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Characterization of isoforms of the lectin isolated from the red algae Bryothamnion seaforthii and its pro-healing effect.

do Nascimento-Neto LG, Carneiro RF, da Silva SR, da Silva BR, Vassiliepe Sousa Arruda F, Carneiro VA, do Nascimento KS, Saker-Sampaio S, da Silva VA, Porto AL, Cavada BS, Sampaio AH, Teixeira EH, Nagano CS - Mar Drugs (2012)

Bottom Line: Because of their biotechnological potential, lectins are widely used in biomedical research.The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry.To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively.

View Article: PubMed Central - PubMed

Affiliation: LIBS, Integrated Laboratory of Biomolecules, Faculty of Medicine of Sobral, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil. ziullec@gmail.com

ABSTRACT
Lectins are a structurally heterogeneous group of proteins that have specific binding sites for carbohydrates and glycoconjugates. Because of their biotechnological potential, lectins are widely used in biomedical research. The present study aimed to evaluate the healing potential of the lectin isolated from the marine red alga Bryothamnion seaforthii (BSL). The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry. For healing tests, skin wounds were induced in the dorsal thoracic region of mice. These animals were randomly divided into three groups and subjected to topical treatment for 12 days with BSL, bovine serum albumin and 150 mM NaCl. To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively. The parameters evaluated included the wound area, the proportion of wound closure and the histological diagnosis. The wound closure was more effective with BSL (Postoperative Day 7 and 12) than controls. The luminal epithelium was completely restructured; the presence of collagen in the dermis and the strongly active presence of young skin annexes demonstrate the potential of treatment with BSL compared with controls. Our findings suggest that BSL has pro-healing properties and can be a potential medical process in the treatment of acute wounds.

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Photomicrographs of surgically induced skin wounds in mice treated with a topical administration of BSA and BSL in POD 12 (Stain: Masson’s trichrome). (A) (BSA). Presence of a scab (white star) and an inflammatory exudate beneath the scab (arrow). Note the progression of the luminal epithelium (yellow arrows) below the crust and inflammatory exudate. Dermis with less collagen deposition and the presence of active granulation tissue (black star) in a regression process is observed. A 4× objective was used; (B) (BSA). Details of the previous photo. Observe the granulation tissue characterized by the presence of vessels (black arrows) and young collagen in the extracellular matrix with inflammatory infiltrate. Note the projection of the epithelium beneath the crust (yellow arrows). A 10× objective was used; (C) (BSL). Note also the newly formed epithelium coated with little production of keratin (black arrows). The presence of a fragment of crust on the edge of the injured area (white star), the formation of skin appendages as sebaceous glands (SG), and the remaining vessels of the granulation tissue can be observed (red arrows). Note the active collagen in the reticular dermis (black star). A 4× objective was used; (D) (BSL). Details of the previous photo showing the presence of cutaneous annexes in the area of the newly formed epithelium (black arrow), fibroblasts in the papillary dermis area (blue arrow) and characteristic neoangiogenesis vessels in the granulation tissue (red arrow). A 40× objective was used.
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marinedrugs-10-01936-f009: Photomicrographs of surgically induced skin wounds in mice treated with a topical administration of BSA and BSL in POD 12 (Stain: Masson’s trichrome). (A) (BSA). Presence of a scab (white star) and an inflammatory exudate beneath the scab (arrow). Note the progression of the luminal epithelium (yellow arrows) below the crust and inflammatory exudate. Dermis with less collagen deposition and the presence of active granulation tissue (black star) in a regression process is observed. A 4× objective was used; (B) (BSA). Details of the previous photo. Observe the granulation tissue characterized by the presence of vessels (black arrows) and young collagen in the extracellular matrix with inflammatory infiltrate. Note the projection of the epithelium beneath the crust (yellow arrows). A 10× objective was used; (C) (BSL). Note also the newly formed epithelium coated with little production of keratin (black arrows). The presence of a fragment of crust on the edge of the injured area (white star), the formation of skin appendages as sebaceous glands (SG), and the remaining vessels of the granulation tissue can be observed (red arrows). Note the active collagen in the reticular dermis (black star). A 4× objective was used; (D) (BSL). Details of the previous photo showing the presence of cutaneous annexes in the area of the newly formed epithelium (black arrow), fibroblasts in the papillary dermis area (blue arrow) and characteristic neoangiogenesis vessels in the granulation tissue (red arrow). A 40× objective was used.

Mentions: At POD 12, the BSL-treated animals experienced restructuring of the epithelium covering with little production of keratin, formation of cutaneous immature annexes, and active collagen in the region of the reticular dermis with presence of fibroblastic activity (Figure 9C,D). In the lesions treated with BSA over the same period, an epithelial scab sealing the epithelium opening, a mild inflammatory infiltrate beneath the crust and progression of the epithelial lining were observed (Figure 9A,B).


