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Effects of different elicitors on 10-deacetylbaccatin III-10-O-acetyltransferase activity and cytochrome P450 monooxygenase content in suspension cultures of Taxus cuspidata cells.

Zhang JF, Gong S, Guo ZG - Cell Biol Int Rep (2010) (2011)

Bottom Line: After elicitation, the concentrations of cytochrome P450 monooxygenase were also induced to its maximum values of 0.069, 0.336, 0.321 and 0.193 nmol/ml, respectively.In addition, under the elicitation, the change in 10-DBAT activity was similar to that of cytochrome P450 monooxygenase concentration.The products of these two enzymes changed after the variety of the enzymes, and the taxol content increased through the cultivation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, No.1 QingHuaYuan Street, Haidian District, Beijing, 100084, Peoples Republic of China.

ABSTRACT
The effects of four elicitors, including 100 μmol/l MeJA (methyl jasmonate), 40 μl/l hydrogen peroxide (30%, w/w), 80 mg/l SA (salicylic acid) and 0.4 g/l F3 (fungal elicitor), on suspension cultures of Taxus cuspidata were studied. After addition of the above four elicitors, the enzyme activity of 10-DBAT (10-deacetylbaccatin III-10-O-acetyltransferase) was induced and reached its maximum of 5.47, 0.97, 3.30 and 6.82 U, respectively. After elicitation, the concentrations of cytochrome P450 monooxygenase were also induced to its maximum values of 0.069, 0.336, 0.321 and 0.193 nmol/ml, respectively. In addition, under the elicitation, the change in 10-DBAT activity was similar to that of cytochrome P450 monooxygenase concentration. The products of these two enzymes changed after the variety of the enzymes, and the taxol content increased through the cultivation.

No MeSH data available.


Time course of enzyme activity of 10-DBAT (♦), the concentration of cytochrome P450 monooxygenase (▾), the contents of 10-DAB III (▾), baccatin III (•) and taxol (▪) after the induction with 80 mg/l SA in T. cuspidata cell suspension cultureSamples were taken 0, 8, 16, 24, 48, 72 and 96 h after the induction. Values are means of triplicate results, and error bars represent the S.D.
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Figure 3: Time course of enzyme activity of 10-DBAT (♦), the concentration of cytochrome P450 monooxygenase (▾), the contents of 10-DAB III (▾), baccatin III (•) and taxol (▪) after the induction with 80 mg/l SA in T. cuspidata cell suspension cultureSamples were taken 0, 8, 16, 24, 48, 72 and 96 h after the induction. Values are means of triplicate results, and error bars represent the S.D.

Mentions: SA was added as an elicitor to improve Taxol production (Yu et al., 2001; Wang et al., 2007). Our previous experiment showed that the optimal concentration of SA was 80 mg/l. The results of the SA elicitation experiment is shown (Figures 3A, 3B). After the addition of SA, both cytochrome P450 monooxygenase and 10-DBAT immediately began to increase rapidly and reached the respective maximal values of 0.321 nmol/ml and 3.30 U at the 16th and 24th hours, respectively. After that, both of them started to decline, and cytochrome P450 monooxygenase declined more quickly.


Effects of different elicitors on 10-deacetylbaccatin III-10-O-acetyltransferase activity and cytochrome P450 monooxygenase content in suspension cultures of Taxus cuspidata cells.

Zhang JF, Gong S, Guo ZG - Cell Biol Int Rep (2010) (2011)

Time course of enzyme activity of 10-DBAT (♦), the concentration of cytochrome P450 monooxygenase (▾), the contents of 10-DAB III (▾), baccatin III (•) and taxol (▪) after the induction with 80 mg/l SA in T. cuspidata cell suspension cultureSamples were taken 0, 8, 16, 24, 48, 72 and 96 h after the induction. Values are means of triplicate results, and error bars represent the S.D.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475439&req=5

Figure 3: Time course of enzyme activity of 10-DBAT (♦), the concentration of cytochrome P450 monooxygenase (▾), the contents of 10-DAB III (▾), baccatin III (•) and taxol (▪) after the induction with 80 mg/l SA in T. cuspidata cell suspension cultureSamples were taken 0, 8, 16, 24, 48, 72 and 96 h after the induction. Values are means of triplicate results, and error bars represent the S.D.
Mentions: SA was added as an elicitor to improve Taxol production (Yu et al., 2001; Wang et al., 2007). Our previous experiment showed that the optimal concentration of SA was 80 mg/l. The results of the SA elicitation experiment is shown (Figures 3A, 3B). After the addition of SA, both cytochrome P450 monooxygenase and 10-DBAT immediately began to increase rapidly and reached the respective maximal values of 0.321 nmol/ml and 3.30 U at the 16th and 24th hours, respectively. After that, both of them started to decline, and cytochrome P450 monooxygenase declined more quickly.

Bottom Line: After elicitation, the concentrations of cytochrome P450 monooxygenase were also induced to its maximum values of 0.069, 0.336, 0.321 and 0.193 nmol/ml, respectively.In addition, under the elicitation, the change in 10-DBAT activity was similar to that of cytochrome P450 monooxygenase concentration.The products of these two enzymes changed after the variety of the enzymes, and the taxol content increased through the cultivation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, No.1 QingHuaYuan Street, Haidian District, Beijing, 100084, Peoples Republic of China.

ABSTRACT
The effects of four elicitors, including 100 μmol/l MeJA (methyl jasmonate), 40 μl/l hydrogen peroxide (30%, w/w), 80 mg/l SA (salicylic acid) and 0.4 g/l F3 (fungal elicitor), on suspension cultures of Taxus cuspidata were studied. After addition of the above four elicitors, the enzyme activity of 10-DBAT (10-deacetylbaccatin III-10-O-acetyltransferase) was induced and reached its maximum of 5.47, 0.97, 3.30 and 6.82 U, respectively. After elicitation, the concentrations of cytochrome P450 monooxygenase were also induced to its maximum values of 0.069, 0.336, 0.321 and 0.193 nmol/ml, respectively. In addition, under the elicitation, the change in 10-DBAT activity was similar to that of cytochrome P450 monooxygenase concentration. The products of these two enzymes changed after the variety of the enzymes, and the taxol content increased through the cultivation.

No MeSH data available.