Limits...
A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways.

Chien YC, Sheu MJ, Wu CH, Lin WH, Chen YY, Cheng PL, Cheng HC - BMC Complement Altern Med (2012)

Bottom Line: Our results showed that GLY significantly decreased the thickness of neointima.The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK.These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Life Sciences, and Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.

ABSTRACT

Background: This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.

Methods: VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.

Results: Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.

Conclusions: These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

Show MeSH

Related in: MedlinePlus

Responses of rat carotid arteries to balloon injury, and the effects of extract of GLY on balloon injury. (A). The left panel represents the low-power (100×) observations from a balloon-injured vessel, a balloon-injured vessel treated with GLY at 0.5 g/ml,and uninjruied vessel (sham control). And the right panel represents the high-power (200×). (L, lumen; N, neointima; M, medium.). (B). The control group shows a significantly higher neointima to media ratio as compared with the groups treated with extract of GLY at 0.5 g/mL. Values are means of two separate experiments, with standard errors represented by vertical bars. Mean value was significantly different from that of the control group:* p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3475136&req=5

Figure 5: Responses of rat carotid arteries to balloon injury, and the effects of extract of GLY on balloon injury. (A). The left panel represents the low-power (100×) observations from a balloon-injured vessel, a balloon-injured vessel treated with GLY at 0.5 g/ml,and uninjruied vessel (sham control). And the right panel represents the high-power (200×). (L, lumen; N, neointima; M, medium.). (B). The control group shows a significantly higher neointima to media ratio as compared with the groups treated with extract of GLY at 0.5 g/mL. Values are means of two separate experiments, with standard errors represented by vertical bars. Mean value was significantly different from that of the control group:* p < 0.05.

Mentions: To test the efficacy of GLY in inhibiting neointima formation, Sprague–Dawley rats were applied with different concentrations of GLY (0.5 g/mL) for 14 days following balloon injury. After 2 weeks of balloon injury, the injured arteries were harvested and subjected to histological analysis for neointima formation assay. Intimal hyperplasia induced by balloon injury was evident as compared with the normal control (Figure5A). The present results showed that GLY (0.5 g/mL) were effective in preventing neointima formation (Figure5B). However, GLY at 0.125 g/mL and 0.25 g/mL did not show any influence on balloon injury-induced neointimal formation (data not shown). Using computerized image analysis, we calculated the area ratio of intimal and media layers; we found a reduction of 24% in the area ratio of GLY-treated groups as compared with the balloon-injured control group by GLY at 0.5 g/mL of GLY, respectively (Figure5B).


A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways.

Chien YC, Sheu MJ, Wu CH, Lin WH, Chen YY, Cheng PL, Cheng HC - BMC Complement Altern Med (2012)

Responses of rat carotid arteries to balloon injury, and the effects of extract of GLY on balloon injury. (A). The left panel represents the low-power (100×) observations from a balloon-injured vessel, a balloon-injured vessel treated with GLY at 0.5 g/ml,and uninjruied vessel (sham control). And the right panel represents the high-power (200×). (L, lumen; N, neointima; M, medium.). (B). The control group shows a significantly higher neointima to media ratio as compared with the groups treated with extract of GLY at 0.5 g/mL. Values are means of two separate experiments, with standard errors represented by vertical bars. Mean value was significantly different from that of the control group:* p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475136&req=5

Figure 5: Responses of rat carotid arteries to balloon injury, and the effects of extract of GLY on balloon injury. (A). The left panel represents the low-power (100×) observations from a balloon-injured vessel, a balloon-injured vessel treated with GLY at 0.5 g/ml,and uninjruied vessel (sham control). And the right panel represents the high-power (200×). (L, lumen; N, neointima; M, medium.). (B). The control group shows a significantly higher neointima to media ratio as compared with the groups treated with extract of GLY at 0.5 g/mL. Values are means of two separate experiments, with standard errors represented by vertical bars. Mean value was significantly different from that of the control group:* p < 0.05.
Mentions: To test the efficacy of GLY in inhibiting neointima formation, Sprague–Dawley rats were applied with different concentrations of GLY (0.5 g/mL) for 14 days following balloon injury. After 2 weeks of balloon injury, the injured arteries were harvested and subjected to histological analysis for neointima formation assay. Intimal hyperplasia induced by balloon injury was evident as compared with the normal control (Figure5A). The present results showed that GLY (0.5 g/mL) were effective in preventing neointima formation (Figure5B). However, GLY at 0.125 g/mL and 0.25 g/mL did not show any influence on balloon injury-induced neointimal formation (data not shown). Using computerized image analysis, we calculated the area ratio of intimal and media layers; we found a reduction of 24% in the area ratio of GLY-treated groups as compared with the balloon-injured control group by GLY at 0.5 g/mL of GLY, respectively (Figure5B).

Bottom Line: Our results showed that GLY significantly decreased the thickness of neointima.The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK.These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Life Sciences, and Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.

ABSTRACT

Background: This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.

Methods: VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.

Results: Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.

Conclusions: These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

Show MeSH
Related in: MedlinePlus