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Antinociceptive and anti-inflammatory activities of crude methanolic extract of red alga Bryothamnion triquetrum.

Cavalcante-Silva LH, da Matta CB, de Araújo MV, Barbosa-Filho JM, de Lira DP, de Oliveira Santos BV, de Miranda GE, Alexandre-Moreira MS - Mar Drugs (2012)

Bottom Line: While BT-MeOH did not inhibit the neurogenic phase in formalin-induced nociception, the inflammatory phase was inhibited by 53.1% (66.8 ± 14.2 s; p < 0.01).Based on the results obtained in this study, we conclude that BT-MeOH has peripheral antinociceptive and anti-inflammatory activities.However, more studies need to be conducted to confirm these properties.

View Article: PubMed Central - PubMed

Affiliation: LaFI-Laboratory of Pharmacology and Immunity, Institute of Biological Sciences and Health, Federal University of Alagoas, Maceió 57020-720, AL, Brazil. luiz0710@gmail.com

ABSTRACT
The marine environment is an extraordinary reservoir of bioactive natural products, many of which exhibit chemical and structural features not found in terrestrial natural products. In this regard, the aim of this study was to investigate the possible antinociceptive and anti-inflammatory activities of a crude methanolic extract of the red alga Bryothamnion triquetrum (BT-MeOH) in murine models. Groups of Swiss mice of both sexes (25-30 g) were used throughout the experiments. The potential antinociceptive of BT-MeOH was evaluated by means of the following tests: acetic acid-induced writhing, hot-plate test and glutamate- and formalin-induced nociception. The anti-inflammatory activity of BT-MeOH was investigated using the zymosan A-induced peritonitis test. The tests were conducted using 100 mg/kg (p.o.) BT-MeOH, 33.3 mg/kg (p.o.) dipyrone, 35.7 mg/kg (p.o.) indomethacin and 5.7 mg/kg (s.c.) morphine. The extract and all standard drugs were administered 40 min before the nociceptive/inflammatory stimulus. In the acetic acid-induced writhing test, BT-MeOH and dipyrone inhibited the nociceptive response by 55.9% (22.2 ± 2.0 writhings; p < 0.01) and 80.9% (9.6 ± 2.1 writhings; p < 0.01). In the hot-plate test, BT-MeOH did not increase the latency time of the animals in the time evaluated. In addition, BT-MeOH inhibited glutamate-induced nociception by 50.1%. While BT-MeOH did not inhibit the neurogenic phase in formalin-induced nociception, the inflammatory phase was inhibited by 53.1% (66.8 ± 14.2 s; p < 0.01). Indomethacin inhibited the inflammatory phase by 60.2% (56.8 ± 8.7 s; p < 0.01). In the zymosan-induced peritonitis test, BT-MeOH inhibited 55.6% (6.6 ± 0.2 × 10(6) leukocytes/mL; p < 0.01) of leukocyte migration, while indomethacin inhibited 78.1% (3.2 ± 0.1 × 10(6) leukocytes/mL; p < 0.01). Based on the results obtained in this study, we conclude that BT-MeOH has peripheral antinociceptive and anti-inflammatory activities. However, more studies need to be conducted to confirm these properties.

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Antinociceptive effect of the BT-MeOH (100 mg/kg, p.o.) and morphine (5.7 mg/kg, s.c.) in glutamate-induced nociception test. Each column represents the mean ± S.E.M. of six animals. Statistical differences between the treated and control groups were evaluated by ANOVA and Dunnett’s test, and the asterisks denote the level of significance in comparison with control group, *** p < 0.001.
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marinedrugs-10-01977-f004: Antinociceptive effect of the BT-MeOH (100 mg/kg, p.o.) and morphine (5.7 mg/kg, s.c.) in glutamate-induced nociception test. Each column represents the mean ± S.E.M. of six animals. Statistical differences between the treated and control groups were evaluated by ANOVA and Dunnett’s test, and the asterisks denote the level of significance in comparison with control group, *** p < 0.001.

Mentions: In addition, the algal extract was submitted to the glutamate-induced nociception test. BT-MeOH (100 mg/kg, p.o.) decreased the nociceptive response (50.1% inhibition) significantly (Figure 4). Glutamate is an excitatory neurotransmitter involved in nociceptive primary afferent transmission, and its action involves the activation of NMDA and non-NMDA receptors and depends on the activation of the L-arginine-nitric oxide pathway [65]. Besides its action in the development of the nociceptive response, both peripherally and centrally, its role in the maintenance of such process has been demonstrated by some studies [66,67,68]. This result indicates that BT-MeOH modulates the nociceptive action of the glutamatergic pathway.


