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Reversible conversion of epithelial and mesenchymal phenotypes in SV40 large T antigen-immortalized rat liver cell lines.

Takenouchi T, Yoshioka M, Yamanaka N, Kitani H - Cell Biol Int Rep (2010) (2010)

Bottom Line: This process is also essential for liver morphogenesis in embryonic development.To study the cellular and molecular basis of EMT, we established two phenotypically different SV40 large T antigen-immortalized cell lines from rat hepatocytes.The first cell line, which had an epithelial morphology and was established in DMEM (Dulbecco's modified Eagle's medium)/Ham's F-12 (DF)-based medium (RL/DF cells), expressed CK18 (cytokeratin 18), a marker of parenchymal hepatocytes.

View Article: PubMed Central - PubMed

Affiliation: Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Ohwashi 12, Tsukuba, Ibaraki 3058634, Japan.

ABSTRACT
EMT (epithelial-mesenchymal transition) is a key process in the development of liver fibrosis. This process is also essential for liver morphogenesis in embryonic development. To study the cellular and molecular basis of EMT, we established two phenotypically different SV40 large T antigen-immortalized cell lines from rat hepatocytes. The first cell line, which had an epithelial morphology and was established in DMEM (Dulbecco's modified Eagle's medium)/Ham's F-12 (DF)-based medium (RL/DF cells), expressed CK18 (cytokeratin 18), a marker of parenchymal hepatocytes. The other, a mesenchymal-like cell line established in DMEM-based medium (RL/DMEM cells), expressed αSMA (α-smooth muscle actin), a marker of hepatic myofibroblasts. Epithelial RL/DF cells underwent phenotypic changes, such as increased expression of αSMA, when the culture medium was switched to DMEM-based medium. In contrast, mesenchymal RL/DMEM cells were induced to express the epithelial marker CK18 with a concomitant decrease in αSMA expression when the culture medium was replaced with DF-based medium. These cell lines may provide novel in vitro models for the study of the conversion between epithelial and mesenchymal phenotypes during EMT in liver fibrosis and morphogenesis.

No MeSH data available.


Related in: MedlinePlus

Possible origins of SV40LT-immortalized rat liver cellsPhenotypic conversion of the cells can be induced by switching the type of culture medium in a reversible manner.
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Figure 6: Possible origins of SV40LT-immortalized rat liver cellsPhenotypic conversion of the cells can be induced by switching the type of culture medium in a reversible manner.

Mentions: In conclusion, we have demonstrated in the present study that two different SV40LT-immortalized rat liver cell lines, RL/DMEM and RL/DF, have the capability to undergo reversible conversions between mesenchymal and epithelial phenotypes, which can be induced by switching the type of culture medium used (Figure 6). Therefore the present experimental systems using these immortalized rat liver cell lines might be useful for the study of the cellular and molecular mechanisms of the phenotypic conversion of hepatocytes during differentiation and disease conditions. These models also show promise for the investigation of the mechanisms of the early stages of EMT in the liver and the development of therapeutic strategies for liver fibrosis and the control of hepatic carcinoma, which are closely associated with EMT in the liver.


Reversible conversion of epithelial and mesenchymal phenotypes in SV40 large T antigen-immortalized rat liver cell lines.

Takenouchi T, Yoshioka M, Yamanaka N, Kitani H - Cell Biol Int Rep (2010) (2010)

Possible origins of SV40LT-immortalized rat liver cellsPhenotypic conversion of the cells can be induced by switching the type of culture medium in a reversible manner.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475435&req=5

Figure 6: Possible origins of SV40LT-immortalized rat liver cellsPhenotypic conversion of the cells can be induced by switching the type of culture medium in a reversible manner.
Mentions: In conclusion, we have demonstrated in the present study that two different SV40LT-immortalized rat liver cell lines, RL/DMEM and RL/DF, have the capability to undergo reversible conversions between mesenchymal and epithelial phenotypes, which can be induced by switching the type of culture medium used (Figure 6). Therefore the present experimental systems using these immortalized rat liver cell lines might be useful for the study of the cellular and molecular mechanisms of the phenotypic conversion of hepatocytes during differentiation and disease conditions. These models also show promise for the investigation of the mechanisms of the early stages of EMT in the liver and the development of therapeutic strategies for liver fibrosis and the control of hepatic carcinoma, which are closely associated with EMT in the liver.

Bottom Line: This process is also essential for liver morphogenesis in embryonic development.To study the cellular and molecular basis of EMT, we established two phenotypically different SV40 large T antigen-immortalized cell lines from rat hepatocytes.The first cell line, which had an epithelial morphology and was established in DMEM (Dulbecco's modified Eagle's medium)/Ham's F-12 (DF)-based medium (RL/DF cells), expressed CK18 (cytokeratin 18), a marker of parenchymal hepatocytes.

View Article: PubMed Central - PubMed

Affiliation: Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Ohwashi 12, Tsukuba, Ibaraki 3058634, Japan.

ABSTRACT
EMT (epithelial-mesenchymal transition) is a key process in the development of liver fibrosis. This process is also essential for liver morphogenesis in embryonic development. To study the cellular and molecular basis of EMT, we established two phenotypically different SV40 large T antigen-immortalized cell lines from rat hepatocytes. The first cell line, which had an epithelial morphology and was established in DMEM (Dulbecco's modified Eagle's medium)/Ham's F-12 (DF)-based medium (RL/DF cells), expressed CK18 (cytokeratin 18), a marker of parenchymal hepatocytes. The other, a mesenchymal-like cell line established in DMEM-based medium (RL/DMEM cells), expressed αSMA (α-smooth muscle actin), a marker of hepatic myofibroblasts. Epithelial RL/DF cells underwent phenotypic changes, such as increased expression of αSMA, when the culture medium was switched to DMEM-based medium. In contrast, mesenchymal RL/DMEM cells were induced to express the epithelial marker CK18 with a concomitant decrease in αSMA expression when the culture medium was replaced with DF-based medium. These cell lines may provide novel in vitro models for the study of the conversion between epithelial and mesenchymal phenotypes during EMT in liver fibrosis and morphogenesis.

No MeSH data available.


Related in: MedlinePlus