Limits...
Effects of glycerol and creatine hyperhydration on doping-relevant blood parameters.

Polyviou TP, Easton C, Beis L, Malkova D, Takas P, Hambly C, Speakman JR, Koehler K, Pitsiladis YP - Nutrients (2012)

Bottom Line: However, the scientific basis of the inclusion of Gly as a "masking agent" remains inconclusive.This hyperhydration did not significantly alter plasma volume or any of the doping-relevant blood parameters (e.g., hematocrit, Hb, reticulocytes and total Hb-mass) even when Gly was clearly detectable in urine samples.In conclusion, this study shows that supplementation with hyperhydrating solution containing Gly for 7 days does not significantly alter doping-relevant blood parameters.

View Article: PubMed Central - PubMed

Affiliation: Institute of Cardiovascular & Medical Sciences, College of Medicine, Veterinary and Life Sciences, University of Glasgow, UK. t.polyviou.1@research.gla.ac.uk

ABSTRACT
Glycerol is prohibited as an ergogenic aid by the World Anti-Doping Agency (WADA) due to the potential for its plasma expansion properties to have masking effects. However, the scientific basis of the inclusion of Gly as a "masking agent" remains inconclusive. The purpose of this study was to determine the effects of a hyperhydrating supplement containing Gly on doping-relevant blood parameters. Nine trained males ingested a hyperhydrating mixture twice per day for 7 days containing 1.0 g·kg(-1) body mass (BM) of Gly, 10.0 g of creatine and 75.0 g of glucose. Blood samples were collected and total hemoglobin (Hb) mass determined using the optimized carbon monoxide (CO) rebreathing method pre- and post-supplementation. BM and total body water (TBW) increased significantly following supplementation by 1.1 ± 1.2 and 1.0 ± 1.2 L (BM, P < 0.01; TBW, P <0.01), respectively. This hyperhydration did not significantly alter plasma volume or any of the doping-relevant blood parameters (e.g., hematocrit, Hb, reticulocytes and total Hb-mass) even when Gly was clearly detectable in urine samples. In conclusion, this study shows that supplementation with hyperhydrating solution containing Gly for 7 days does not significantly alter doping-relevant blood parameters.

Show MeSH

Related in: MedlinePlus

Plasma volume (PV) (mL), total Hb mass (tHb-mass), total hemoglobin (Hb) (g·dL−1) and hematocrit (Hct) (%) pre- and post-supplementation with the hyperhydrating solution containing Gly, Cr and Glu. Data presented as Mean ± S.D., N = 9.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3475229&req=5

nutrients-04-01171-f004: Plasma volume (PV) (mL), total Hb mass (tHb-mass), total hemoglobin (Hb) (g·dL−1) and hematocrit (Hct) (%) pre- and post-supplementation with the hyperhydrating solution containing Gly, Cr and Glu. Data presented as Mean ± S.D., N = 9.

Mentions: In order to provide an overview of the effects of 7-day supplementation with the hyperhydrating solution containing Gly, Cr and Glu on blood parameters, pre- and post-supplementation values of PV, tHb-mass, Hb and Hct are presented in Figure 4. PV (Pre: 4246.6 ± 424.0 mL, Post: 4274.1 ± 457.7 mL; P = 0.7), tHb-mass (Pre: 936.1 ± 98.6 g, Post: 933.2 ± 94.2 g; P = 0.8), Hb (Pre: 14.3 ± 0.7 g·dL−1, Post: 14.3 ± 0.9 g·dL−1; P =0.9) or Hct (Pre: 41.9 ± 1.5%, Post: 41.7 ± 2.3%; P = 0.8) were not significantly different between pre- and post-supplementation.


Effects of glycerol and creatine hyperhydration on doping-relevant blood parameters.

Polyviou TP, Easton C, Beis L, Malkova D, Takas P, Hambly C, Speakman JR, Koehler K, Pitsiladis YP - Nutrients (2012)

Plasma volume (PV) (mL), total Hb mass (tHb-mass), total hemoglobin (Hb) (g·dL−1) and hematocrit (Hct) (%) pre- and post-supplementation with the hyperhydrating solution containing Gly, Cr and Glu. Data presented as Mean ± S.D., N = 9.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475229&req=5

nutrients-04-01171-f004: Plasma volume (PV) (mL), total Hb mass (tHb-mass), total hemoglobin (Hb) (g·dL−1) and hematocrit (Hct) (%) pre- and post-supplementation with the hyperhydrating solution containing Gly, Cr and Glu. Data presented as Mean ± S.D., N = 9.
Mentions: In order to provide an overview of the effects of 7-day supplementation with the hyperhydrating solution containing Gly, Cr and Glu on blood parameters, pre- and post-supplementation values of PV, tHb-mass, Hb and Hct are presented in Figure 4. PV (Pre: 4246.6 ± 424.0 mL, Post: 4274.1 ± 457.7 mL; P = 0.7), tHb-mass (Pre: 936.1 ± 98.6 g, Post: 933.2 ± 94.2 g; P = 0.8), Hb (Pre: 14.3 ± 0.7 g·dL−1, Post: 14.3 ± 0.9 g·dL−1; P =0.9) or Hct (Pre: 41.9 ± 1.5%, Post: 41.7 ± 2.3%; P = 0.8) were not significantly different between pre- and post-supplementation.

Bottom Line: However, the scientific basis of the inclusion of Gly as a "masking agent" remains inconclusive.This hyperhydration did not significantly alter plasma volume or any of the doping-relevant blood parameters (e.g., hematocrit, Hb, reticulocytes and total Hb-mass) even when Gly was clearly detectable in urine samples.In conclusion, this study shows that supplementation with hyperhydrating solution containing Gly for 7 days does not significantly alter doping-relevant blood parameters.

View Article: PubMed Central - PubMed

Affiliation: Institute of Cardiovascular & Medical Sciences, College of Medicine, Veterinary and Life Sciences, University of Glasgow, UK. t.polyviou.1@research.gla.ac.uk

ABSTRACT
Glycerol is prohibited as an ergogenic aid by the World Anti-Doping Agency (WADA) due to the potential for its plasma expansion properties to have masking effects. However, the scientific basis of the inclusion of Gly as a "masking agent" remains inconclusive. The purpose of this study was to determine the effects of a hyperhydrating supplement containing Gly on doping-relevant blood parameters. Nine trained males ingested a hyperhydrating mixture twice per day for 7 days containing 1.0 g·kg(-1) body mass (BM) of Gly, 10.0 g of creatine and 75.0 g of glucose. Blood samples were collected and total hemoglobin (Hb) mass determined using the optimized carbon monoxide (CO) rebreathing method pre- and post-supplementation. BM and total body water (TBW) increased significantly following supplementation by 1.1 ± 1.2 and 1.0 ± 1.2 L (BM, P < 0.01; TBW, P <0.01), respectively. This hyperhydration did not significantly alter plasma volume or any of the doping-relevant blood parameters (e.g., hematocrit, Hb, reticulocytes and total Hb-mass) even when Gly was clearly detectable in urine samples. In conclusion, this study shows that supplementation with hyperhydrating solution containing Gly for 7 days does not significantly alter doping-relevant blood parameters.

Show MeSH
Related in: MedlinePlus