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A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways.

Chien YC, Sheu MJ, Wu CH, Lin WH, Chen YY, Cheng PL, Cheng HC - BMC Complement Altern Med (2012)

Bottom Line: Our results showed that GLY significantly decreased the thickness of neointima.The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK.These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Life Sciences, and Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.

ABSTRACT

Background: This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.

Methods: VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.

Results: Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.

Conclusions: These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

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Related in: MedlinePlus

GLY inhibits the release of MMP-2 and MMP-9 in VSMCs. (A). To examine the possible antimigration mechanisms of GLY, we determined the activity of MMP-2 and MMP-9 in culture media of VSMCs cells by zymographic analysis. (B). GLY inhibited MMP-9 and MMP-2 activities in a concentration-dependent manner. Values are means of three separate experiments, with standard errors represented by vertical bars. Mean value was significantly different from that of the control group :* p < 0.05,** p < 0.01, *** p < 0.001.
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Figure 3: GLY inhibits the release of MMP-2 and MMP-9 in VSMCs. (A). To examine the possible antimigration mechanisms of GLY, we determined the activity of MMP-2 and MMP-9 in culture media of VSMCs cells by zymographic analysis. (B). GLY inhibited MMP-9 and MMP-2 activities in a concentration-dependent manner. Values are means of three separate experiments, with standard errors represented by vertical bars. Mean value was significantly different from that of the control group :* p < 0.05,** p < 0.01, *** p < 0.001.

Mentions: To examine the possible antimetastatic mechanisms of GLY, we determined the activity of MMP-2 and MMP-9 in culture media of VSMCs cells by zymographic analysis. In the absence of treatment, VSMCs cells constitutively secreted both MMP-9 and MMP-2. As shown in Figure3A, GLY inhibited MMP-9 and MMP-2 activities in a concentration-dependent manner after incubation for 18 h (Figures3 B and3C).


A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways.

Chien YC, Sheu MJ, Wu CH, Lin WH, Chen YY, Cheng PL, Cheng HC - BMC Complement Altern Med (2012)

GLY inhibits the release of MMP-2 and MMP-9 in VSMCs. (A). To examine the possible antimigration mechanisms of GLY, we determined the activity of MMP-2 and MMP-9 in culture media of VSMCs cells by zymographic analysis. (B). GLY inhibited MMP-9 and MMP-2 activities in a concentration-dependent manner. Values are means of three separate experiments, with standard errors represented by vertical bars. Mean value was significantly different from that of the control group :* p < 0.05,** p < 0.01, *** p < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475136&req=5

Figure 3: GLY inhibits the release of MMP-2 and MMP-9 in VSMCs. (A). To examine the possible antimigration mechanisms of GLY, we determined the activity of MMP-2 and MMP-9 in culture media of VSMCs cells by zymographic analysis. (B). GLY inhibited MMP-9 and MMP-2 activities in a concentration-dependent manner. Values are means of three separate experiments, with standard errors represented by vertical bars. Mean value was significantly different from that of the control group :* p < 0.05,** p < 0.01, *** p < 0.001.
Mentions: To examine the possible antimetastatic mechanisms of GLY, we determined the activity of MMP-2 and MMP-9 in culture media of VSMCs cells by zymographic analysis. In the absence of treatment, VSMCs cells constitutively secreted both MMP-9 and MMP-2. As shown in Figure3A, GLY inhibited MMP-9 and MMP-2 activities in a concentration-dependent manner after incubation for 18 h (Figures3 B and3C).

Bottom Line: Our results showed that GLY significantly decreased the thickness of neointima.The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK.These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Life Sciences, and Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.

ABSTRACT

Background: This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.

Methods: VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.

Results: Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.

Conclusions: These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

Show MeSH
Related in: MedlinePlus