) supplementation was evaluated. To assess the growth kinetic of EAMSCs we have taken into account some parameters [PD (population doubling), fold increase and DT (doubling time)]. The differentiation in chondrogenic, adipogenic and osteogenic types of cells and their epitope expression by a cytofluorimetric study have been reported. EGF supplementation of the culture medium resulted in a significant increase in PD growth parameter and in the formation of bone nodules for the osteogenic differentiation. By immunohistochemistry the amnion tissue shows a positivity for the c-Kit (cluster tyrosine-protein kinase), CD105 and Oct-4 (octamer-binding transcription factor 4) antigens that confirmed the presence of MSCs with embryonic phenotype.

No MeSH data available.


PD of EAMSCs
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Figure 3: PD of EAMSCs

Mentions: α-MEM supplemented with 10% FCS and 10% HS was an excellent culture medium in terms of expansion and cell passage number (P = 0.005). In addition, EGF added to α-MEM resulted in a significant increase in PD parameter for each step (P = 0.005; Figure 3). In addition (Figure 3), DT was calculated for each medium (with or without supplementation of EGF) and each step showed changes in growth rates in relation to culture medium and step number analysed (Figure 4), but the high S.D. values do not allow a proper statistical analysis.


Isolation and characterization of equine amnion mesenchymal stem cells.

Coli A, Nocchi F, Lamanna R, Iorio M, Lapi S, Urciuoli P, Scatena F, Giannessi E, Stornelli MR, Passeri S - Cell Biol Int Rep (2010) (2011)

PD of EAMSCs
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475441&req=5

Figure 3: PD of EAMSCs
Mentions: α-MEM supplemented with 10% FCS and 10% HS was an excellent culture medium in terms of expansion and cell passage number (P = 0.005). In addition, EGF added to α-MEM resulted in a significant increase in PD parameter for each step (P = 0.005; Figure 3). In addition (Figure 3), DT was calculated for each medium (with or without supplementation of EGF) and each step showed changes in growth rates in relation to culture medium and step number analysed (Figure 4), but the high S.D. values do not allow a proper statistical analysis.

Bottom Line: The cells were grown in α-MEM (α-modified minimum essential medium) and the effect of EGF (epidermal growth factor) supplementation was evaluated.EGF supplementation of the culture medium resulted in a significant increase in PD growth parameter and in the formation of bone nodules for the osteogenic differentiation.By immunohistochemistry the amnion tissue shows a positivity for the c-Kit (cluster tyrosine-protein kinase), CD105 and Oct-4 (octamer-binding transcription factor 4) antigens that confirmed the presence of MSCs with embryonic phenotype.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Pathology, University of Pisa, Pisa, Italy.

ABSTRACT
The amnion is a particular tissue whose cells show features of multipotent stem cells proposed for use in cellular therapy and regenerative medicine. From equine amnion collected after the foal birth we have isolated MSCs (mesenchymal stem cells), namely EAMSCs (equine amnion mesenchymal stem cells), from the mesoblastic layer. The cells were grown in α-MEM (α-modified minimum essential medium) and the effect of EGF (epidermal growth factor) supplementation was evaluated. To assess the growth kinetic of EAMSCs we have taken into account some parameters [PD (population doubling), fold increase and DT (doubling time)]. The differentiation in chondrogenic, adipogenic and osteogenic types of cells and their epitope expression by a cytofluorimetric study have been reported. EGF supplementation of the culture medium resulted in a significant increase in PD growth parameter and in the formation of bone nodules for the osteogenic differentiation. By immunohistochemistry the amnion tissue shows a positivity for the c-Kit (cluster tyrosine-protein kinase), CD105 and Oct-4 (octamer-binding transcription factor 4) antigens that confirmed the presence of MSCs with embryonic phenotype.

No MeSH data available.