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In vivo induction of apoptosis by fucoxanthin, a marine carotenoid, associated with down-regulating STAT3/EGFR signaling in sarcoma 180 (S180) xenografts-bearing mice.

Wang J, Chen S, Xu S, Yu X, Ma D, Hu X, Cao X - Mar Drugs (2012)

Bottom Line: But the precise mechanism by which fucoxanthin exerts anticarcinogenic effects is not yet fully understood.In addition, immunohistochemistry analysis and Western blotting analysis showed that fucoxanthin significantly decreased the expressions of survivin and vascular endothelial growth factor (VEGF).Most importantly, fucoxanthin inhibited the expressions of the epidermal growth factor receptor (EGFR) and STAT3 and phosphorylated STAT3 proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, College of Medicine, Wuhan University of Science and Technology, Wuhan 430065, China. wangj79@hotmail.com

ABSTRACT
Previous in vitro researches have showed that fucoxanthin, a natural carotenoid isolated from sargassum, can inhibit proliferation or induce apoptosis in human neuroblastoma, hepatoma, leukemia, colon carcinoma, prostate cancer or urinary bladder cancer cells. But the precise mechanism by which fucoxanthin exerts anticarcinogenic effects is not yet fully understood. In this study, we performed an in vivo study to investigate the anti-tumor effect and mechanisms of fucoxanthin on xenografted sarcoma 180 (S180) in mice. Results revealed that fucoxanthin significantly inhibited the growth of sarcoma at the dose of 50 or 100 mg/kg. TUNEL analysis showed that the number of positive cells in the fucoxanthin-treated group was higher than that in the control group. Western blotting analysis also revealed the suppressed expression of bcl-2 and enhanced expression of cleaved caspase-3 by fucoxanthin. In addition, immunohistochemistry analysis and Western blotting analysis showed that fucoxanthin significantly decreased the expressions of survivin and vascular endothelial growth factor (VEGF). Most importantly, fucoxanthin inhibited the expressions of the epidermal growth factor receptor (EGFR) and STAT3 and phosphorylated STAT3 proteins. These results indicated that in vivo induction of apoptosis by fucoxanthin is associated with down-regulating STAT3/EGFR signaling in S180 xenografts-bearing mice.

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Expression of survivin protein in sarcoma tissue. Western blotting analysis was used to detect the effects of fucoxanthin on the expression of survivin in S180 tumor tissue. The expression of survivin was dramatically decreased by fucoxanthin at the dose of 50 and 100 mg/kg. Values are the mean ± SD. **p < 0.01 vs. control group.
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marinedrugs-10-02055-f003: Expression of survivin protein in sarcoma tissue. Western blotting analysis was used to detect the effects of fucoxanthin on the expression of survivin in S180 tumor tissue. The expression of survivin was dramatically decreased by fucoxanthin at the dose of 50 and 100 mg/kg. Values are the mean ± SD. **p < 0.01 vs. control group.

Mentions: To explore the anti-tumor mechanisms of fucoxanthin in S180 tumor, we examined the protein expressions of survivin and VEGF. Western blotting analysis was used to detect the effects of fucoxanthin on the expression of survivin in S180 tumor tissue. The result showed the expression of survivin was dramatically decreased by CTX and fucoxanthin at the dose of 50 or 100 mg/kg (p < 0.05 or p < 0.01, vs. model control group, Figure 3). Immunohistochemistry analysis showed that the expression of VEGF-positive cells were obviously down-regulated by CTX and fucoxanthin (Figure 4A). Western blotting analysis also confirmed that the expression of VEGF (Figure 4B) was decreased by CTX and fucoxanthin at the dose of 50 or 100 mg/kg compared with the control group (p < 0.05 or p < 0.01).


In vivo induction of apoptosis by fucoxanthin, a marine carotenoid, associated with down-regulating STAT3/EGFR signaling in sarcoma 180 (S180) xenografts-bearing mice.

Wang J, Chen S, Xu S, Yu X, Ma D, Hu X, Cao X - Mar Drugs (2012)

Expression of survivin protein in sarcoma tissue. Western blotting analysis was used to detect the effects of fucoxanthin on the expression of survivin in S180 tumor tissue. The expression of survivin was dramatically decreased by fucoxanthin at the dose of 50 and 100 mg/kg. Values are the mean ± SD. **p < 0.01 vs. control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475273&req=5

marinedrugs-10-02055-f003: Expression of survivin protein in sarcoma tissue. Western blotting analysis was used to detect the effects of fucoxanthin on the expression of survivin in S180 tumor tissue. The expression of survivin was dramatically decreased by fucoxanthin at the dose of 50 and 100 mg/kg. Values are the mean ± SD. **p < 0.01 vs. control group.
Mentions: To explore the anti-tumor mechanisms of fucoxanthin in S180 tumor, we examined the protein expressions of survivin and VEGF. Western blotting analysis was used to detect the effects of fucoxanthin on the expression of survivin in S180 tumor tissue. The result showed the expression of survivin was dramatically decreased by CTX and fucoxanthin at the dose of 50 or 100 mg/kg (p < 0.05 or p < 0.01, vs. model control group, Figure 3). Immunohistochemistry analysis showed that the expression of VEGF-positive cells were obviously down-regulated by CTX and fucoxanthin (Figure 4A). Western blotting analysis also confirmed that the expression of VEGF (Figure 4B) was decreased by CTX and fucoxanthin at the dose of 50 or 100 mg/kg compared with the control group (p < 0.05 or p < 0.01).

Bottom Line: But the precise mechanism by which fucoxanthin exerts anticarcinogenic effects is not yet fully understood.In addition, immunohistochemistry analysis and Western blotting analysis showed that fucoxanthin significantly decreased the expressions of survivin and vascular endothelial growth factor (VEGF).Most importantly, fucoxanthin inhibited the expressions of the epidermal growth factor receptor (EGFR) and STAT3 and phosphorylated STAT3 proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, College of Medicine, Wuhan University of Science and Technology, Wuhan 430065, China. wangj79@hotmail.com

ABSTRACT
Previous in vitro researches have showed that fucoxanthin, a natural carotenoid isolated from sargassum, can inhibit proliferation or induce apoptosis in human neuroblastoma, hepatoma, leukemia, colon carcinoma, prostate cancer or urinary bladder cancer cells. But the precise mechanism by which fucoxanthin exerts anticarcinogenic effects is not yet fully understood. In this study, we performed an in vivo study to investigate the anti-tumor effect and mechanisms of fucoxanthin on xenografted sarcoma 180 (S180) in mice. Results revealed that fucoxanthin significantly inhibited the growth of sarcoma at the dose of 50 or 100 mg/kg. TUNEL analysis showed that the number of positive cells in the fucoxanthin-treated group was higher than that in the control group. Western blotting analysis also revealed the suppressed expression of bcl-2 and enhanced expression of cleaved caspase-3 by fucoxanthin. In addition, immunohistochemistry analysis and Western blotting analysis showed that fucoxanthin significantly decreased the expressions of survivin and vascular endothelial growth factor (VEGF). Most importantly, fucoxanthin inhibited the expressions of the epidermal growth factor receptor (EGFR) and STAT3 and phosphorylated STAT3 proteins. These results indicated that in vivo induction of apoptosis by fucoxanthin is associated with down-regulating STAT3/EGFR signaling in S180 xenografts-bearing mice.

Show MeSH
Related in: MedlinePlus