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Characterization of isoforms of the lectin isolated from the red algae Bryothamnion seaforthii and its pro-healing effect.

do Nascimento-Neto LG, Carneiro RF, da Silva SR, da Silva BR, Vassiliepe Sousa Arruda F, Carneiro VA, do Nascimento KS, Saker-Sampaio S, da Silva VA, Porto AL, Cavada BS, Sampaio AH, Teixeira EH, Nagano CS - Mar Drugs (2012)

Bottom Line: Because of their biotechnological potential, lectins are widely used in biomedical research.The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry.To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively.

View Article: PubMed Central - PubMed

Affiliation: LIBS, Integrated Laboratory of Biomolecules, Faculty of Medicine of Sobral, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil. ziullec@gmail.com

ABSTRACT
Lectins are a structurally heterogeneous group of proteins that have specific binding sites for carbohydrates and glycoconjugates. Because of their biotechnological potential, lectins are widely used in biomedical research. The present study aimed to evaluate the healing potential of the lectin isolated from the marine red alga Bryothamnion seaforthii (BSL). The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry. For healing tests, skin wounds were induced in the dorsal thoracic region of mice. These animals were randomly divided into three groups and subjected to topical treatment for 12 days with BSL, bovine serum albumin and 150 mM NaCl. To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively. The parameters evaluated included the wound area, the proportion of wound closure and the histological diagnosis. The wound closure was more effective with BSL (Postoperative Day 7 and 12) than controls. The luminal epithelium was completely restructured; the presence of collagen in the dermis and the strongly active presence of young skin annexes demonstrate the potential of treatment with BSL compared with controls. Our findings suggest that BSL has pro-healing properties and can be a potential medical process in the treatment of acute wounds.

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Photomicrographs of surgically induced skin wounds in mice treated with a topical administration of BSA and BSL in POD 2 (Stain: hematoxylin-eosin and Masson’s trichrome). (A) (BSA). Note the presence of a scab (white star) covering the wound and intense inflammatory exudate. Below the exudate, a collagenolysis area and edema of the reticular dermis are observed (black star). Immediately below the dermis is the subcutaneous muscle layer with congested vessels (arrows). A 4× objective was used; (B) (BSA). Note the presence of a thicker crust (white star) covering the wound bed and a less-intense inflammatory exudate. Below the exudate, a collagenolysis area, edema of the reticular dermis and intense inflammatory infiltrate are observed (black star) with degeneration fatty tissue (DAT). Note the muscle fibers spaced by muscle tissue and fat cells with congested vessels near the collagenolysis area (red arrow). A 4× objective was used; (C) (BSL). A thick scab covers the wound bed (white star). Note the congested vessels of the granulation tissue (arrow) in the reactional adipose tissue (RAT). A 10× objective was used; (D) (BSL). Detail from the previous photo showing vessels of the granulation tissue in areas of intense inflammatory exudate (star). Observe the possible diapedesis of the neutrophil PMNs into the inflamed area (red arrow). A 40× objective was used.
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marinedrugs-10-01936-f007: Photomicrographs of surgically induced skin wounds in mice treated with a topical administration of BSA and BSL in POD 2 (Stain: hematoxylin-eosin and Masson’s trichrome). (A) (BSA). Note the presence of a scab (white star) covering the wound and intense inflammatory exudate. Below the exudate, a collagenolysis area and edema of the reticular dermis are observed (black star). Immediately below the dermis is the subcutaneous muscle layer with congested vessels (arrows). A 4× objective was used; (B) (BSA). Note the presence of a thicker crust (white star) covering the wound bed and a less-intense inflammatory exudate. Below the exudate, a collagenolysis area, edema of the reticular dermis and intense inflammatory infiltrate are observed (black star) with degeneration fatty tissue (DAT). Note the muscle fibers spaced by muscle tissue and fat cells with congested vessels near the collagenolysis area (red arrow). A 4× objective was used; (C) (BSL). A thick scab covers the wound bed (white star). Note the congested vessels of the granulation tissue (arrow) in the reactional adipose tissue (RAT). A 10× objective was used; (D) (BSL). Detail from the previous photo showing vessels of the granulation tissue in areas of intense inflammatory exudate (star). Observe the possible diapedesis of the neutrophil PMNs into the inflamed area (red arrow). A 40× objective was used.

Mentions: The study revealed that the wounds treated with BSA and BSL exhibited scabs sealing the epithelium opening on POD 2 (Figure 7). During the histopathological diagnosis, wounds treated with BSL showed intense inflammatory exudates in the reactional adipose tissue, with a mild inflammatory infiltrate in the reticular dermis in addition to the presence of granulation tissue in the reactional adipose tissue (Figure 7C,D). In wounds treated with BSA, there were intense inflammatory exudates, collagenolysis, and edema of the reticular dermis with the degenerative adipose tissue showing the beginning of a poor healing (Figure 7A,B).


