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Antiproliferative activity of fucan nanogel.

Dantas-Santos N, Almeida-Lima J, Vidal AA, Gomes DL, Oliveira RM, Santos Pedrosa S, Pereira P, Gama FM, Oliveira Rocha HA - Mar Drugs (2012)

Bottom Line: The resulting modified material (SNFuc) formed nanosized particles.On the other hand, nanogel improved Chinese hamster ovary (CHO) and monocyte macrophage cell (RAW) non-tumor cell line proliferation in the same concentration range.The antiproliferative effect against tumor cells was also confirmed using the BrdU test.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN 59078-970, Brazil. nednaldod@hotmail.com

ABSTRACT
Sulfated fucans comprise families of polydisperse natural polysaccharides based on sulfated L-fucose. Our aim was to investigate whether fucan nanogel induces cell-specific responses. To that end, a non toxic fucan extracted from Spatoglossum schröederi was chemically modified by grafting hexadecylamine to the polymer hydrophilic backbone. The resulting modified material (SNFuc) formed nanosized particles. The degree of substitution with hydrophobic chains was close to 100%, as estimated by elemental analysis. SNFfuc in aqueous media had a mean diameter of 123 nm and zeta potential of -38.3 ± 0.74 mV, as measured by dynamic light scattering. Nanoparticles conserved their size for up to 70 days. SNFuc cytotoxicity was determined using the MTT assay after culturing different cell lines for 24 h. Tumor-cell (HepG2, 786, H-S5) proliferation was inhibited by 2.0%-43.7% at nanogel concentrations of 0.05-0.5 mg/mL and rabbit aorta endothelial cells (RAEC) non-tumor cell line proliferation displayed inhibition of 8.0%-22.0%. On the other hand, nanogel improved Chinese hamster ovary (CHO) and monocyte macrophage cell (RAW) non-tumor cell line proliferation in the same concentration range. The antiproliferative effect against tumor cells was also confirmed using the BrdU test. Flow cytometric analysis revealed that the fucan nanogel inhibited 786 cell proliferation through caspase and caspase-independent mechanisms. In addition, SNFuc blocks 786 cell passages in the S and G2-M phases of the cell cycle.

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FTIR spectra of native fucan and SNFuc.
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marinedrugs-10-02002-f002: FTIR spectra of native fucan and SNFuc.

Mentions: The FI-IR analysis of native fucan and SNFuc is showed in Figure 2. Characteristic sulfate absorptions were identified in the FT-IR spectra of compounds: bands around 1274 cm−1 for asymmetric S=O stretching vibration and bands around 1045 cm−1 for symmetric C–O vibration associated with a C–O–SO3 group. The peaks at 810–850 were caused by the bending vibration of C–O–S [19]. At 3000–3400 cm−1 Fuc A and SNFuc showed bands from the stretching vibration of O–H and C–H, respectively [20]. However, the SNFuc FI-IR spectrum showed the intensity of these bands increased due the presence of N–H (3000–3400 cm−1) and stretching vibrations of CH2 in hexadecyl residues (2921 and around 2850 cm−1) [21]. The peak of the C–H symmetric deformation vibration was at 1427 cm−1 [22]. The intensities of this absorption band increased with chain length of the CH2 groups in SNFuc. A band at 1616 cm−1 was identified only in fucan A spectrum and was assigned to antisymmetric stretching vibration of COO− of glucuronic acid [23]. The presence glucuronic acid was also confirmed with a symmetric vibration peak around 1410 cm−1. On the other hand, SNFuc spectrum showed a band at 1740 cm−1 caused by C=O stretch vibrations in COOH and esters [24]. The band at1643 cm−1 was due the amine I vibration which is overlapped with the vibration of water. Less intense peak around1550 cm−1 arose from amide II vibration in alkylamides and thus confirmed amidation. Additionally, band at 620 cm−1 was assigned to N–C=O bending vibration [24].


Antiproliferative activity of fucan nanogel.