Antinociceptive and anti-inflammatory activities of crude methanolic extract of red alga Bryothamnion triquetrum.

Cavalcante-Silva LH, da Matta CB, de Araújo MV, Barbosa-Filho JM, de Lira DP, de Oliveira Santos BV, de Miranda GE, Alexandre-Moreira MS - Mar Drugs (2012)

Antinociceptive effect of the BT-MeOH (100 mg/kg, p.o.) and morphine (5.7 mg/kg, s.c.) in glutamate-induced nociception test. Each column represents the mean ± S.E.M. of six animals. Statistical differences between the treated and control groups were evaluated by ANOVA and Dunnett’s test, and the asterisks denote the level of significance in comparison with control group, *** p < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475267&req=5

marinedrugs-10-01977-f004: Antinociceptive effect of the BT-MeOH (100 mg/kg, p.o.) and morphine (5.7 mg/kg, s.c.) in glutamate-induced nociception test. Each column represents the mean ± S.E.M. of six animals. Statistical differences between the treated and control groups were evaluated by ANOVA and Dunnett’s test, and the asterisks denote the level of significance in comparison with control group, *** p < 0.001.
Mentions: In addition, the algal extract was submitted to the glutamate-induced nociception test. BT-MeOH (100 mg/kg, p.o.) decreased the nociceptive response (50.1% inhibition) significantly (Figure 4). Glutamate is an excitatory neurotransmitter involved in nociceptive primary afferent transmission, and its action involves the activation of NMDA and non-NMDA receptors and depends on the activation of the L-arginine-nitric oxide pathway [65]. Besides its action in the development of the nociceptive response, both peripherally and centrally, its role in the maintenance of such process has been demonstrated by some studies [66,67,68]. This result indicates that BT-MeOH modulates the nociceptive action of the glutamatergic pathway.

Bottom Line: While BT-MeOH did not inhibit the neurogenic phase in formalin-induced nociception, the inflammatory phase was inhibited by 53.1% (66.8 ± 14.2 s; p < 0.01).Based on the results obtained in this study, we conclude that BT-MeOH has peripheral antinociceptive and anti-inflammatory activities.However, more studies need to be conducted to confirm these properties.

View Article: PubMed Central - PubMed

Affiliation: LaFI-Laboratory of Pharmacology and Immunity, Institute of Biological Sciences and Health, Federal University of Alagoas, Maceió 57020-720, AL, Brazil. luiz0710@gmail.com

ABSTRACT
The marine environment is an extraordinary reservoir of bioactive natural products, many of which exhibit chemical and structural features not found in terrestrial natural products. In this regard, the aim of this study was to investigate the possible antinociceptive and anti-inflammatory activities of a crude methanolic extract of the red alga Bryothamnion triquetrum (BT-MeOH) in murine models. Groups of Swiss mice of both sexes (25-30 g) were used throughout the experiments. The potential antinociceptive of BT-MeOH was evaluated by means of the following tests: acetic acid-induced writhing, hot-plate test and glutamate- and formalin-induced nociception. The anti-inflammatory activity of BT-MeOH was investigated using the zymosan A-induced peritonitis test. The tests were conducted using 100 mg/kg (p.o.) BT-MeOH, 33.3 mg/kg (p.o.) dipyrone, 35.7 mg/kg (p.o.) indomethacin and 5.7 mg/kg (s.c.) morphine. The extract and all standard drugs were administered 40 min before the nociceptive/inflammatory stimulus. In the acetic acid-induced writhing test, BT-MeOH and dipyrone inhibited the nociceptive response by 55.9% (22.2 ± 2.0 writhings; p < 0.01) and 80.9% (9.6 ± 2.1 writhings; p < 0.01). In the hot-plate test, BT-MeOH did not increase the latency time of the animals in the time evaluated. In addition, BT-MeOH inhibited glutamate-induced nociception by 50.1%. While BT-MeOH did not inhibit the neurogenic phase in formalin-induced nociception, the inflammatory phase was inhibited by 53.1% (66.8 ± 14.2 s; p < 0.01). Indomethacin inhibited the inflammatory phase by 60.2% (56.8 ± 8.7 s; p < 0.01). In the zymosan-induced peritonitis test, BT-MeOH inhibited 55.6% (6.6 ± 0.2 × 10(6) leukocytes/mL; p < 0.01) of leukocyte migration, while indomethacin inhibited 78.1% (3.2 ± 0.1 × 10(6) leukocytes/mL; p < 0.01). Based on the results obtained in this study, we conclude that BT-MeOH has peripheral antinociceptive and anti-inflammatory activities. However, more studies need to be conducted to confirm these properties.

Show MeSH
Related in: MedlinePlus