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Characterization of isoforms of the lectin isolated from the red algae Bryothamnion seaforthii and its pro-healing effect.

do Nascimento-Neto LG, Carneiro RF, da Silva SR, da Silva BR, Vassiliepe Sousa Arruda F, Carneiro VA, do Nascimento KS, Saker-Sampaio S, da Silva VA, Porto AL, Cavada BS, Sampaio AH, Teixeira EH, Nagano CS - Mar Drugs (2012)

Bottom Line: Because of their biotechnological potential, lectins are widely used in biomedical research.The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry.To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively.

View Article: PubMed Central - PubMed

Affiliation: LIBS, Integrated Laboratory of Biomolecules, Faculty of Medicine of Sobral, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil. ziullec@gmail.com

ABSTRACT
Lectins are a structurally heterogeneous group of proteins that have specific binding sites for carbohydrates and glycoconjugates. Because of their biotechnological potential, lectins are widely used in biomedical research. The present study aimed to evaluate the healing potential of the lectin isolated from the marine red alga Bryothamnion seaforthii (BSL). The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry. For healing tests, skin wounds were induced in the dorsal thoracic region of mice. These animals were randomly divided into three groups and subjected to topical treatment for 12 days with BSL, bovine serum albumin and 150 mM NaCl. To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively. The parameters evaluated included the wound area, the proportion of wound closure and the histological diagnosis. The wound closure was more effective with BSL (Postoperative Day 7 and 12) than controls. The luminal epithelium was completely restructured; the presence of collagen in the dermis and the strongly active presence of young skin annexes demonstrate the potential of treatment with BSL compared with controls. Our findings suggest that BSL has pro-healing properties and can be a potential medical process in the treatment of acute wounds.

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Photomicrographs of surgically induced skin wounds in mice treated with a topical administration of BSA and BSL in POD 7 (Stain: Masson’s trichrome). (A) (BSA). Note the presence of a scab (star) covering the wound bed and intense inflammatory exudate (delimited by dashed yellow lines). Below the exudate, observe the area of granulation tissue with congested vessels (arrow) and intense fibroblastic proliferation. Immediately beneath the inflammatory exudate, note the bluish band resulting from the synthesis of collagen (red arrows). A 4× objective was used; (B) (BSA). Details of the granulation tissue. Note the fibroblastic proliferation (arrow) and mild inflammatory infiltrate around the vessels (blue arrow). Note the collagen above the range of the granulation tissue (red arrow). Vessels of the granulation tissue are observed (star). A 10× objective was used; (C) (BSL). Overview of the injured area. Note the presence of a crust sealing the epithelial opening (white star). Vessels of the granulation tissue in the reactional adipose tissue (RAT) surrounded by thickening collagen fibers (arrows) resulted from intense fibroblastic synthesis. An area with a mild inflammatory exudate is observed (black star). A 10× objective was used; (D) (BSL). Detail from the previous photo showing the area of granulation tissue vessels surrounded by active collagen. Note the active fibroblasts in the area (arrows). A 40× objective was used.
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marinedrugs-10-01936-f008: Photomicrographs of surgically induced skin wounds in mice treated with a topical administration of BSA and BSL in POD 7 (Stain: Masson’s trichrome). (A) (BSA). Note the presence of a scab (star) covering the wound bed and intense inflammatory exudate (delimited by dashed yellow lines). Below the exudate, observe the area of granulation tissue with congested vessels (arrow) and intense fibroblastic proliferation. Immediately beneath the inflammatory exudate, note the bluish band resulting from the synthesis of collagen (red arrows). A 4× objective was used; (B) (BSA). Details of the granulation tissue. Note the fibroblastic proliferation (arrow) and mild inflammatory infiltrate around the vessels (blue arrow). Note the collagen above the range of the granulation tissue (red arrow). Vessels of the granulation tissue are observed (star). A 10× objective was used; (C) (BSL). Overview of the injured area. Note the presence of a crust sealing the epithelial opening (white star). Vessels of the granulation tissue in the reactional adipose tissue (RAT) surrounded by thickening collagen fibers (arrows) resulted from intense fibroblastic synthesis. An area with a mild inflammatory exudate is observed (black star). A 10× objective was used; (D) (BSL). Detail from the previous photo showing the area of granulation tissue vessels surrounded by active collagen. Note the active fibroblasts in the area (arrows). A 40× objective was used.

Mentions: On POD 7, densification of collagen fibers in the reticular dermis, which is primarily surrounded by the vessels of the granulation tissue in adipose tissue with the presence of discrete reactional inflammatory exudates, was observed (Figure 8C,D). In the group treated with BSA (Figure 8A,B), intense inflammatory exudates and early fibroblastic proliferation with moderate collagen synthesis were still observed, demonstrating a less evolved healing process when compared with the wounds treated with BSL.


