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Effects of different elicitors on 10-deacetylbaccatin III-10-O-acetyltransferase activity and cytochrome P450 monooxygenase content in suspension cultures of Taxus cuspidata cells.

Zhang JF, Gong S, Guo ZG - Cell Biol Int Rep (2010) (2011)

Bottom Line: After elicitation, the concentrations of cytochrome P450 monooxygenase were also induced to its maximum values of 0.069, 0.336, 0.321 and 0.193 nmol/ml, respectively.In addition, under the elicitation, the change in 10-DBAT activity was similar to that of cytochrome P450 monooxygenase concentration.The products of these two enzymes changed after the variety of the enzymes, and the taxol content increased through the cultivation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, No.1 QingHuaYuan Street, Haidian District, Beijing, 100084, Peoples Republic of China.

ABSTRACT
The effects of four elicitors, including 100 μmol/l MeJA (methyl jasmonate), 40 μl/l hydrogen peroxide (30%, w/w), 80 mg/l SA (salicylic acid) and 0.4 g/l F3 (fungal elicitor), on suspension cultures of Taxus cuspidata were studied. After addition of the above four elicitors, the enzyme activity of 10-DBAT (10-deacetylbaccatin III-10-O-acetyltransferase) was induced and reached its maximum of 5.47, 0.97, 3.30 and 6.82 U, respectively. After elicitation, the concentrations of cytochrome P450 monooxygenase were also induced to its maximum values of 0.069, 0.336, 0.321 and 0.193 nmol/ml, respectively. In addition, under the elicitation, the change in 10-DBAT activity was similar to that of cytochrome P450 monooxygenase concentration. The products of these two enzymes changed after the variety of the enzymes, and the taxol content increased through the cultivation.

No MeSH data available.


Time course of enzyme activity of 10-DBAT (♦), the concentration of cytochrome P450 monooxygenase (▾), the contents of 10-DAB III (▾), baccatin III (•) and taxol (▪) after the induction of combination of MeJA, SA and hydrogen peroxide elicitor in T. cuspidata cell suspension cultureSamples were taken 0, 8, 16, 24, 48, 72 and 96 h after the induction. Values are means of triplicate results, and error bars represent the S.D.
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Figure 5: Time course of enzyme activity of 10-DBAT (♦), the concentration of cytochrome P450 monooxygenase (▾), the contents of 10-DAB III (▾), baccatin III (•) and taxol (▪) after the induction of combination of MeJA, SA and hydrogen peroxide elicitor in T. cuspidata cell suspension cultureSamples were taken 0, 8, 16, 24, 48, 72 and 96 h after the induction. Values are means of triplicate results, and error bars represent the S.D.

Mentions: The effects of the combination of the elicitors of MeJA, SA and hydrogen peroxide on the enzyme and production of taxanes (Khosroushahi et al., 2006) are shown (Figures 5A, 5B). Though the concentration of cytochrome P450 monooxygenase changed similarly to those when a single elicitor of MeJA and F3 was added, the absolute value was lower. The activity of 10-DBAT reached a maximum value by 24 h ,which was similar to the SA elicitation, and also the absolute value of the combination of the elicitors was lower than those under the elicitation of MeJA and F3. This may be because of the offset effect when blending several elicitors.


Effects of different elicitors on 10-deacetylbaccatin III-10-O-acetyltransferase activity and cytochrome P450 monooxygenase content in suspension cultures of Taxus cuspidata cells.

Zhang JF, Gong S, Guo ZG - Cell Biol Int Rep (2010) (2011)

Time course of enzyme activity of 10-DBAT (♦), the concentration of cytochrome P450 monooxygenase (▾), the contents of 10-DAB III (▾), baccatin III (•) and taxol (▪) after the induction of combination of MeJA, SA and hydrogen peroxide elicitor in T. cuspidata cell suspension cultureSamples were taken 0, 8, 16, 24, 48, 72 and 96 h after the induction. Values are means of triplicate results, and error bars represent the S.D.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475439&req=5

Figure 5: Time course of enzyme activity of 10-DBAT (♦), the concentration of cytochrome P450 monooxygenase (▾), the contents of 10-DAB III (▾), baccatin III (•) and taxol (▪) after the induction of combination of MeJA, SA and hydrogen peroxide elicitor in T. cuspidata cell suspension cultureSamples were taken 0, 8, 16, 24, 48, 72 and 96 h after the induction. Values are means of triplicate results, and error bars represent the S.D.
Mentions: The effects of the combination of the elicitors of MeJA, SA and hydrogen peroxide on the enzyme and production of taxanes (Khosroushahi et al., 2006) are shown (Figures 5A, 5B). Though the concentration of cytochrome P450 monooxygenase changed similarly to those when a single elicitor of MeJA and F3 was added, the absolute value was lower. The activity of 10-DBAT reached a maximum value by 24 h ,which was similar to the SA elicitation, and also the absolute value of the combination of the elicitors was lower than those under the elicitation of MeJA and F3. This may be because of the offset effect when blending several elicitors.

Bottom Line: After elicitation, the concentrations of cytochrome P450 monooxygenase were also induced to its maximum values of 0.069, 0.336, 0.321 and 0.193 nmol/ml, respectively.In addition, under the elicitation, the change in 10-DBAT activity was similar to that of cytochrome P450 monooxygenase concentration.The products of these two enzymes changed after the variety of the enzymes, and the taxol content increased through the cultivation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, No.1 QingHuaYuan Street, Haidian District, Beijing, 100084, Peoples Republic of China.

ABSTRACT
The effects of four elicitors, including 100 μmol/l MeJA (methyl jasmonate), 40 μl/l hydrogen peroxide (30%, w/w), 80 mg/l SA (salicylic acid) and 0.4 g/l F3 (fungal elicitor), on suspension cultures of Taxus cuspidata were studied. After addition of the above four elicitors, the enzyme activity of 10-DBAT (10-deacetylbaccatin III-10-O-acetyltransferase) was induced and reached its maximum of 5.47, 0.97, 3.30 and 6.82 U, respectively. After elicitation, the concentrations of cytochrome P450 monooxygenase were also induced to its maximum values of 0.069, 0.336, 0.321 and 0.193 nmol/ml, respectively. In addition, under the elicitation, the change in 10-DBAT activity was similar to that of cytochrome P450 monooxygenase concentration. The products of these two enzymes changed after the variety of the enzymes, and the taxol content increased through the cultivation.

No MeSH data available.