Dantas-Santos N, Almeida-Lima J, Vidal AA, Gomes DL, Oliveira RM, Santos Pedrosa S, Pereira P, Gama FM, Oliveira Rocha HA - Mar Drugs (2012)

FTIR spectra of native fucan and SNFuc.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475269&req=5

marinedrugs-10-02002-f002: FTIR spectra of native fucan and SNFuc.
Mentions: The FI-IR analysis of native fucan and SNFuc is showed in Figure 2. Characteristic sulfate absorptions were identified in the FT-IR spectra of compounds: bands around 1274 cm−1 for asymmetric S=O stretching vibration and bands around 1045 cm−1 for symmetric C–O vibration associated with a C–O–SO3 group. The peaks at 810–850 were caused by the bending vibration of C–O–S [19]. At 3000–3400 cm−1 Fuc A and SNFuc showed bands from the stretching vibration of O–H and C–H, respectively [20]. However, the SNFuc FI-IR spectrum showed the intensity of these bands increased due the presence of N–H (3000–3400 cm−1) and stretching vibrations of CH2 in hexadecyl residues (2921 and around 2850 cm−1) [21]. The peak of the C–H symmetric deformation vibration was at 1427 cm−1 [22]. The intensities of this absorption band increased with chain length of the CH2 groups in SNFuc. A band at 1616 cm−1 was identified only in fucan A spectrum and was assigned to antisymmetric stretching vibration of COO− of glucuronic acid [23]. The presence glucuronic acid was also confirmed with a symmetric vibration peak around 1410 cm−1. On the other hand, SNFuc spectrum showed a band at 1740 cm−1 caused by C=O stretch vibrations in COOH and esters [24]. The band at1643 cm−1 was due the amine I vibration which is overlapped with the vibration of water. Less intense peak around1550 cm−1 arose from amide II vibration in alkylamides and thus confirmed amidation. Additionally, band at 620 cm−1 was assigned to N–C=O bending vibration [24].

Bottom Line: The resulting modified material (SNFuc) formed nanosized particles.On the other hand, nanogel improved Chinese hamster ovary (CHO) and monocyte macrophage cell (RAW) non-tumor cell line proliferation in the same concentration range.The antiproliferative effect against tumor cells was also confirmed using the BrdU test.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN 59078-970, Brazil. nednaldod@hotmail.com

ABSTRACT
Sulfated fucans comprise families of polydisperse natural polysaccharides based on sulfated L-fucose. Our aim was to investigate whether fucan nanogel induces cell-specific responses. To that end, a non toxic fucan extracted from Spatoglossum schröederi was chemically modified by grafting hexadecylamine to the polymer hydrophilic backbone. The resulting modified material (SNFuc) formed nanosized particles. The degree of substitution with hydrophobic chains was close to 100%, as estimated by elemental analysis. SNFfuc in aqueous media had a mean diameter of 123 nm and zeta potential of -38.3 ± 0.74 mV, as measured by dynamic light scattering. Nanoparticles conserved their size for up to 70 days. SNFuc cytotoxicity was determined using the MTT assay after culturing different cell lines for 24 h. Tumor-cell (HepG2, 786, H-S5) proliferation was inhibited by 2.0%-43.7% at nanogel concentrations of 0.05-0.5 mg/mL and rabbit aorta endothelial cells (RAEC) non-tumor cell line proliferation displayed inhibition of 8.0%-22.0%. On the other hand, nanogel improved Chinese hamster ovary (CHO) and monocyte macrophage cell (RAW) non-tumor cell line proliferation in the same concentration range. The antiproliferative effect against tumor cells was also confirmed using the BrdU test. Flow cytometric analysis revealed that the fucan nanogel inhibited 786 cell proliferation through caspase and caspase-independent mechanisms. In addition, SNFuc blocks 786 cell passages in the S and G2-M phases of the cell cycle.

Show MeSH
Related in: MedlinePlus