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A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways.

Chien YC, Sheu MJ, Wu CH, Lin WH, Chen YY, Cheng PL, Cheng HC - BMC Complement Altern Med (2012)

Bottom Line: Our results showed that GLY significantly decreased the thickness of neointima.The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK.These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Life Sciences, and Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.

ABSTRACT

Background: This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.

Methods: VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.

Results: Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.

Conclusions: These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

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Related in: MedlinePlus

Effects of GLY on wound healing migration of VSMCs cells. Wound was introduced by scraping confluent cell layers with a pipet tip. VSMCs cells were incubated with GLY (0.0625, 0.125, 0.25 and 0.5 mg/mL) for 18 h, and the migration distances of cells were calculated. (A) Representative photographs of invading cells that received either control (15% FBS) or GLY treatment. (B) Migrated cells across the black lines were counted in six random fields from each treatment. The mean number of cells in the denuded zone is quantified by three independent experiments. (C). Effects of GLY on transwell migration assay vascular smooth muscle cells. VSMCs were incubated with GLY (0.0625, 0.125, 0.25 and 0.5 mg/mL) for 18 h, and (D). The transwell migration cells were calculated. Photos of the migration VSMCs cells were taken under a microscope (100-fold magnification). Mean value was significantly different from that of the control group (15% FBS): * p < 0.05,** p < 0.01.
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Figure 2: Effects of GLY on wound healing migration of VSMCs cells. Wound was introduced by scraping confluent cell layers with a pipet tip. VSMCs cells were incubated with GLY (0.0625, 0.125, 0.25 and 0.5 mg/mL) for 18 h, and the migration distances of cells were calculated. (A) Representative photographs of invading cells that received either control (15% FBS) or GLY treatment. (B) Migrated cells across the black lines were counted in six random fields from each treatment. The mean number of cells in the denuded zone is quantified by three independent experiments. (C). Effects of GLY on transwell migration assay vascular smooth muscle cells. VSMCs were incubated with GLY (0.0625, 0.125, 0.25 and 0.5 mg/mL) for 18 h, and (D). The transwell migration cells were calculated. Photos of the migration VSMCs cells were taken under a microscope (100-fold magnification). Mean value was significantly different from that of the control group (15% FBS): * p < 0.05,** p < 0.01.

Mentions: We assessed the effect of GLY on the migration of VSMCS cells using the wound healing assay in which the confluent monolayer was scraped with a sterile micropipet tip to create a scratch wound. As shown in our data, GLY at 0.25 and 0.5 mg/mL inhibited the migration of VSMCS cells (Figures2 A and2B).


A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways.

Chien YC, Sheu MJ, Wu CH, Lin WH, Chen YY, Cheng PL, Cheng HC - BMC Complement Altern Med (2012)

Effects of GLY on wound healing migration of VSMCs cells. Wound was introduced by scraping confluent cell layers with a pipet tip. VSMCs cells were incubated with GLY (0.0625, 0.125, 0.25 and 0.5 mg/mL) for 18 h, and the migration distances of cells were calculated. (A) Representative photographs of invading cells that received either control (15% FBS) or GLY treatment. (B) Migrated cells across the black lines were counted in six random fields from each treatment. The mean number of cells in the denuded zone is quantified by three independent experiments. (C). Effects of GLY on transwell migration assay vascular smooth muscle cells. VSMCs were incubated with GLY (0.0625, 0.125, 0.25 and 0.5 mg/mL) for 18 h, and (D). The transwell migration cells were calculated. Photos of the migration VSMCs cells were taken under a microscope (100-fold magnification). Mean value was significantly different from that of the control group (15% FBS): * p < 0.05,** p < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475136&req=5

Figure 2: Effects of GLY on wound healing migration of VSMCs cells. Wound was introduced by scraping confluent cell layers with a pipet tip. VSMCs cells were incubated with GLY (0.0625, 0.125, 0.25 and 0.5 mg/mL) for 18 h, and the migration distances of cells were calculated. (A) Representative photographs of invading cells that received either control (15% FBS) or GLY treatment. (B) Migrated cells across the black lines were counted in six random fields from each treatment. The mean number of cells in the denuded zone is quantified by three independent experiments. (C). Effects of GLY on transwell migration assay vascular smooth muscle cells. VSMCs were incubated with GLY (0.0625, 0.125, 0.25 and 0.5 mg/mL) for 18 h, and (D). The transwell migration cells were calculated. Photos of the migration VSMCs cells were taken under a microscope (100-fold magnification). Mean value was significantly different from that of the control group (15% FBS): * p < 0.05,** p < 0.01.
Mentions: We assessed the effect of GLY on the migration of VSMCS cells using the wound healing assay in which the confluent monolayer was scraped with a sterile micropipet tip to create a scratch wound. As shown in our data, GLY at 0.25 and 0.5 mg/mL inhibited the migration of VSMCS cells (Figures2 A and2B).

Bottom Line: Our results showed that GLY significantly decreased the thickness of neointima.The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK.These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Life Sciences, and Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.

ABSTRACT

Background: This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.

Methods: VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.

Results: Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.

Conclusions: These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

Show MeSH
Related in: MedlinePlus