Limits...
Antiproliferative activity of fucan nanogel.

Dantas-Santos N, Almeida-Lima J, Vidal AA, Gomes DL, Oliveira RM, Santos Pedrosa S, Pereira P, Gama FM, Oliveira Rocha HA - Mar Drugs (2012)

Bottom Line: The resulting modified material (SNFuc) formed nanosized particles.On the other hand, nanogel improved Chinese hamster ovary (CHO) and monocyte macrophage cell (RAW) non-tumor cell line proliferation in the same concentration range.The antiproliferative effect against tumor cells was also confirmed using the BrdU test.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN 59078-970, Brazil. nednaldod@hotmail.com

ABSTRACT
Sulfated fucans comprise families of polydisperse natural polysaccharides based on sulfated L-fucose. Our aim was to investigate whether fucan nanogel induces cell-specific responses. To that end, a non toxic fucan extracted from Spatoglossum schröederi was chemically modified by grafting hexadecylamine to the polymer hydrophilic backbone. The resulting modified material (SNFuc) formed nanosized particles. The degree of substitution with hydrophobic chains was close to 100%, as estimated by elemental analysis. SNFfuc in aqueous media had a mean diameter of 123 nm and zeta potential of -38.3 ± 0.74 mV, as measured by dynamic light scattering. Nanoparticles conserved their size for up to 70 days. SNFuc cytotoxicity was determined using the MTT assay after culturing different cell lines for 24 h. Tumor-cell (HepG2, 786, H-S5) proliferation was inhibited by 2.0%-43.7% at nanogel concentrations of 0.05-0.5 mg/mL and rabbit aorta endothelial cells (RAEC) non-tumor cell line proliferation displayed inhibition of 8.0%-22.0%. On the other hand, nanogel improved Chinese hamster ovary (CHO) and monocyte macrophage cell (RAW) non-tumor cell line proliferation in the same concentration range. The antiproliferative effect against tumor cells was also confirmed using the BrdU test. Flow cytometric analysis revealed that the fucan nanogel inhibited 786 cell proliferation through caspase and caspase-independent mechanisms. In addition, SNFuc blocks 786 cell passages in the S and G2-M phases of the cell cycle.

Show MeSH

Related in: MedlinePlus

Changes in the percentage of normal, apoptotic and necrotic cells after SNFuc-TBA nanogel incubation. (A) Control; (B) Cells maintainedin the presence of0.5mg/mLSNFuc-TBA; (C) Cells kept in the presence of0.5mg/mLSNFuc and ZVAD-FMK; (D) Cells maintainedin the presence of0.5mg/mLSNFuc and E-64. After 24 h, cells were treated withAnnexinV/PI and analyzed by Flow Cytometry. Q1: AnnexinVnegative/PIpositive;Q2:AnnexinV/PIpositive;Q3:AnnexinVpositive/PI negative;Q4:AnnexinV/PInegative. Analysis flow cytometry data was performed using FlowJosoftwarev.7.6.3. Similar results were obtained in three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3475269&req=5

marinedrugs-10-02002-f008: Changes in the percentage of normal, apoptotic and necrotic cells after SNFuc-TBA nanogel incubation. (A) Control; (B) Cells maintainedin the presence of0.5mg/mLSNFuc-TBA; (C) Cells kept in the presence of0.5mg/mLSNFuc and ZVAD-FMK; (D) Cells maintainedin the presence of0.5mg/mLSNFuc and E-64. After 24 h, cells were treated withAnnexinV/PI and analyzed by Flow Cytometry. Q1: AnnexinVnegative/PIpositive;Q2:AnnexinV/PIpositive;Q3:AnnexinVpositive/PI negative;Q4:AnnexinV/PInegative. Analysis flow cytometry data was performed using FlowJosoftwarev.7.6.3. Similar results were obtained in three independent experiments.

Mentions: In order to observe additional influences of SNFuc nanogel on 786 cell lines, apoptotic and necrotic status were analyzed. SNFuc (0.5 mg/mL) significantly (p < 0.001) increased the percentage of cells in early (from 2.5 to 14.0%) and late apoptosis (from 5.5 to 21.5%), whereas the percentage of necrotic cells was not significantly affected (Figure 8B).