Characterization of isoforms of the lectin isolated from the red algae Bryothamnion seaforthii and its pro-healing effect.

do Nascimento-Neto LG, Carneiro RF, da Silva SR, da Silva BR, Vassiliepe Sousa Arruda F, Carneiro VA, do Nascimento KS, Saker-Sampaio S, da Silva VA, Porto AL, Cavada BS, Sampaio AH, Teixeira EH, Nagano CS - Mar Drugs (2012)

Photomicrographs of surgically induced skin wounds in mice treated with a topical administration of BSA and BSL in POD 7 (Stain: Masson’s trichrome). (A) (BSA). Note the presence of a scab (star) covering the wound bed and intense inflammatory exudate (delimited by dashed yellow lines). Below the exudate, observe the area of granulation tissue with congested vessels (arrow) and intense fibroblastic proliferation. Immediately beneath the inflammatory exudate, note the bluish band resulting from the synthesis of collagen (red arrows). A 4× objective was used; (B) (BSA). Details of the granulation tissue. Note the fibroblastic proliferation (arrow) and mild inflammatory infiltrate around the vessels (blue arrow). Note the collagen above the range of the granulation tissue (red arrow). Vessels of the granulation tissue are observed (star). A 10× objective was used; (C) (BSL). Overview of the injured area. Note the presence of a crust sealing the epithelial opening (white star). Vessels of the granulation tissue in the reactional adipose tissue (RAT) surrounded by thickening collagen fibers (arrows) resulted from intense fibroblastic synthesis. An area with a mild inflammatory exudate is observed (black star). A 10× objective was used; (D) (BSL). Detail from the previous photo showing the area of granulation tissue vessels surrounded by active collagen. Note the active fibroblasts in the area (arrows). A 40× objective was used.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC3475265&req=5

marinedrugs-10-01936-f008: Photomicrographs of surgically induced skin wounds in mice treated with a topical administration of BSA and BSL in POD 7 (Stain: Masson’s trichrome). (A) (BSA). Note the presence of a scab (star) covering the wound bed and intense inflammatory exudate (delimited by dashed yellow lines). Below the exudate, observe the area of granulation tissue with congested vessels (arrow) and intense fibroblastic proliferation. Immediately beneath the inflammatory exudate, note the bluish band resulting from the synthesis of collagen (red arrows). A 4× objective was used; (B) (BSA). Details of the granulation tissue. Note the fibroblastic proliferation (arrow) and mild inflammatory infiltrate around the vessels (blue arrow). Note the collagen above the range of the granulation tissue (red arrow). Vessels of the granulation tissue are observed (star). A 10× objective was used; (C) (BSL). Overview of the injured area. Note the presence of a crust sealing the epithelial opening (white star). Vessels of the granulation tissue in the reactional adipose tissue (RAT) surrounded by thickening collagen fibers (arrows) resulted from intense fibroblastic synthesis. An area with a mild inflammatory exudate is observed (black star). A 10× objective was used; (D) (BSL). Detail from the previous photo showing the area of granulation tissue vessels surrounded by active collagen. Note the active fibroblasts in the area (arrows). A 40× objective was used.
Mentions: On POD 7, densification of collagen fibers in the reticular dermis, which is primarily surrounded by the vessels of the granulation tissue in adipose tissue with the presence of discrete reactional inflammatory exudates, was observed (Figure 8C,D). In the group treated with BSA (Figure 8A,B), intense inflammatory exudates and early fibroblastic proliferation with moderate collagen synthesis were still observed, demonstrating a less evolved healing process when compared with the wounds treated with BSL.

Bottom Line: Because of their biotechnological potential, lectins are widely used in biomedical research.The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry.To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively.

View Article: PubMed Central - PubMed

Affiliation: LIBS, Integrated Laboratory of Biomolecules, Faculty of Medicine of Sobral, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil. ziullec@gmail.com

ABSTRACT
Lectins are a structurally heterogeneous group of proteins that have specific binding sites for carbohydrates and glycoconjugates. Because of their biotechnological potential, lectins are widely used in biomedical research. The present study aimed to evaluate the healing potential of the lectin isolated from the marine red alga Bryothamnion seaforthii (BSL). The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry. For healing tests, skin wounds were induced in the dorsal thoracic region of mice. These animals were randomly divided into three groups and subjected to topical treatment for 12 days with BSL, bovine serum albumin and 150 mM NaCl. To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively. The parameters evaluated included the wound area, the proportion of wound closure and the histological diagnosis. The wound closure was more effective with BSL (Postoperative Day 7 and 12) than controls. The luminal epithelium was completely restructured; the presence of collagen in the dermis and the strongly active presence of young skin annexes demonstrate the potential of treatment with BSL compared with controls. Our findings suggest that BSL has pro-healing properties and can be a potential medical process in the treatment of acute wounds.

Show MeSH
Related in: MedlinePlus