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Effects of different elicitors on 10-deacetylbaccatin III-10-O-acetyltransferase activity and cytochrome P450 monooxygenase content in suspension cultures of Taxus cuspidata cells.

Zhang JF, Gong S, Guo ZG - Cell Biol Int Rep (2010) (2011)

Bottom Line: After elicitation, the concentrations of cytochrome P450 monooxygenase were also induced to its maximum values of 0.069, 0.336, 0.321 and 0.193 nmol/ml, respectively.In addition, under the elicitation, the change in 10-DBAT activity was similar to that of cytochrome P450 monooxygenase concentration.The products of these two enzymes changed after the variety of the enzymes, and the taxol content increased through the cultivation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, No.1 QingHuaYuan Street, Haidian District, Beijing, 100084, Peoples Republic of China.

ABSTRACT
The effects of four elicitors, including 100 μmol/l MeJA (methyl jasmonate), 40 μl/l hydrogen peroxide (30%, w/w), 80 mg/l SA (salicylic acid) and 0.4 g/l F3 (fungal elicitor), on suspension cultures of Taxus cuspidata were studied. After addition of the above four elicitors, the enzyme activity of 10-DBAT (10-deacetylbaccatin III-10-O-acetyltransferase) was induced and reached its maximum of 5.47, 0.97, 3.30 and 6.82 U, respectively. After elicitation, the concentrations of cytochrome P450 monooxygenase were also induced to its maximum values of 0.069, 0.336, 0.321 and 0.193 nmol/ml, respectively. In addition, under the elicitation, the change in 10-DBAT activity was similar to that of cytochrome P450 monooxygenase concentration. The products of these two enzymes changed after the variety of the enzymes, and the taxol content increased through the cultivation.

No MeSH data available.


Time course of enzyme activity of 10-DBAT (♦), the concentration of cytochrome P450 monooxygenase (▾), the contents of 10-DAB III (▾), baccatin III (•) and Taxol (▪) after the induction with 100 μM MeJA, in T. cuspidata cell suspension cultureSamples were taken 0, 8, 16, 24, 48, 72 and 96 h after the induction. Values are means of triplicate results and error bars represent the S.D.
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Figure 1: Time course of enzyme activity of 10-DBAT (♦), the concentration of cytochrome P450 monooxygenase (▾), the contents of 10-DAB III (▾), baccatin III (•) and Taxol (▪) after the induction with 100 μM MeJA, in T. cuspidata cell suspension cultureSamples were taken 0, 8, 16, 24, 48, 72 and 96 h after the induction. Values are means of triplicate results and error bars represent the S.D.

Mentions: MeJA was an important elicitor found to increase taxol production in Taxus cell cultures effectively (Mirjalili and Linden, 1996; Yukimune et al., 1996; Wang and Wu, 2005). Our previous experiment showed that 100μM MeJA was the optimum concentration for elicitation of taxol production. The results of the enzyme activity of 10-DBAT and contents of cytochrome P450 monooxygenase, taxol, baccatin III and 10-DAB III after the addition of 100 μmol/l MeJA into the culture are shown (Figures 1A, 1B). After inoculating the cells into the induction medium, the concentration of cytochrome P450 monooxygenase increased quickly on the addition of MeJA. At the 48th hour, it reached maximal value and then decreased quickly. At the beginning of the experiment, the activity of 10-DBAT increased slowly. The enzyme activity of 10-DBAT began to increase rapidly at the 24th hour and reached a maximum of 5.47 U at the 48th hour, then decreased to near the level before the elicitation at the 96th hour (Figure 1A).


Effects of different elicitors on 10-deacetylbaccatin III-10-O-acetyltransferase activity and cytochrome P450 monooxygenase content in suspension cultures of Taxus cuspidata cells.

Zhang JF, Gong S, Guo ZG - Cell Biol Int Rep (2010) (2011)

Time course of enzyme activity of 10-DBAT (♦), the concentration of cytochrome P450 monooxygenase (▾), the contents of 10-DAB III (▾), baccatin III (•) and Taxol (▪) after the induction with 100 μM MeJA, in T. cuspidata cell suspension cultureSamples were taken 0, 8, 16, 24, 48, 72 and 96 h after the induction. Values are means of triplicate results and error bars represent the S.D.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475439&req=5

Figure 1: Time course of enzyme activity of 10-DBAT (♦), the concentration of cytochrome P450 monooxygenase (▾), the contents of 10-DAB III (▾), baccatin III (•) and Taxol (▪) after the induction with 100 μM MeJA, in T. cuspidata cell suspension cultureSamples were taken 0, 8, 16, 24, 48, 72 and 96 h after the induction. Values are means of triplicate results and error bars represent the S.D.
Mentions: MeJA was an important elicitor found to increase taxol production in Taxus cell cultures effectively (Mirjalili and Linden, 1996; Yukimune et al., 1996; Wang and Wu, 2005). Our previous experiment showed that 100μM MeJA was the optimum concentration for elicitation of taxol production. The results of the enzyme activity of 10-DBAT and contents of cytochrome P450 monooxygenase, taxol, baccatin III and 10-DAB III after the addition of 100 μmol/l MeJA into the culture are shown (Figures 1A, 1B). After inoculating the cells into the induction medium, the concentration of cytochrome P450 monooxygenase increased quickly on the addition of MeJA. At the 48th hour, it reached maximal value and then decreased quickly. At the beginning of the experiment, the activity of 10-DBAT increased slowly. The enzyme activity of 10-DBAT began to increase rapidly at the 24th hour and reached a maximum of 5.47 U at the 48th hour, then decreased to near the level before the elicitation at the 96th hour (Figure 1A).

Bottom Line: After elicitation, the concentrations of cytochrome P450 monooxygenase were also induced to its maximum values of 0.069, 0.336, 0.321 and 0.193 nmol/ml, respectively.In addition, under the elicitation, the change in 10-DBAT activity was similar to that of cytochrome P450 monooxygenase concentration.The products of these two enzymes changed after the variety of the enzymes, and the taxol content increased through the cultivation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, No.1 QingHuaYuan Street, Haidian District, Beijing, 100084, Peoples Republic of China.

ABSTRACT
The effects of four elicitors, including 100 μmol/l MeJA (methyl jasmonate), 40 μl/l hydrogen peroxide (30%, w/w), 80 mg/l SA (salicylic acid) and 0.4 g/l F3 (fungal elicitor), on suspension cultures of Taxus cuspidata were studied. After addition of the above four elicitors, the enzyme activity of 10-DBAT (10-deacetylbaccatin III-10-O-acetyltransferase) was induced and reached its maximum of 5.47, 0.97, 3.30 and 6.82 U, respectively. After elicitation, the concentrations of cytochrome P450 monooxygenase were also induced to its maximum values of 0.069, 0.336, 0.321 and 0.193 nmol/ml, respectively. In addition, under the elicitation, the change in 10-DBAT activity was similar to that of cytochrome P450 monooxygenase concentration. The products of these two enzymes changed after the variety of the enzymes, and the taxol content increased through the cultivation.

No MeSH data available.