Antiproliferative activity of fucan nanogel.

Dantas-Santos N, Almeida-Lima J, Vidal AA, Gomes DL, Oliveira RM, Santos Pedrosa S, Pereira P, Gama FM, Oliveira Rocha HA - Mar Drugs (2012)

Changes in the percentage of normal, apoptotic and necrotic cells after SNFuc-TBA nanogel incubation. (A) Control; (B) Cells maintainedin the presence of0.5mg/mLSNFuc-TBA; (C) Cells kept in the presence of0.5mg/mLSNFuc and ZVAD-FMK; (D) Cells maintainedin the presence of0.5mg/mLSNFuc and E-64. After 24 h, cells were treated withAnnexinV/PI and analyzed by Flow Cytometry. Q1: AnnexinVnegative/PIpositive;Q2:AnnexinV/PIpositive;Q3:AnnexinVpositive/PI negative;Q4:AnnexinV/PInegative. Analysis flow cytometry data was performed using FlowJosoftwarev.7.6.3. Similar results were obtained in three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475269&req=5

marinedrugs-10-02002-f008: Changes in the percentage of normal, apoptotic and necrotic cells after SNFuc-TBA nanogel incubation. (A) Control; (B) Cells maintainedin the presence of0.5mg/mLSNFuc-TBA; (C) Cells kept in the presence of0.5mg/mLSNFuc and ZVAD-FMK; (D) Cells maintainedin the presence of0.5mg/mLSNFuc and E-64. After 24 h, cells were treated withAnnexinV/PI and analyzed by Flow Cytometry. Q1: AnnexinVnegative/PIpositive;Q2:AnnexinV/PIpositive;Q3:AnnexinVpositive/PI negative;Q4:AnnexinV/PInegative. Analysis flow cytometry data was performed using FlowJosoftwarev.7.6.3. Similar results were obtained in three independent experiments.
Mentions: In order to observe additional influences of SNFuc nanogel on 786 cell lines, apoptotic and necrotic status were analyzed. SNFuc (0.5 mg/mL) significantly (p < 0.001) increased the percentage of cells in early (from 2.5 to 14.0%) and late apoptosis (from 5.5 to 21.5%), whereas the percentage of necrotic cells was not significantly affected (Figure 8B).

Bottom Line: The resulting modified material (SNFuc) formed nanosized particles.On the other hand, nanogel improved Chinese hamster ovary (CHO) and monocyte macrophage cell (RAW) non-tumor cell line proliferation in the same concentration range.The antiproliferative effect against tumor cells was also confirmed using the BrdU test.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN 59078-970, Brazil. nednaldod@hotmail.com

ABSTRACT
Sulfated fucans comprise families of polydisperse natural polysaccharides based on sulfated L-fucose. Our aim was to investigate whether fucan nanogel induces cell-specific responses. To that end, a non toxic fucan extracted from Spatoglossum schröederi was chemically modified by grafting hexadecylamine to the polymer hydrophilic backbone. The resulting modified material (SNFuc) formed nanosized particles. The degree of substitution with hydrophobic chains was close to 100%, as estimated by elemental analysis. SNFfuc in aqueous media had a mean diameter of 123 nm and zeta potential of -38.3 ± 0.74 mV, as measured by dynamic light scattering. Nanoparticles conserved their size for up to 70 days. SNFuc cytotoxicity was determined using the MTT assay after culturing different cell lines for 24 h. Tumor-cell (HepG2, 786, H-S5) proliferation was inhibited by 2.0%-43.7% at nanogel concentrations of 0.05-0.5 mg/mL and rabbit aorta endothelial cells (RAEC) non-tumor cell line proliferation displayed inhibition of 8.0%-22.0%. On the other hand, nanogel improved Chinese hamster ovary (CHO) and monocyte macrophage cell (RAW) non-tumor cell line proliferation in the same concentration range. The antiproliferative effect against tumor cells was also confirmed using the BrdU test. Flow cytometric analysis revealed that the fucan nanogel inhibited 786 cell proliferation through caspase and caspase-independent mechanisms. In addition, SNFuc blocks 786 cell passages in the S and G2-M phases of the cell cycle.

Show MeSH
Related in: MedlinePlus