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In vivo induction of apoptosis by fucoxanthin, a marine carotenoid, associated with down-regulating STAT3/EGFR signaling in sarcoma 180 (S180) xenografts-bearing mice.

Wang J, Chen S, Xu S, Yu X, Ma D, Hu X, Cao X - Mar Drugs (2012)

Bottom Line: But the precise mechanism by which fucoxanthin exerts anticarcinogenic effects is not yet fully understood.In addition, immunohistochemistry analysis and Western blotting analysis showed that fucoxanthin significantly decreased the expressions of survivin and vascular endothelial growth factor (VEGF).Most importantly, fucoxanthin inhibited the expressions of the epidermal growth factor receptor (EGFR) and STAT3 and phosphorylated STAT3 proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, College of Medicine, Wuhan University of Science and Technology, Wuhan 430065, China. wangj79@hotmail.com

ABSTRACT
Previous in vitro researches have showed that fucoxanthin, a natural carotenoid isolated from sargassum, can inhibit proliferation or induce apoptosis in human neuroblastoma, hepatoma, leukemia, colon carcinoma, prostate cancer or urinary bladder cancer cells. But the precise mechanism by which fucoxanthin exerts anticarcinogenic effects is not yet fully understood. In this study, we performed an in vivo study to investigate the anti-tumor effect and mechanisms of fucoxanthin on xenografted sarcoma 180 (S180) in mice. Results revealed that fucoxanthin significantly inhibited the growth of sarcoma at the dose of 50 or 100 mg/kg. TUNEL analysis showed that the number of positive cells in the fucoxanthin-treated group was higher than that in the control group. Western blotting analysis also revealed the suppressed expression of bcl-2 and enhanced expression of cleaved caspase-3 by fucoxanthin. In addition, immunohistochemistry analysis and Western blotting analysis showed that fucoxanthin significantly decreased the expressions of survivin and vascular endothelial growth factor (VEGF). Most importantly, fucoxanthin inhibited the expressions of the epidermal growth factor receptor (EGFR) and STAT3 and phosphorylated STAT3 proteins. These results indicated that in vivo induction of apoptosis by fucoxanthin is associated with down-regulating STAT3/EGFR signaling in S180 xenografts-bearing mice.

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The protein expressions of bcl-2 and cleaved caspase-3 in sarcoma tissue (A,B). Tissue homogenates from S180 sarcomas were prepared, and then the expression levels of bcl-2 (A) and cleaved caspase-3 (B) proteins in sarcoma were detected by western blotting. Values are the mean ± SD.
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marinedrugs-10-02055-f002: The protein expressions of bcl-2 and cleaved caspase-3 in sarcoma tissue (A,B). Tissue homogenates from S180 sarcomas were prepared, and then the expression levels of bcl-2 (A) and cleaved caspase-3 (B) proteins in sarcoma were detected by western blotting. Values are the mean ± SD.

Mentions: We further investigated the expression levels of two key apoptosis-related genes—caspase-3 and bcl-2 in tumor tissue, which were determined by western blotting (Figure 2A,B). The pro-apoptotic cleaved caspase-3 protein level was obviously increased, while the anti-apoptosis gene bcl-2 protein level was decreased by CTX (p < 0.01, vs. model control group), fucoxanthin at the dose of 50 and 100 mg/kg (p < 0.05 or p < 0.01, vs. model control group).


In vivo induction of apoptosis by fucoxanthin, a marine carotenoid, associated with down-regulating STAT3/EGFR signaling in sarcoma 180 (S180) xenografts-bearing mice.

Wang J, Chen S, Xu S, Yu X, Ma D, Hu X, Cao X - Mar Drugs (2012)

The protein expressions of bcl-2 and cleaved caspase-3 in sarcoma tissue (A,B). Tissue homogenates from S180 sarcomas were prepared, and then the expression levels of bcl-2 (A) and cleaved caspase-3 (B) proteins in sarcoma were detected by western blotting. Values are the mean ± SD.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475273&req=5

marinedrugs-10-02055-f002: The protein expressions of bcl-2 and cleaved caspase-3 in sarcoma tissue (A,B). Tissue homogenates from S180 sarcomas were prepared, and then the expression levels of bcl-2 (A) and cleaved caspase-3 (B) proteins in sarcoma were detected by western blotting. Values are the mean ± SD.
Mentions: We further investigated the expression levels of two key apoptosis-related genes—caspase-3 and bcl-2 in tumor tissue, which were determined by western blotting (Figure 2A,B). The pro-apoptotic cleaved caspase-3 protein level was obviously increased, while the anti-apoptosis gene bcl-2 protein level was decreased by CTX (p < 0.01, vs. model control group), fucoxanthin at the dose of 50 and 100 mg/kg (p < 0.05 or p < 0.01, vs. model control group).

Bottom Line: But the precise mechanism by which fucoxanthin exerts anticarcinogenic effects is not yet fully understood.In addition, immunohistochemistry analysis and Western blotting analysis showed that fucoxanthin significantly decreased the expressions of survivin and vascular endothelial growth factor (VEGF).Most importantly, fucoxanthin inhibited the expressions of the epidermal growth factor receptor (EGFR) and STAT3 and phosphorylated STAT3 proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, College of Medicine, Wuhan University of Science and Technology, Wuhan 430065, China. wangj79@hotmail.com

ABSTRACT
Previous in vitro researches have showed that fucoxanthin, a natural carotenoid isolated from sargassum, can inhibit proliferation or induce apoptosis in human neuroblastoma, hepatoma, leukemia, colon carcinoma, prostate cancer or urinary bladder cancer cells. But the precise mechanism by which fucoxanthin exerts anticarcinogenic effects is not yet fully understood. In this study, we performed an in vivo study to investigate the anti-tumor effect and mechanisms of fucoxanthin on xenografted sarcoma 180 (S180) in mice. Results revealed that fucoxanthin significantly inhibited the growth of sarcoma at the dose of 50 or 100 mg/kg. TUNEL analysis showed that the number of positive cells in the fucoxanthin-treated group was higher than that in the control group. Western blotting analysis also revealed the suppressed expression of bcl-2 and enhanced expression of cleaved caspase-3 by fucoxanthin. In addition, immunohistochemistry analysis and Western blotting analysis showed that fucoxanthin significantly decreased the expressions of survivin and vascular endothelial growth factor (VEGF). Most importantly, fucoxanthin inhibited the expressions of the epidermal growth factor receptor (EGFR) and STAT3 and phosphorylated STAT3 proteins. These results indicated that in vivo induction of apoptosis by fucoxanthin is associated with down-regulating STAT3/EGFR signaling in S180 xenografts-bearing mice.

Show MeSH
Related in: MedlinePlus