Characterization of isoforms of the lectin isolated from the red algae Bryothamnion seaforthii and its pro-healing effect.

do Nascimento-Neto LG, Carneiro RF, da Silva SR, da Silva BR, Vassiliepe Sousa Arruda F, Carneiro VA, do Nascimento KS, Saker-Sampaio S, da Silva VA, Porto AL, Cavada BS, Sampaio AH, Teixeira EH, Nagano CS - Mar Drugs (2012)

Photomicrographs of surgically induced skin wounds in mice treated with a topical administration of BSA and BSL in POD 12 (Stain: Masson’s trichrome). (A) (BSA). Presence of a scab (white star) and an inflammatory exudate beneath the scab (arrow). Note the progression of the luminal epithelium (yellow arrows) below the crust and inflammatory exudate. Dermis with less collagen deposition and the presence of active granulation tissue (black star) in a regression process is observed. A 4× objective was used; (B) (BSA). Details of the previous photo. Observe the granulation tissue characterized by the presence of vessels (black arrows) and young collagen in the extracellular matrix with inflammatory infiltrate. Note the projection of the epithelium beneath the crust (yellow arrows). A 10× objective was used; (C) (BSL). Note also the newly formed epithelium coated with little production of keratin (black arrows). The presence of a fragment of crust on the edge of the injured area (white star), the formation of skin appendages as sebaceous glands (SG), and the remaining vessels of the granulation tissue can be observed (red arrows). Note the active collagen in the reticular dermis (black star). A 4× objective was used; (D) (BSL). Details of the previous photo showing the presence of cutaneous annexes in the area of the newly formed epithelium (black arrow), fibroblasts in the papillary dermis area (blue arrow) and characteristic neoangiogenesis vessels in the granulation tissue (red arrow). A 40× objective was used.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475265&req=5

marinedrugs-10-01936-f009: Photomicrographs of surgically induced skin wounds in mice treated with a topical administration of BSA and BSL in POD 12 (Stain: Masson’s trichrome). (A) (BSA). Presence of a scab (white star) and an inflammatory exudate beneath the scab (arrow). Note the progression of the luminal epithelium (yellow arrows) below the crust and inflammatory exudate. Dermis with less collagen deposition and the presence of active granulation tissue (black star) in a regression process is observed. A 4× objective was used; (B) (BSA). Details of the previous photo. Observe the granulation tissue characterized by the presence of vessels (black arrows) and young collagen in the extracellular matrix with inflammatory infiltrate. Note the projection of the epithelium beneath the crust (yellow arrows). A 10× objective was used; (C) (BSL). Note also the newly formed epithelium coated with little production of keratin (black arrows). The presence of a fragment of crust on the edge of the injured area (white star), the formation of skin appendages as sebaceous glands (SG), and the remaining vessels of the granulation tissue can be observed (red arrows). Note the active collagen in the reticular dermis (black star). A 4× objective was used; (D) (BSL). Details of the previous photo showing the presence of cutaneous annexes in the area of the newly formed epithelium (black arrow), fibroblasts in the papillary dermis area (blue arrow) and characteristic neoangiogenesis vessels in the granulation tissue (red arrow). A 40× objective was used.
Mentions: At POD 12, the BSL-treated animals experienced restructuring of the epithelium covering with little production of keratin, formation of cutaneous immature annexes, and active collagen in the region of the reticular dermis with presence of fibroblastic activity (Figure 9C,D). In the lesions treated with BSA over the same period, an epithelial scab sealing the epithelium opening, a mild inflammatory infiltrate beneath the crust and progression of the epithelial lining were observed (Figure 9A,B).

Bottom Line: Because of their biotechnological potential, lectins are widely used in biomedical research.The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry.To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively.

View Article: PubMed Central - PubMed

Affiliation: LIBS, Integrated Laboratory of Biomolecules, Faculty of Medicine of Sobral, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil. ziullec@gmail.com

ABSTRACT
Lectins are a structurally heterogeneous group of proteins that have specific binding sites for carbohydrates and glycoconjugates. Because of their biotechnological potential, lectins are widely used in biomedical research. The present study aimed to evaluate the healing potential of the lectin isolated from the marine red alga Bryothamnion seaforthii (BSL). The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry. For healing tests, skin wounds were induced in the dorsal thoracic region of mice. These animals were randomly divided into three groups and subjected to topical treatment for 12 days with BSL, bovine serum albumin and 150 mM NaCl. To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively. The parameters evaluated included the wound area, the proportion of wound closure and the histological diagnosis. The wound closure was more effective with BSL (Postoperative Day 7 and 12) than controls. The luminal epithelium was completely restructured; the presence of collagen in the dermis and the strongly active presence of young skin annexes demonstrate the potential of treatment with BSL compared with controls. Our findings suggest that BSL has pro-healing properties and can be a potential medical process in the treatment of acute wounds.

Show MeSH
Related in: MedlinePlus