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Rubber (Hevea brasiliensis) seed oil toxicity effect and Linamarin compound analysis.

Salimon J, Abdullah BM, Salih N - Lipids Health Dis (2012)

Bottom Line: The results showed that no functional groups such as cyanide (C ≡ N) associated with linamarin were observed.RSO did not show any toxic potential to the rats.This can be attributed no hazardous linamarin were found in RSO.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia. jumat@ukm.my

ABSTRACT

Background: The lipid fraction of rubber (Hevea brasiliensis (kunth. Muell)) seed was extracted and analyzed for toxicological effect. The toxicological compound such as linamarin in rubber seed oil (RSO) extracted using different solvents, such as hexane (RSOh), mixture of chloroform + methanol (RSOchl+mth) and ethanol (RSOeth) were also studied. Various methods analysis such as Fourier transforms infrared spectroscopy (FTIR) and colorimetric methods were carried out to determine the present of such compounds.

Results: FTIR spectrum of RSO did not show any presence of cyanide peak. The determination of cyanide by using colorimetric method was demonstrated no response of the cyanide in RSO and didn't show any colored comparing with commercial cyanide which observed blue color. The results showed that no functional groups such as cyanide (C ≡ N) associated with linamarin were observed. Toxicological test using rats was also conducted to further confirm the absence of such compounds. RSO did not show any toxic potential to the rats. Bioassay experiments using shrimps had been used as test organisms to evaluate the toxicity of linamarin extract from RSO(h,) RSO(chl+mth) and RSO(eth) and LC50 were found to be (211.70 %, 139.40 %, and 117.41 %, respectively).

Conclusions: This can be attributed no hazardous linamarin were found in RSO.

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Related in: MedlinePlus

Body weight gain of rats feed RSO and blank control. Note: RSOh: RSO was extracted using hexane as solvent, RSOch+mth: RSO was extracted using mixture of chloroform and methanol as solvent, RSOeth: RSO was extracted using ethanol as solvent, Blank: rats food.
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Figure 3: Body weight gain of rats feed RSO and blank control. Note: RSOh: RSO was extracted using hexane as solvent, RSOch+mth: RSO was extracted using mixture of chloroform and methanol as solvent, RSOeth: RSO was extracted using ethanol as solvent, Blank: rats food.

Mentions: The RSOh, RSOch+mth and RSOeth had no adverse effect on the rats food consumption. A similar increase in the average daily gain of rats fed with RSOh, RSOch+mth and RSOeth was also observed; however, the differences between the 3 white male rats were not statistically significant. The 3 male rats showed no significant difference in body weight gain, food efficiency, body waist measurement and body height measurement. The growth rate of 3 white male rats is shown in Table 5 and Figure 3. These results indicated that RSOh, RSOch+mth and RSOeth had no toxic or antipalatability effects depending on the statistical analysis of p-value (P < 0.05) [2,17].


Rubber (Hevea brasiliensis) seed oil toxicity effect and Linamarin compound analysis.

Salimon J, Abdullah BM, Salih N - Lipids Health Dis (2012)

Body weight gain of rats feed RSO and blank control. Note: RSOh: RSO was extracted using hexane as solvent, RSOch+mth: RSO was extracted using mixture of chloroform and methanol as solvent, RSOeth: RSO was extracted using ethanol as solvent, Blank: rats food.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475106&req=5

Figure 3: Body weight gain of rats feed RSO and blank control. Note: RSOh: RSO was extracted using hexane as solvent, RSOch+mth: RSO was extracted using mixture of chloroform and methanol as solvent, RSOeth: RSO was extracted using ethanol as solvent, Blank: rats food.
Mentions: The RSOh, RSOch+mth and RSOeth had no adverse effect on the rats food consumption. A similar increase in the average daily gain of rats fed with RSOh, RSOch+mth and RSOeth was also observed; however, the differences between the 3 white male rats were not statistically significant. The 3 male rats showed no significant difference in body weight gain, food efficiency, body waist measurement and body height measurement. The growth rate of 3 white male rats is shown in Table 5 and Figure 3. These results indicated that RSOh, RSOch+mth and RSOeth had no toxic or antipalatability effects depending on the statistical analysis of p-value (P < 0.05) [2,17].

Bottom Line: The results showed that no functional groups such as cyanide (C ≡ N) associated with linamarin were observed.RSO did not show any toxic potential to the rats.This can be attributed no hazardous linamarin were found in RSO.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia. jumat@ukm.my

ABSTRACT

Background: The lipid fraction of rubber (Hevea brasiliensis (kunth. Muell)) seed was extracted and analyzed for toxicological effect. The toxicological compound such as linamarin in rubber seed oil (RSO) extracted using different solvents, such as hexane (RSOh), mixture of chloroform + methanol (RSOchl+mth) and ethanol (RSOeth) were also studied. Various methods analysis such as Fourier transforms infrared spectroscopy (FTIR) and colorimetric methods were carried out to determine the present of such compounds.

Results: FTIR spectrum of RSO did not show any presence of cyanide peak. The determination of cyanide by using colorimetric method was demonstrated no response of the cyanide in RSO and didn't show any colored comparing with commercial cyanide which observed blue color. The results showed that no functional groups such as cyanide (C ≡ N) associated with linamarin were observed. Toxicological test using rats was also conducted to further confirm the absence of such compounds. RSO did not show any toxic potential to the rats. Bioassay experiments using shrimps had been used as test organisms to evaluate the toxicity of linamarin extract from RSO(h,) RSO(chl+mth) and RSO(eth) and LC50 were found to be (211.70 %, 139.40 %, and 117.41 %, respectively).

Conclusions: This can be attributed no hazardous linamarin were found in RSO.

Show MeSH
Related in: MedlinePlus