Characterization of isoforms of the lectin isolated from the red algae Bryothamnion seaforthii and its pro-healing effect.

do Nascimento-Neto LG, Carneiro RF, da Silva SR, da Silva BR, Vassiliepe Sousa Arruda F, Carneiro VA, do Nascimento KS, Saker-Sampaio S, da Silva VA, Porto AL, Cavada BS, Sampaio AH, Teixeira EH, Nagano CS - Mar Drugs (2012)

Photomicrographs of surgically induced skin wounds in mice treated with a topical administration of BSA and BSL in POD 2 (Stain: hematoxylin-eosin and Masson’s trichrome). (A) (BSA). Note the presence of a scab (white star) covering the wound and intense inflammatory exudate. Below the exudate, a collagenolysis area and edema of the reticular dermis are observed (black star). Immediately below the dermis is the subcutaneous muscle layer with congested vessels (arrows). A 4× objective was used; (B) (BSA). Note the presence of a thicker crust (white star) covering the wound bed and a less-intense inflammatory exudate. Below the exudate, a collagenolysis area, edema of the reticular dermis and intense inflammatory infiltrate are observed (black star) with degeneration fatty tissue (DAT). Note the muscle fibers spaced by muscle tissue and fat cells with congested vessels near the collagenolysis area (red arrow). A 4× objective was used; (C) (BSL). A thick scab covers the wound bed (white star). Note the congested vessels of the granulation tissue (arrow) in the reactional adipose tissue (RAT). A 10× objective was used; (D) (BSL). Detail from the previous photo showing vessels of the granulation tissue in areas of intense inflammatory exudate (star). Observe the possible diapedesis of the neutrophil PMNs into the inflamed area (red arrow). A 40× objective was used.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3475265&req=5

marinedrugs-10-01936-f007: Photomicrographs of surgically induced skin wounds in mice treated with a topical administration of BSA and BSL in POD 2 (Stain: hematoxylin-eosin and Masson’s trichrome). (A) (BSA). Note the presence of a scab (white star) covering the wound and intense inflammatory exudate. Below the exudate, a collagenolysis area and edema of the reticular dermis are observed (black star). Immediately below the dermis is the subcutaneous muscle layer with congested vessels (arrows). A 4× objective was used; (B) (BSA). Note the presence of a thicker crust (white star) covering the wound bed and a less-intense inflammatory exudate. Below the exudate, a collagenolysis area, edema of the reticular dermis and intense inflammatory infiltrate are observed (black star) with degeneration fatty tissue (DAT). Note the muscle fibers spaced by muscle tissue and fat cells with congested vessels near the collagenolysis area (red arrow). A 4× objective was used; (C) (BSL). A thick scab covers the wound bed (white star). Note the congested vessels of the granulation tissue (arrow) in the reactional adipose tissue (RAT). A 10× objective was used; (D) (BSL). Detail from the previous photo showing vessels of the granulation tissue in areas of intense inflammatory exudate (star). Observe the possible diapedesis of the neutrophil PMNs into the inflamed area (red arrow). A 40× objective was used.
Mentions: The study revealed that the wounds treated with BSA and BSL exhibited scabs sealing the epithelium opening on POD 2 (Figure 7). During the histopathological diagnosis, wounds treated with BSL showed intense inflammatory exudates in the reactional adipose tissue, with a mild inflammatory infiltrate in the reticular dermis in addition to the presence of granulation tissue in the reactional adipose tissue (Figure 7C,D). In wounds treated with BSA, there were intense inflammatory exudates, collagenolysis, and edema of the reticular dermis with the degenerative adipose tissue showing the beginning of a poor healing (Figure 7A,B).

Bottom Line: Because of their biotechnological potential, lectins are widely used in biomedical research.The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry.To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively.

View Article: PubMed Central - PubMed

Affiliation: LIBS, Integrated Laboratory of Biomolecules, Faculty of Medicine of Sobral, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil. ziullec@gmail.com

ABSTRACT
Lectins are a structurally heterogeneous group of proteins that have specific binding sites for carbohydrates and glycoconjugates. Because of their biotechnological potential, lectins are widely used in biomedical research. The present study aimed to evaluate the healing potential of the lectin isolated from the marine red alga Bryothamnion seaforthii (BSL). The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry. For healing tests, skin wounds were induced in the dorsal thoracic region of mice. These animals were randomly divided into three groups and subjected to topical treatment for 12 days with BSL, bovine serum albumin and 150 mM NaCl. To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively. The parameters evaluated included the wound area, the proportion of wound closure and the histological diagnosis. The wound closure was more effective with BSL (Postoperative Day 7 and 12) than controls. The luminal epithelium was completely restructured; the presence of collagen in the dermis and the strongly active presence of young skin annexes demonstrate the potential of treatment with BSL compared with controls. Our findings suggest that BSL has pro-healing properties and can be a potential medical process in the treatment of acute wounds.

Show MeSH
Related in: MedlinePlus