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Hypocholesterolemic effects of lactic acid-fermented soymilk on rats fed a high cholesterol diet.

Kobayashi M, Hirahata R, Egusa S, Fukuda M - Nutrients (2012)

Bottom Line: The hepatic triglyceride and cholesterol levels in the F-5 and F-10 groups were significantly lowered compared to those in the control group.The plasma total cholesterol level of the F-10 group was significantly decreased.The expression of SREBP-2, a cholesterol synthesis-related gene, was significantly decreased in liver of the F-5 group, but the expression of CYP7a1, a cholesterol catabolism-related gene, was significantly increased.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women's University, Ikebiraki-cho, Nishinomiya, Hyogo, Japan. maki1002@mukogawa-u.ac.jp

ABSTRACT
The effect of fermented soymilk on rats fed a high cholesterol diet was investigated to clarify the cholesterol-lowering function. Male Sprague-Dawley rats aged 7 weeks were fed a control diet (1% cholesterol, high cholesterol diet), high cholesterol diet containing 11.7% fermented soymilk diet (5% soy protein as final concentration, F-5), or high cholesterol diet containing 23.4% fermented soymilk diet (10% soy protein as final concentration, F-10) for 5 weeks. The liver weight and fat mass were decreased by the ingestion of fermented soymilk. The hepatic triglyceride and cholesterol levels in the F-5 and F-10 groups were significantly lowered compared to those in the control group. The plasma total cholesterol level of the F-10 group was significantly decreased. The expression of SREBP-2, a cholesterol synthesis-related gene, was significantly decreased in liver of the F-5 group, but the expression of CYP7a1, a cholesterol catabolism-related gene, was significantly increased. These results suggest that fermented soymilk can modulate the cholesterol metabolism in rats fed a high cholesterol diet.

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Expression of lipid metabolism-related genes in the liver of rats fed experimental diets for 5 weeks. The expression of mRNA was quantitatively measured by real-time RT-PCR. Each value is the mean ± SE for 8 rats. The data were normalized to GAPDH RNA expression and are presented as a ratio to the C value. Statistically significant compared with the control group (* p < 0.05; Student’s t-test).
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nutrients-04-01304-f004: Expression of lipid metabolism-related genes in the liver of rats fed experimental diets for 5 weeks. The expression of mRNA was quantitatively measured by real-time RT-PCR. Each value is the mean ± SE for 8 rats. The data were normalized to GAPDH RNA expression and are presented as a ratio to the C value. Statistically significant compared with the control group (* p < 0.05; Student’s t-test).

Mentions: With regard to the change of hepatic lipid profile by the ingestion of fermented soymilk, the gene expression of hepatic lipid metabolism in the only F-5 group was compared with those of the C group because the gene expression from livers of the F-10 group was not obtained (Figure 4). The gene expression of LXRα was scarcely changed in the F-5 group (1.1 fold). In cholesterol metabolism, the expression of CYP7a1 of the F-5 group was significantly up-regulated to 2.0 fold compared with that of the C group. In contrast, the expression of SREBP-2 was significantly decreased to 0.7 fold in the F-5 group compared with the C group. Although the gene expressions of SREBP-1 and FAS in fatty acid synthesis-related metabolism were down-regulated 0.8 and 0.7 fold, respectively, significant change of those expressions was not found in the F-5 group.


Hypocholesterolemic effects of lactic acid-fermented soymilk on rats fed a high cholesterol diet.

Kobayashi M, Hirahata R, Egusa S, Fukuda M - Nutrients (2012)

Expression of lipid metabolism-related genes in the liver of rats fed experimental diets for 5 weeks. The expression of mRNA was quantitatively measured by real-time RT-PCR. Each value is the mean ± SE for 8 rats. The data were normalized to GAPDH RNA expression and are presented as a ratio to the C value. Statistically significant compared with the control group (* p < 0.05; Student’s t-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475240&req=5

nutrients-04-01304-f004: Expression of lipid metabolism-related genes in the liver of rats fed experimental diets for 5 weeks. The expression of mRNA was quantitatively measured by real-time RT-PCR. Each value is the mean ± SE for 8 rats. The data were normalized to GAPDH RNA expression and are presented as a ratio to the C value. Statistically significant compared with the control group (* p < 0.05; Student’s t-test).
Mentions: With regard to the change of hepatic lipid profile by the ingestion of fermented soymilk, the gene expression of hepatic lipid metabolism in the only F-5 group was compared with those of the C group because the gene expression from livers of the F-10 group was not obtained (Figure 4). The gene expression of LXRα was scarcely changed in the F-5 group (1.1 fold). In cholesterol metabolism, the expression of CYP7a1 of the F-5 group was significantly up-regulated to 2.0 fold compared with that of the C group. In contrast, the expression of SREBP-2 was significantly decreased to 0.7 fold in the F-5 group compared with the C group. Although the gene expressions of SREBP-1 and FAS in fatty acid synthesis-related metabolism were down-regulated 0.8 and 0.7 fold, respectively, significant change of those expressions was not found in the F-5 group.

Bottom Line: The hepatic triglyceride and cholesterol levels in the F-5 and F-10 groups were significantly lowered compared to those in the control group.The plasma total cholesterol level of the F-10 group was significantly decreased.The expression of SREBP-2, a cholesterol synthesis-related gene, was significantly decreased in liver of the F-5 group, but the expression of CYP7a1, a cholesterol catabolism-related gene, was significantly increased.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women's University, Ikebiraki-cho, Nishinomiya, Hyogo, Japan. maki1002@mukogawa-u.ac.jp

ABSTRACT
The effect of fermented soymilk on rats fed a high cholesterol diet was investigated to clarify the cholesterol-lowering function. Male Sprague-Dawley rats aged 7 weeks were fed a control diet (1% cholesterol, high cholesterol diet), high cholesterol diet containing 11.7% fermented soymilk diet (5% soy protein as final concentration, F-5), or high cholesterol diet containing 23.4% fermented soymilk diet (10% soy protein as final concentration, F-10) for 5 weeks. The liver weight and fat mass were decreased by the ingestion of fermented soymilk. The hepatic triglyceride and cholesterol levels in the F-5 and F-10 groups were significantly lowered compared to those in the control group. The plasma total cholesterol level of the F-10 group was significantly decreased. The expression of SREBP-2, a cholesterol synthesis-related gene, was significantly decreased in liver of the F-5 group, but the expression of CYP7a1, a cholesterol catabolism-related gene, was significantly increased. These results suggest that fermented soymilk can modulate the cholesterol metabolism in rats fed a high cholesterol diet.

Show MeSH
Related in: MedlinePlus