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Establishment of glass catfish (Kryptopterus bicirrhis) fin-derived cells.

Han JE, Choresca CH, Koo OJ, Oh HJ, Hong SG, Kim JH, Shin SP, Jun JW, Lee BC, Park SC - Cell Biol Int Rep (2010) (2011)

Bottom Line: The cells grew well in DMEM (Dulbecco's modified Eagle's medium) containing 1% (v/v) P/S (penicillin-streptomycin) and 10% (v/v) fetal bovine serum at 26°C and showed increased cryopreservation efficiency with the slow-freezing method in the presence of 15% dimethyl sulfoxide.In addition, cell cycle analysis was evaluated based on flow cytometric analysis, and culturing to confluence (>85%) was more effective for synchronizing cells at the G(0)/G(1) stages than roscovitine treatment (<75%).The results from testing the cell's viability following cryopreservation and subjecting the cells to cycle analysis can be useful tools for genetic resource management.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Aquatic Animal Medicine.

ABSTRACT
Genetically manipulated transparent animals were already explored in many species for in vivo study of gene expression, transplantation analysis and cancer biology. However, there are no reports about transparent animals as in vitro genetic resources. In the present study, fin-derived cells from glass catfish (Krytopterus bicirrhis), naturally transparent fish with a visible skeleton and internal organs, were isolated after culturing fin explants and characterized using cryopreservation and cell cycle analysis. The cells grew well in DMEM (Dulbecco's modified Eagle's medium) containing 1% (v/v) P/S (penicillin-streptomycin) and 10% (v/v) fetal bovine serum at 26°C and showed increased cryopreservation efficiency with the slow-freezing method in the presence of 15% dimethyl sulfoxide. In addition, cell cycle analysis was evaluated based on flow cytometric analysis, and culturing to confluence (>85%) was more effective for synchronizing cells at the G(0)/G(1) stages than roscovitine treatment (<75%). This is the first report about cell isolation from transparent animals. The results from testing the cell's viability following cryopreservation and subjecting the cells to cycle analysis can be useful tools for genetic resource management.

No MeSH data available.


Related in: MedlinePlus

Photomicrograph of cultured cells from glass catfish and the growth rate comparison of three fintypes (caudal, pectoral and abdominal)(A) Monolayer formation from pectoral fin at passage seven, scale bar = 0.2 mm. (B) Scale bar = 80 μm. (C) Scale bar = 40 μm. (D) Single cell from pectoral fin at passage seven, scale bar = 40 μm. (E) Differences in growth rate among three fin types (means±S.E.M.).
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Figure 1: Photomicrograph of cultured cells from glass catfish and the growth rate comparison of three fintypes (caudal, pectoral and abdominal)(A) Monolayer formation from pectoral fin at passage seven, scale bar = 0.2 mm. (B) Scale bar = 80 μm. (C) Scale bar = 40 μm. (D) Single cell from pectoral fin at passage seven, scale bar = 40 μm. (E) Differences in growth rate among three fin types (means±S.E.M.).

Mentions: Cells derived from the caudal, abdominal and pectoral fins have reached confluency within 2 to 3 weeks (data not shown). DMEM supplemented with 10% FBS (v/v) was determined to be the optimal growth medium, and 26°C was the best temperature for maintaining the primary cell cultures. Additionally, the cell cultures could be maintained for 13 to 15 days without medium change. Photomicrographs of cultured cells at passage seven are shown (Figures 1A to 1D).


Establishment of glass catfish (Kryptopterus bicirrhis) fin-derived cells.

Han JE, Choresca CH, Koo OJ, Oh HJ, Hong SG, Kim JH, Shin SP, Jun JW, Lee BC, Park SC - Cell Biol Int Rep (2010) (2011)

Photomicrograph of cultured cells from glass catfish and the growth rate comparison of three fintypes (caudal, pectoral and abdominal)(A) Monolayer formation from pectoral fin at passage seven, scale bar = 0.2 mm. (B) Scale bar = 80 μm. (C) Scale bar = 40 μm. (D) Single cell from pectoral fin at passage seven, scale bar = 40 μm. (E) Differences in growth rate among three fin types (means±S.E.M.).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475438&req=5

Figure 1: Photomicrograph of cultured cells from glass catfish and the growth rate comparison of three fintypes (caudal, pectoral and abdominal)(A) Monolayer formation from pectoral fin at passage seven, scale bar = 0.2 mm. (B) Scale bar = 80 μm. (C) Scale bar = 40 μm. (D) Single cell from pectoral fin at passage seven, scale bar = 40 μm. (E) Differences in growth rate among three fin types (means±S.E.M.).
Mentions: Cells derived from the caudal, abdominal and pectoral fins have reached confluency within 2 to 3 weeks (data not shown). DMEM supplemented with 10% FBS (v/v) was determined to be the optimal growth medium, and 26°C was the best temperature for maintaining the primary cell cultures. Additionally, the cell cultures could be maintained for 13 to 15 days without medium change. Photomicrographs of cultured cells at passage seven are shown (Figures 1A to 1D).

Bottom Line: The cells grew well in DMEM (Dulbecco's modified Eagle's medium) containing 1% (v/v) P/S (penicillin-streptomycin) and 10% (v/v) fetal bovine serum at 26°C and showed increased cryopreservation efficiency with the slow-freezing method in the presence of 15% dimethyl sulfoxide.In addition, cell cycle analysis was evaluated based on flow cytometric analysis, and culturing to confluence (>85%) was more effective for synchronizing cells at the G(0)/G(1) stages than roscovitine treatment (<75%).The results from testing the cell's viability following cryopreservation and subjecting the cells to cycle analysis can be useful tools for genetic resource management.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Aquatic Animal Medicine.

ABSTRACT
Genetically manipulated transparent animals were already explored in many species for in vivo study of gene expression, transplantation analysis and cancer biology. However, there are no reports about transparent animals as in vitro genetic resources. In the present study, fin-derived cells from glass catfish (Krytopterus bicirrhis), naturally transparent fish with a visible skeleton and internal organs, were isolated after culturing fin explants and characterized using cryopreservation and cell cycle analysis. The cells grew well in DMEM (Dulbecco's modified Eagle's medium) containing 1% (v/v) P/S (penicillin-streptomycin) and 10% (v/v) fetal bovine serum at 26°C and showed increased cryopreservation efficiency with the slow-freezing method in the presence of 15% dimethyl sulfoxide. In addition, cell cycle analysis was evaluated based on flow cytometric analysis, and culturing to confluence (>85%) was more effective for synchronizing cells at the G(0)/G(1) stages than roscovitine treatment (<75%). This is the first report about cell isolation from transparent animals. The results from testing the cell's viability following cryopreservation and subjecting the cells to cycle analysis can be useful tools for genetic resource management.

No MeSH data available.


Related in: MedlinePlus