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Characterization of isoforms of the lectin isolated from the red algae Bryothamnion seaforthii and its pro-healing effect.

do Nascimento-Neto LG, Carneiro RF, da Silva SR, da Silva BR, Vassiliepe Sousa Arruda F, Carneiro VA, do Nascimento KS, Saker-Sampaio S, da Silva VA, Porto AL, Cavada BS, Sampaio AH, Teixeira EH, Nagano CS - Mar Drugs (2012)

Bottom Line: Because of their biotechnological potential, lectins are widely used in biomedical research.The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry.To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively.

View Article: PubMed Central - PubMed

Affiliation: LIBS, Integrated Laboratory of Biomolecules, Faculty of Medicine of Sobral, Federal University of CearĂ¡, Fortaleza, CE 60020-181, Brazil. ziullec@gmail.com

ABSTRACT
Lectins are a structurally heterogeneous group of proteins that have specific binding sites for carbohydrates and glycoconjugates. Because of their biotechnological potential, lectins are widely used in biomedical research. The present study aimed to evaluate the healing potential of the lectin isolated from the marine red alga Bryothamnion seaforthii (BSL). The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry. For healing tests, skin wounds were induced in the dorsal thoracic region of mice. These animals were randomly divided into three groups and subjected to topical treatment for 12 days with BSL, bovine serum albumin and 150 mM NaCl. To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively. The parameters evaluated included the wound area, the proportion of wound closure and the histological diagnosis. The wound closure was more effective with BSL (Postoperative Day 7 and 12) than controls. The luminal epithelium was completely restructured; the presence of collagen in the dermis and the strongly active presence of young skin annexes demonstrate the potential of treatment with BSL compared with controls. Our findings suggest that BSL has pro-healing properties and can be a potential medical process in the treatment of acute wounds.

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Related in: MedlinePlus

Complete amino acid sequence of BSL 2, BSL 3 and BSL 4. The tryptic and chymotryptic peptides are represented by T and Q, respectively. The underlined residues represent micro heterogeneity of the isoform sequences.
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marinedrugs-10-01936-f004: Complete amino acid sequence of BSL 2, BSL 3 and BSL 4. The tryptic and chymotryptic peptides are represented by T and Q, respectively. The underlined residues represent micro heterogeneity of the isoform sequences.

Mentions: The complete amino acid sequences of BSL2, BSL3 and BSL4 were determined using tandem mass spectrometry (MS/MS) by overlapping the sequences of the peptides generated by digestion of CA-BSL with trypsin and chymotrypsin (Figure 4, Table 1). Sequence heterogeneity was observed at positions 8 (S/P), 23 (T/P), 27 (V/P), 31 (D/S), 47 (S/H), 49 (P/L) and 57 (A/V).


Characterization of isoforms of the lectin isolated from the red algae Bryothamnion seaforthii and its pro-healing effect.

do Nascimento-Neto LG, Carneiro RF, da Silva SR, da Silva BR, Vassiliepe Sousa Arruda F, Carneiro VA, do Nascimento KS, Saker-Sampaio S, da Silva VA, Porto AL, Cavada BS, Sampaio AH, Teixeira EH, Nagano CS - Mar Drugs (2012)

Complete amino acid sequence of BSL 2, BSL 3 and BSL 4. The tryptic and chymotryptic peptides are represented by T and Q, respectively. The underlined residues represent micro heterogeneity of the isoform sequences.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475265&req=5

marinedrugs-10-01936-f004: Complete amino acid sequence of BSL 2, BSL 3 and BSL 4. The tryptic and chymotryptic peptides are represented by T and Q, respectively. The underlined residues represent micro heterogeneity of the isoform sequences.
Mentions: The complete amino acid sequences of BSL2, BSL3 and BSL4 were determined using tandem mass spectrometry (MS/MS) by overlapping the sequences of the peptides generated by digestion of CA-BSL with trypsin and chymotrypsin (Figure 4, Table 1). Sequence heterogeneity was observed at positions 8 (S/P), 23 (T/P), 27 (V/P), 31 (D/S), 47 (S/H), 49 (P/L) and 57 (A/V).

Bottom Line: Because of their biotechnological potential, lectins are widely used in biomedical research.The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry.To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively.

View Article: PubMed Central - PubMed

Affiliation: LIBS, Integrated Laboratory of Biomolecules, Faculty of Medicine of Sobral, Federal University of CearĂ¡, Fortaleza, CE 60020-181, Brazil. ziullec@gmail.com

ABSTRACT
Lectins are a structurally heterogeneous group of proteins that have specific binding sites for carbohydrates and glycoconjugates. Because of their biotechnological potential, lectins are widely used in biomedical research. The present study aimed to evaluate the healing potential of the lectin isolated from the marine red alga Bryothamnion seaforthii (BSL). The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry. For healing tests, skin wounds were induced in the dorsal thoracic region of mice. These animals were randomly divided into three groups and subjected to topical treatment for 12 days with BSL, bovine serum albumin and 150 mM NaCl. To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively. The parameters evaluated included the wound area, the proportion of wound closure and the histological diagnosis. The wound closure was more effective with BSL (Postoperative Day 7 and 12) than controls. The luminal epithelium was completely restructured; the presence of collagen in the dermis and the strongly active presence of young skin annexes demonstrate the potential of treatment with BSL compared with controls. Our findings suggest that BSL has pro-healing properties and can be a potential medical process in the treatment of acute wounds.

Show MeSH
Related in: MedlinePlus