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Down-regulation of TERE1/UBIAD1 activated Ras-MAPK signalling and induced cell proliferation.

Xia Y, Wei X, Wu S, Wang B, Wang X, Hong L - Cell Biol Int Rep (2010) (2010)

Bottom Line: It was found that decreased TERE1/UBIAD1 expression is closely related to both an increased hTERT expression and activation of Ras-MAPK signalling.When the levels of hTERT expression and ERK phosphorylation were measured, it was found that both of them increased in the above transfected cells, suggesting the activation of Ras-MAPK signalling.Our result is the initial demonstration that down-regulation of TERE1 activates Ras-MAPK signalling and induces subsequent cell proliferation.

View Article: PubMed Central - PubMed

Affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Peoples Republic of China ; †Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Peoples Republic of China.

ABSTRACT
TERE1/UBIAD1 is involved in SCCD (Schnyder crystalline corneal dystrophy) and multiple human cancers. So far, the molecular mechanism of TERE1/UBIAD1 in tumourigenesis is unclear. Here, the expression levels of hTERT and TERE1/UBIAD1 in pathologically proven Chinese TCC (transitional cell carcinoma) samples were measured. It was found that decreased TERE1/UBIAD1 expression is closely related to both an increased hTERT expression and activation of Ras-MAPK signalling. Chemically modified TERE1 siRNA oligos were used to knock down TERE1 expression in human L02 cells. Cells transfected with TERE1 siRNA oligos underwent significant cell proliferation. When the levels of hTERT expression and ERK phosphorylation were measured, it was found that both of them increased in the above transfected cells, suggesting the activation of Ras-MAPK signalling. Addition of the MEK inhibitor U0126 into the transfected L02 cells described above inhibited ERK phosphorylation and hTERT expression. Our result is the initial demonstration that down-regulation of TERE1 activates Ras-MAPK signalling and induces subsequent cell proliferation. TERE1 might be a new negative regulator of Ras-MAPK signalling, which plays a pivotal role in the cell proliferation of multiple human cancers.

No MeSH data available.


Related in: MedlinePlus

Induction of cell proliferation by TERE1 siRNA transfection(A) Human L02 cells transfected with TERE1 siRNA were cultured and quantified by MTT assay. *P<0.05 compared with NC, #P<0.05 compared with NC. Values are means±SD of three separate experiments. (B) Count of cell numbers over 3 days following the transfection with two different TERE1-siRNA oligos (291, 322). Cell numbers are means±SD of three separate experiments.
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Figure 3: Induction of cell proliferation by TERE1 siRNA transfection(A) Human L02 cells transfected with TERE1 siRNA were cultured and quantified by MTT assay. *P<0.05 compared with NC, #P<0.05 compared with NC. Values are means±SD of three separate experiments. (B) Count of cell numbers over 3 days following the transfection with two different TERE1-siRNA oligos (291, 322). Cell numbers are means±SD of three separate experiments.

Mentions: The effect of TERE1 down-regulation on cell proliferation was further measured in TERE1-291, 322-siRNA transfected cells using MTT assay (Figure 3). Addition of TERE1-291, 322-siRNA into L02 cells caused a dramatic increase in the number of viable cells (Figure 3A). Trypan Blue exclusion tests were performed to count the number of cells following the transfection over a 3-day period (Figure 3B). Cells transfected with TERE1 siRNA 291 and 322 experienced dramatic cell proliferation. Knocking down the TERE1 expression resulted in cell proliferation.


Down-regulation of TERE1/UBIAD1 activated Ras-MAPK signalling and induced cell proliferation.

Xia Y, Wei X, Wu S, Wang B, Wang X, Hong L - Cell Biol Int Rep (2010) (2010)

Induction of cell proliferation by TERE1 siRNA transfection(A) Human L02 cells transfected with TERE1 siRNA were cultured and quantified by MTT assay. *P<0.05 compared with NC, #P<0.05 compared with NC. Values are means±SD of three separate experiments. (B) Count of cell numbers over 3 days following the transfection with two different TERE1-siRNA oligos (291, 322). Cell numbers are means±SD of three separate experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475436&req=5

Figure 3: Induction of cell proliferation by TERE1 siRNA transfection(A) Human L02 cells transfected with TERE1 siRNA were cultured and quantified by MTT assay. *P<0.05 compared with NC, #P<0.05 compared with NC. Values are means±SD of three separate experiments. (B) Count of cell numbers over 3 days following the transfection with two different TERE1-siRNA oligos (291, 322). Cell numbers are means±SD of three separate experiments.
Mentions: The effect of TERE1 down-regulation on cell proliferation was further measured in TERE1-291, 322-siRNA transfected cells using MTT assay (Figure 3). Addition of TERE1-291, 322-siRNA into L02 cells caused a dramatic increase in the number of viable cells (Figure 3A). Trypan Blue exclusion tests were performed to count the number of cells following the transfection over a 3-day period (Figure 3B). Cells transfected with TERE1 siRNA 291 and 322 experienced dramatic cell proliferation. Knocking down the TERE1 expression resulted in cell proliferation.

Bottom Line: It was found that decreased TERE1/UBIAD1 expression is closely related to both an increased hTERT expression and activation of Ras-MAPK signalling.When the levels of hTERT expression and ERK phosphorylation were measured, it was found that both of them increased in the above transfected cells, suggesting the activation of Ras-MAPK signalling.Our result is the initial demonstration that down-regulation of TERE1 activates Ras-MAPK signalling and induces subsequent cell proliferation.

View Article: PubMed Central - PubMed

Affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Peoples Republic of China ; †Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Peoples Republic of China.

ABSTRACT
TERE1/UBIAD1 is involved in SCCD (Schnyder crystalline corneal dystrophy) and multiple human cancers. So far, the molecular mechanism of TERE1/UBIAD1 in tumourigenesis is unclear. Here, the expression levels of hTERT and TERE1/UBIAD1 in pathologically proven Chinese TCC (transitional cell carcinoma) samples were measured. It was found that decreased TERE1/UBIAD1 expression is closely related to both an increased hTERT expression and activation of Ras-MAPK signalling. Chemically modified TERE1 siRNA oligos were used to knock down TERE1 expression in human L02 cells. Cells transfected with TERE1 siRNA oligos underwent significant cell proliferation. When the levels of hTERT expression and ERK phosphorylation were measured, it was found that both of them increased in the above transfected cells, suggesting the activation of Ras-MAPK signalling. Addition of the MEK inhibitor U0126 into the transfected L02 cells described above inhibited ERK phosphorylation and hTERT expression. Our result is the initial demonstration that down-regulation of TERE1 activates Ras-MAPK signalling and induces subsequent cell proliferation. TERE1 might be a new negative regulator of Ras-MAPK signalling, which plays a pivotal role in the cell proliferation of multiple human cancers.

No MeSH data available.


Related in: MedlinePlus