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Rubber (Hevea brasiliensis) seed oil toxicity effect and Linamarin compound analysis.

Salimon J, Abdullah BM, Salih N - Lipids Health Dis (2012)

Bottom Line: The results showed that no functional groups such as cyanide (C ≡ N) associated with linamarin were observed.RSO did not show any toxic potential to the rats.This can be attributed no hazardous linamarin were found in RSO.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia. jumat@ukm.my

ABSTRACT

Background: The lipid fraction of rubber (Hevea brasiliensis (kunth. Muell)) seed was extracted and analyzed for toxicological effect. The toxicological compound such as linamarin in rubber seed oil (RSO) extracted using different solvents, such as hexane (RSOh), mixture of chloroform + methanol (RSOchl+mth) and ethanol (RSOeth) were also studied. Various methods analysis such as Fourier transforms infrared spectroscopy (FTIR) and colorimetric methods were carried out to determine the present of such compounds.

Results: FTIR spectrum of RSO did not show any presence of cyanide peak. The determination of cyanide by using colorimetric method was demonstrated no response of the cyanide in RSO and didn't show any colored comparing with commercial cyanide which observed blue color. The results showed that no functional groups such as cyanide (C ≡ N) associated with linamarin were observed. Toxicological test using rats was also conducted to further confirm the absence of such compounds. RSO did not show any toxic potential to the rats. Bioassay experiments using shrimps had been used as test organisms to evaluate the toxicity of linamarin extract from RSO(h,) RSO(chl+mth) and RSO(eth) and LC50 were found to be (211.70 %, 139.40 %, and 117.41 %, respectively).

Conclusions: This can be attributed no hazardous linamarin were found in RSO.

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Related in: MedlinePlus

Colorimetric method reaction.
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Figure 4: Colorimetric method reaction.

Mentions: The detection of small amounts of cyanide, colorimetric method based on the könig reaction was proposed by [16]. This method involves the oxidation of cyanide to cyanogens chloride with chloramines T. Cyanogens chloride was then reacted with pyridine to form N-cyanopyridinium chloride (könig reaction). The N-cyanopyridinium chloride was then reacted with barbituric acid to produce blue color was measured between 570–630 nm [16,24]. This method was preferable for cyanide at 3.8 × 10-5 M. The reaction of the pyridine- barbituric acid method is shown in Figure 4. The same conditions were used to determine the (C ≡ N) in sodium cyanide (NaCN) which was used as a standard to compare it with HCN which was hydrolyzed from RS and RSO samples.


Rubber (Hevea brasiliensis) seed oil toxicity effect and Linamarin compound analysis.

Salimon J, Abdullah BM, Salih N - Lipids Health Dis (2012)

Colorimetric method reaction.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475106&req=5

Figure 4: Colorimetric method reaction.
Mentions: The detection of small amounts of cyanide, colorimetric method based on the könig reaction was proposed by [16]. This method involves the oxidation of cyanide to cyanogens chloride with chloramines T. Cyanogens chloride was then reacted with pyridine to form N-cyanopyridinium chloride (könig reaction). The N-cyanopyridinium chloride was then reacted with barbituric acid to produce blue color was measured between 570–630 nm [16,24]. This method was preferable for cyanide at 3.8 × 10-5 M. The reaction of the pyridine- barbituric acid method is shown in Figure 4. The same conditions were used to determine the (C ≡ N) in sodium cyanide (NaCN) which was used as a standard to compare it with HCN which was hydrolyzed from RS and RSO samples.

Bottom Line: The results showed that no functional groups such as cyanide (C ≡ N) associated with linamarin were observed.RSO did not show any toxic potential to the rats.This can be attributed no hazardous linamarin were found in RSO.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia. jumat@ukm.my

ABSTRACT

Background: The lipid fraction of rubber (Hevea brasiliensis (kunth. Muell)) seed was extracted and analyzed for toxicological effect. The toxicological compound such as linamarin in rubber seed oil (RSO) extracted using different solvents, such as hexane (RSOh), mixture of chloroform + methanol (RSOchl+mth) and ethanol (RSOeth) were also studied. Various methods analysis such as Fourier transforms infrared spectroscopy (FTIR) and colorimetric methods were carried out to determine the present of such compounds.

Results: FTIR spectrum of RSO did not show any presence of cyanide peak. The determination of cyanide by using colorimetric method was demonstrated no response of the cyanide in RSO and didn't show any colored comparing with commercial cyanide which observed blue color. The results showed that no functional groups such as cyanide (C ≡ N) associated with linamarin were observed. Toxicological test using rats was also conducted to further confirm the absence of such compounds. RSO did not show any toxic potential to the rats. Bioassay experiments using shrimps had been used as test organisms to evaluate the toxicity of linamarin extract from RSO(h,) RSO(chl+mth) and RSO(eth) and LC50 were found to be (211.70 %, 139.40 %, and 117.41 %, respectively).

Conclusions: This can be attributed no hazardous linamarin were found in RSO.

Show MeSH
Related in: MedlinePlus